Báo cáo khoa học: Enzymatic characterization and molecular m

Báo cáo khoa học: Enzymatic characterization and molecular modeling of an evolutionarily interesting fungal b-N-acetylhexosaminidase pot

... Department of Structure and Function of Proteins, Institute of Nanobiology and Structural Biology of GCRC, Academy of Sciences of the Czech Republic and Faculty of Sciences of the University of South ... hydrolysis of N-formyl, N-glycolyl and N-propionyl derivatives of the standard N-acetyl substrate) than A. oryzae (2–5% hydrolysis). The cloning and sequencing...

Ngày tải lên : 14/03/2014, 23:20

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Tài liệu Báo cáo khoa học: Biochemical characterization and inhibitor discovery of shikimate dehydrogenase from Helicobacter pylori docx

... s )1 and K m of 0.148 mm toward shikimate, k cat of 7.1 s )1 and K m of 0.182 mm toward NADP, k cat of 5.2 s )1 and K m of 2.9 mm toward NAD. The optimum pH of the enzyme activity is between 8.0 and ... catalytic properties of HpSDH and the effects of pH and temperature on HpSDH. The results show that HpSDH has a k cat of 7.7 ± 0.9 s )1 , K m of 0.148 ± 0.028...

Ngày tải lên : 19/02/2014, 05:20

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Tài liệu Báo cáo khoa học: Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L. docx

... human and pufferfish (torafugu and spotted green pufferfish) IL-10 sequences. Pufferfish and carp IL-10 genes, clustered together and distant from IL-20 and IL- 24, as recently determined from analysis ... control for the quantity and quality of cDNA. Semiquantitative analysis of RT–PCR products. Suspen- sions of Carp head kidney and liver cells were treated with 10 lgÆmL )1...

Ngày tải lên : 20/02/2014, 02:21

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Tài liệu Báo cáo khoa học: Physicochemical characterization and biological activity of a glycoglycerolipid from Mycoplasma fermentans ppt

... [23,30], and the bands at 1172, 1085, and 1038 cm )1 assigned to glucose ring vibrations [31]. As no additional bands in the range of the amide vibrations centered at 1650 and 1550 cm )1 can be observed, ... crystalline phase transition for phosphatidylcholine, MfGl-II, and LPS Re. Fig. 3. Infrared spectrum in the spectral range 1800–900 cm )1 (A) and in the range of the negativ...

Ngày tải lên : 21/02/2014, 00:20

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Báo cáo khoa học: Isolation, characterization and expression analysis of a hypoxia-responsive glucose transporter gene from the grass carp, Ctenopharyngodon idellus potx

... GLUT3 (59–60%) and GLUT4 (55–59%) proteins, and moderate Fig. 1. Organization of the gcGLUT gene. (A) The 12 exons of the gcGLUT gene are shown as boxes. Filled and open boxes indicate translated and untranslated ... identiﬁcation of a hypoxia-responsive glucose transporter gene (gcGLUT) and the corresponding full-length cDNA from the grass carp. The gene spans  11 kb of...

Ngày tải lên : 17/03/2014, 03:20

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Báo cáo khoa học: Identiﬁcation and expression analysis of an IL-18 homologue and its alternatively spliced form in rainbow trout (Oncorhynchus mykiss) doc

... genomic organization to the human IL-18 gene, consisting of six exons and ﬁve introns. The Fig. 2. Comparison of the genomic organization of IL-18 and IL-1b genes in human, rainbow trout and the ... twice with an equal volume of phenol/chloroform (24 : 1, v/v) and once with chloroform. Genomic DNA was precipitated with two volumes of cold ethanol, washed with 70% (v/v) eth...

Ngày tải lên : 16/03/2014, 16:20

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Báo cáo khoa học: Kinetic studies and molecular modelling attribute a crucial role in the speciﬁcity and stereoselectivity of penicillin acylase to the pair ArgA145-ArgB263 pdf

... structure [19] give evidence of polar environment around ArgB263. It consists of O atoms of the main chain CO groups of LeuB387 and TrpB240, Od 1 atom of AsnB241 and Oc atom of SerB386 and is expected to ... R.A., van Rantwijk, F., van Langen, L.M., Wegman, M.A., Cao, L. & Janssen, M.H.A. (2001) Biocatalysis and biocatalyst in the synthesis of b-lactam antibiotics. I...

Ngày tải lên : 16/03/2014, 16:20

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Tài liệu Báo cáo khoa học: Helix mobility and recognition function of the rat thyroid transcription factor 1 homeodomain – hints from 15N-NMR relaxation studies pdf

... 286 ± 0.5 K and at 11.7 T on a Bruker (Karlsruhe, Germany) Avance500 spectrometer, operating at 500.13 MHz and 50.68 MHz for 1 H and 15 N, respectively. The longitudinal (R 1 ) and trans- verse ... helix I, Ser27 and Leu34 in helix II, and Lys46 and Gln50 in the N-terminal portion of helix III. For an isotropically tumbling globular molecule, in the absence of internal mot...

Ngày tải lên : 18/02/2014, 16:20

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Tài liệu Báo cáo khoa học: The antibacterial and antifungal properties of trappin-2 (pre-elaﬁn) do not depend on its protease inhibitory function pptx

... nature of the molecule, as assessed from the salt and heparin dependence of the antimicrobial and antifungal effects. Results Antimicrobial effects of recombinant wild-type trappin-2 and trappin-2 ... PAGE gel and analyzed by western blotting using polyclonal anti-trappin-2 sera. The molecular masses of the protein standards are shown on the left. Antibacterial and antif...

Ngày tải lên : 18/02/2014, 17:20

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Tài liệu Báo cáo khoa học: Receptor association and tyrosine phosphorylation of S6 kinases pdf

... Stephens 7 , Andrew West 7 and Ivan T. Gout 2,3 1 Ludwig Institute for Cancer Research, London, UK 2 Department of Biochemistry and Molecular Biology, University College London, UK 3 The Institute of Molecular ... domains and have a similar mode of activation [1]. There are two isoforms of S6 kinase, S6K1 and 2. Both have highly homologous kinase and kinase extension dom...

Ngày tải lên : 19/02/2014, 07:20

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