Báo cáo khoa học: Auto-methylation of the mouse DNA-(cytosine C5)-methyltransferase Dnmt3a at its active site cysteine residue pot

... bond between the enzyme and the substrate base. The for- mation of the cysteine cytosine bond increases the neg- ative charge density at the C5 atom of the cytosine, which then attacks the methyl group ... to the methyl group of AdoMet and is the most reactive residue in the catalytic center of the enzyme. To test whether the catalytic cysteine is the...

Ngày tải lên: 14/03/2014, 23:20

... substantially decreased the phosphorylation at Ser64, but the phos- phorylation of the FEMRI variant was similar to that of wild-type 4E-BP1, and that of the FEMNI protein was intermediate between the other two ... shown that the introduction of acidic residues at the positions of the phosphorylation sites in 4E-BP1 decreases the interaction of 4E-BP1 with ra...

Ngày tải lên: 16/03/2014, 06:20

Ngày tải lên: 31/03/2014, 00:20

... the two active- site motifs changed to Ala residues. (A) Schematic representation of the structures of wild-type cathepsin E and its active- site mutant. Asp residues in the two active- site con- sensus ... chains. These results suggest that the nature of the N-glycosylation of cathepsin E varies with cell type or its cellular localization. We thus ana- lyzed t...

Ngày tải lên: 16/03/2014, 14:20

... contain a CCAAT box at 324 bp upstream of the major transcription start site. The presence of a CCAAT box and the absence of a TATA box seem to be one of the characteristics of the mouse integrin ... plasmid. The assays were carried out in triplicate, and the error bars indicate the standard deviation. Fig. 8. Effects of mutations in the Ets- and GATA-binding...

Ngày tải lên: 21/02/2014, 03:20

... the rate constant for the taurine e fflux (min )1 ) at each time point was estimated as the negative slope of the graph between the time point and the proceeding time point. The taurine efflux at ... experimental medium from the time of the initiation of the influx experiment. The influx was estimated f rom the slop e of t he uptake cu rves and i n each case gi...

Ngày tải lên: 30/03/2014, 15:20

... from the NAS substrate. The pattern of expression of these products changed during progression of the hair cycle (Fig. 9). RP-HPLC separation of the reaction products of the HIOMT assay from mouse ... heterogeneity in the cutaneous expression of elements of the serotoninergic pathway. Therefore, we have tested the expression of dif- ferent elements of the...

Ngày tải lên: 31/03/2014, 07:20

... All patients treated in our hospital suffered at least loss of one rel- ative, for example, a partner or child, and two mothers of our cohort lost both of their children. The majority of patients com- plained ... Department of Psychotraumatology of the University of Heidelberg (Germany) assembled a comprehen- sive list of 400 qualified psychotherapists offering immediate...

Ngày tải lên: 25/10/2012, 10:41

... up–down conformational change of SERCA) was rarely observed and that the lifetime of the elevated conformation was apparently increased. The increased lifetime of the ele- vated conformation at low Ca 2+ concentration ... conformation), and the number of peaks must correspond to the velocity of the catalytic cycle of SERCA. Interestingly, the turnover rate, ATP c...

Ngày tải lên: 14/02/2014, 14:20

... [13]. These studies indicate that there is a controversy in the literature about the impor- tance of the CagL RGD-motif in T4SS functions and host cell signalling. Another unsolved question is the proposed ... numerous studies indicate that structural components of the T4SS but not CagA itself were required for the induction of pro-inflammatory signalling, including the act...

Ngày tải lên: 14/02/2014, 19:20