Báo cáo khoa học: Enzymatic features of the glucose metabolism in tumor cells ppt

... significance of the Warburg effect. We conclude that, besides the expression level of the meta- bolic enzymes involved in the glucose metabolism of tumor cells, the unique tumor- specific pattern of isozymes ... is capable of reducing the level of fructose 2,6-P 2 inde- pendent of the phosphorylation state of iPFK-2. Reducing the level of fructose 2,6...

Ngày tải lên: 14/03/2014, 23:20

... The starting points of the trun- cated SpsB and the self-cleavage product of SpsB are indicated in the SpsB sequence. The prediction of the transmembrane segment (TMS) was carried out using the PORTER ... preprotein and the lower one to the mature protein (predicted MW = 22.5 kDa). As shown in Fig. 1A, the substrate remained unprocessed in the absence of t...

Ngày tải lên: 07/03/2014, 01:20

... system of this species are reflected in the phylogeny of the hemoglobin amino-acid sequences, which are intermediate between those of other fish of the family Nototheniidae and of species of the more ... oxygen-binding properties, taking the ecological constraints under con- sideration. In view of the role of temperature in modifying the oxygenation-deoxygena...

Ngày tải lên: 08/03/2014, 08:20

... preventing binding of oxygen. We analyzed the azide inhibition and determined the inhibition constants for sodium azide, in order to see the effect of the mutation (L559A) on the binding properties ... stability of the trinuclear site is decreased, the binding of azide to the trinuclear site may be decreased. The depletion of the T2 copper and chloride ion may...

Ngày tải lên: 16/03/2014, 00:20

... [5]. Furthermore, a chromosomal point mutatio n in srfA-B, changing a D to A in the His-motif (italic) of the corresponding Asp domain of the surfactin synthetase was found to abolish surfactin production ... bound in the binding poc ket o f a nother e nzyme molecule within the active homomultimer. This could explain the distance in sequence homology with NRPSs C-domai...

Ngày tải lên: 17/03/2014, 11:20

... ethylene signalling converge at the level of EIN3 protein stabil- ity [42]. Role of sucrose splitting enzymes Sucrose is one of the most widespread disaccharides in nature. In higher plants it represents the ... development or an indirect effect via glucose signalling (Fig. 1). Intriguingly, the nuclear Fig. 1. A hypothetical sugar–antioxidant network in plant cells. G...

Ngày tải lên: 15/03/2014, 11:20

... alteration of the isoalloxazine microen- vironment induced by the binding of the NMN group. The small structural differences in the nicotinamide rings of NADP + , NADPO and thio-NADP + resulted in small ... exclude the formation of the species modified in position 3 of the nicotinamide ring because of the poor reactivity of this position towards nucleophil...

Ngày tải lên: 16/03/2014, 11:20

... excluded all residues involved in the binding of the metal cofactor. ApeSODs in different forms were superimposed with the least square fit of Ca atoms of the residues in the range 10–200. ApeSOD ... focus on the coordination of the metal cofactor in the active site as well as the changes it experiences in response to different metal cofactors. Finally, by c...

Ngày tải lên: 14/02/2014, 22:20

... [35] may not be considered in the light of the results obtained in the present study. Rather, the involvement of manganese in the in vivo assembly of the metallocofactor of C. ammoniagenes R2F is ... of protein uti- lizing the principle of protein-dye binding. Anal Bio- chem 72, 248–254. 48 Laemmli UK (1970) Cleavage of structural proteins during the asse...

Ngày tải lên: 15/02/2014, 01:20

... FEBS 3759 Introduction C-peptide is derived from most of the proinsulin seg- ment in between the B and A chains of insulin [1] and has an important structural role in the proper folding and disulfide ... distribu- tion of 100 l M proinsulin C-peptide in the presence of divalent Ca 2+ and Mg 2+ ions under native conditions (B), and of 100 l M C-peptide in the presenc...

Ngày tải lên: 18/02/2014, 04:20