Báo cáo khoa học: Mitochondrial transcription factor A overexpression and base excision repair deficiency in the inner ear of rats with D-galactose-induced aging pdf
Báo cáo khoa học: Mitochondrial transcription factor A overexpression and base excision repair deficiency in the inner ear of rats with D-galactose-induced aging pdf
... accelerated aging of d-Gal-treated animals [21,22]. These charac- teristics resemble those of the natural aging process in humans and other animals. As the inner ear tissue is unacquirable during ... study in mice also reported that the capacity to repair oxidative DNA damage in various brain regions during aging was altered in an age-dependent manner, and in...
Ngày tải lên: 14/03/2014, 23:20
Báo cáo khoa học: Tumor necrosis factor-a converting enzyme is processed by proprotein-convertases to its mature form which is degraded upon phorbol ester stimulation pptx
... disintegrin and metalloproteinase) family of type I membrane proteins and mediates the ectodomain shedding of various membrane- anchored signaling and adhesion proteins. TACE is syn- thesized as ... anti-TACE, and a polyclonal rabbit antibody against endogenous TACE (Chemikon International, Temecula, CA). Both antibodies are directed against the C-terminal part of the pr...
Ngày tải lên: 08/03/2014, 02:20
Báo cáo khoa học: Tumour necrosis factor-a attenuates insulin action on phosphoenolpyruvate carboxykinase gene expression and gluconeogenesis by altering the cellular localization of Foxa2 in HepG2 cells pptx
... in obesity-associated insulin resistance and diabetes, inhibit insulin signalling at several points in the signalling cascade. The liver is critical in maintaining cir- culating glucose levels and, in a ... on phosphoenolpyruvate carboxykinase gene expression and gluconeogenesis by altering the cellular localization of Foxa2 in HepG2 cells Amit K. Pandey, Vikash Bhard...
Ngày tải lên: 16/03/2014, 02:20
Báo cáo khoa học: Tumor necrosis factor-a-induced caspase-1 gene expression pptx
... processes, including cell death, cell growth, stress response and in ammation [2,3]. One of the major signaling pathways induced by TNF -a leads to the activation of transcription factor nuclear factor- jB ... stimulation of the human lung carcinoma cell line, A5 49, with TNF -a increases the expression of caspase-1 mRNA and pro- tein. The increase in caspas...
Ngày tải lên: 16/03/2014, 05:20
Báo cáo khoa học: Sp3 transcription factor is crucial for transcriptional activation of the human NOX4 gene doc
... for the treatment of fibrosis, cancers, and cardiovascular and metabolic diseases. Materials and methods Materials An antibody against Sp1 was purchased from Active Motif (Carlsbad, CA, USA). Antibodies ... for amplification of the NOX4 promoter were as follows: 5¢-AACAATCAGTCTAAAAGAGCTGTGTCTT CT-3¢ (forward for GC-box1), 5¢-CTCCAAAATACTGG CAAACATGTGAACAAT-3¢ (reverse for GC-box1)...
Ngày tải lên: 29/03/2014, 00:20
Báo cáo khoa học: Interaction between catalytically inactive calpain and calpastatin Evidence for its occurrence in stimulated cells docx
... calpast- atin into the cytosol without affecting Ca 2+ homeostasis. The fact that the changes in the calpain state are also observed under the latter conditions indicates that calpastatin availability ... observations indicating that calpain and calpast- atin can associate in a 1 : 1 molar ratio, regardless of the presence of Ca 2+ (unpublished work). In addition to...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Abortive translation caused by peptidyl-tRNA drop-off at NGG codons in the early coding region of mRNA potx
... 1 Plasmid constructs are derivatives of pDA3480 [9,10]. The 5¢ end of the transcript is 5¢-AAUUGUGAGCGGAUAACAAUUUCA- CCAGGUAAUAAAUU AAAUAAAAUUUAAAUAUG-3¢ for the gene variants that lack a functional ... a plasmid with +5 AGG in the test gene was combined in the MB01 strain with the plasmid pUBS520 that carries the tRNA Arg4 gene, or plasmid pArgUW with the tR...
Ngày tải lên: 30/03/2014, 20:20
Tài liệu Báo cáo khoa học: Mitochondrial chaperone tumour necrosis factor receptor-associated protein 1 protects cardiomyocytes from hypoxic injury by regulating mitochondrial permeability transition pore opening docx
... The targeting sequence of the siRNA against rat TRAP1 was 5¢-CAACAGAGATTGATCAA AT-3¢. A negative control adenovirus vector containing nonspecific siRNA was constructed in the same way (non- specific ... expression increases in cardiomyocytes, the role of that TRAP1 increase remains unclear. The question remains as to whether the hypoxia-induced TRAP1 increase is a protec...
Ngày tải lên: 16/02/2014, 14:20
Tài liệu Báo cáo khoa học: Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae ) role of protein kinase A in the mitochondrial targeting of CYP2E1 pdf
... mitochondrial targeting of CYP2E1 Naresh B. V. Sepuri, Sanjay Yadav, Hindupur K. Anandatheerthavarada and Narayan G. Avadhani Department of Animal Biology, School of Veterinary Medicine, University of ... erythromycin, was preincubated for 3 min. The reaction was initiated by the addition of 3 mm NADPH, and continued for 30 min at 37 °C in a shaking water bath. The r...
Ngày tải lên: 18/02/2014, 16:20
Báo cáo khoa học: Epidermal growth factor receptor-regulated miR-125a-5p – a metastatic inhibitor of lung cancer potx
... against Akt was from BioVision, Inc. (CA, USA), and antibody against phospho-Akt was from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Western blot analysis To examine the in uence of ... miR-12 5a- 5p expression in a subset of human lung cancers To gain further insights into the role of miR-12 5a- 5p in lung carcinogenesis and to examine the clinical rele- van...
Ngày tải lên: 07/03/2014, 00:20