Báo cáo khoa học: Shewasin A, an active pepsin homolog from the bacterium Shewanella amazonensis pptx

... sequence. The Asp of the active site motif from the N-terminal domain (marked with an aster- isk) was mutated to an Ala to generate the active site mutant shewasin A_(D37A). Although it displays the ... Purification and analysis of recombinant shewasin A active site mutant. The active site mutant shewasin A_(D37A) was expressed in E. coli, purified according to...

Ngày tải lên: 14/03/2014, 22:20

... (Hartmann Analytic, Braunschweig, Germany) was added. The supernatants were extracted with XAD16 resin after an additional 2 days of growth. The dried eluate was dissolved in 10% methanol and analyzed ... carbonyl carbon of the serine and the d-CH 2 of the hOrn, which constitutes the DKP. Therefore, putting all these long-range connections together, we established a structure fo...

Ngày tải lên: 16/02/2014, 09:20

... reveals two transitions, the first in the pH range pH 7–5, and the second in the pH range 5–3. The observed transitions in the aci- dic pH range are likely to be the result of decreasing the net negative ... The changes in molar ellipticity followed at 222 nm over the pH range 7.0–10.0 are not significant, and no changes were seen in the absorbance at 262 nm in the pH ra...

Ngày tải lên: 16/03/2014, 13:20

... 1.5% and the lysate was sonicated and centrifuged. The supernatant was incubated with 2% Triton-X-100 and 250 lL of glutathione-sepharose beads. The recombinant protein was eluted from the beads ... efficiency. Acknowledgements We thank all members of the laboratory for their sug- gestions and critical reading of the manuscript. This work was supported by grants from the DFG...

Ngày tải lên: 23/03/2014, 04:20

... extract and 20 lgÆmL )1 gentamicin (Centafarm Services, Etten- Leur, the Netherlands). The human small cell lung cancer (GLC4) cell line and GLC4 cell line transfected the human multidrug resistance ... pur- chased from Abic Ltd (Netanya, Israel). JC-1, DCF and Fluo-3 were purchased from Molecular probes Europe BV (Leiden, the Netherlands). Cell culture Human-derived renal proxima...

Ngày tải lên: 30/03/2014, 08:20

... likely due to the increased distance between the catalytic base and the 5¢-OH of either dThd or dCyd. These results favour the reaction-mechanistic hypothesis’ emphasis on arginine and glutamate ... and the information from the structures of HSV1-TK we suggest that the network of hydrogen bonds is disrupted, and this gives rise to an increase in K m . Also, the polarity is...

Ngày tải lên: 19/02/2014, 02:20

... shows the positions of nucleosomes on the MMTV promoter [6], the transcriptional start site and the cleavage sites for SacI(S),HinfI (H) and BamHI (B). (B) Radioactivity scans from selected lanes. Ó ... in hormone-induced transcription ( 50% of full induction when added early, compare lanes 3, 4 and 11, 12). Neither of these effects depended on the TSA concentration over the ra...

Ngày tải lên: 19/02/2014, 12:20

... reactions, and the synthesis of polyamines, antioxidants, and cofactors [1]. As there are remarkable differences in trans-sulfuration pathways between organisms, these pathways, and in Keywords amoebiasis; ... additional bands were found (Fig. 2A, lane 1, open arrowheads). Incubation of EhMGLs with Met or without substrates did not result in the appearance of these bands (Fig. 2A, lanes...

Ngày tải lên: 07/03/2014, 05:20

... proteins into the host, and transcriptomic and proteomic approaches, during the different life stages of the para- site, have been applied to investigate the importance of these proteins for the parasite–host ... of the Ca 2+ -bind- ing loops. The two EF-hands are covalently bonded via the B ⁄ C linker, which connects the exiting helix (B) of the first EF-hand to the first...

Ngày tải lên: 15/03/2014, 00:20

... 4 C,D, lanes 2 and 3 in both panels). On the other hand, me thyl green did not affect the f ormation of the complexes (lanes 4 and 5 ). The insensitivity of nRLjunRP and rRLjunRP to the major ... outin the presence of 1 ng of rad iolab elled Jun-25 and 100 lg(lanes1,3and5)and150lg (lanes 2, 4 and 6) o f nuclear extracts. Lanes 1 and 2 r e present EM SA performed with nRNE-d, lanes...

Ngày tải lên: 16/03/2014, 18:20