Báo cáo khoa học: Enzymatic toxins from snake venom: structural characterization and mechanism of catalysis ppt

... number of pro- tein toxins have been purified and characterized from snake venoms [1,2] and snake venoms typically contain from 30 to over 100 protein toxins. Some of these pro- teins exhibit enzymatic ... the Enzymatic toxins from snake venom T. S. Kang et al. 4562 FEBS Journal 278 (2011) 4544–4576 ª 2011 The Authors Journal compilation ª 2011 FEBS REVIEW ARTICLE...

Ngày tải lên: 14/03/2014, 22:20

... Belgium) and all the solvents used for HPLC were of HPLC grade (Biosolve, Valkenswaard, the Netherlands). Chemical synthesis and purification of AThDP and AThTP AThTP was synthesized by modification of ... thought and may have physio- logical roles both in prokaryotes and eukaryotes. Results Chemical synthesis and purification of AThDP and AThTP We have previously shown tha...

Ngày tải lên: 07/03/2014, 01:20

... (Asn) and serine (Ser) [4,12]. In the active center of silicatein, the hydroxyl group of Ser26 and the imidazole of His165 (catalytic diad) have been shown to play key roles in the condensation of ... development of a functional model that contrib- utes to understanding of the growth of the siliceous spicules which apparently lack a template or matrix. The simultaneous relea...

Ngày tải lên: 18/02/2014, 16:20

... CD samples. Spectra were recorded from 260 to 190 nm at a speed of 50 nmÆmin )1 , band-width of 1.0 nm, and a resolution of 0.1 nm in the temperature range between 10 °Cand70°C, after preincubation ... identical (from residue number 3) to the sequence of the corresponding component of PH from A. calcoace- ticus NCIB 8250 (11/11 identity) [3] and very similar to that of th...

Ngày tải lên: 21/02/2014, 00:20

... is a soft ligand and therefore shows a preference for soft metals. More- over the structures of metallothioneins are not rigid and hence there is little selectivity concerning the size of the ... Table 1 and discussed in more detail below. Metal content of MT3 Initially, MT3 was isolated from human brain with a metal content of four Cu(I) and three Zn(II) per MT3 Fig. 1. Sch...

Ngày tải lên: 16/02/2014, 15:20

... surgical transections of ligaments and medial meniscectomies to explore the under- lying mechanisms of osteoarthritis. Cartilage and synovial fluid obtained from normal animals and those with osteoarthritis ... proteasome inhibition and TRAIL in osteoarthritic disease and chondrocyte apoptosis, and the hypoxic inhibition of cartilage and chondrocyte degeneration. Results...

Ngày tải lên: 18/02/2014, 14:20

... microscopy of thin-sectioned nuclei of cells and tissues indicated the existence of a continuous layer of proteins apposed to the inner membrane of the nuclear envelope, in the vast majority of cells ... spermatocytes and oocytes possibly reflects the distinct organization of the nuclei of these cells in general and chromatin in particular in germ cells. Indeed, alteratio...

Ngày tải lên: 19/02/2014, 02:20

... (Sigma-Aldrich). The composi- tion of the mobile phase (flow rate of 1 mLÆmin )1 ) was 80% of 0.1% TFA in water and 20% of 0.1% TFA in 40% acetonitrile. Excitation and emission wavelengths of the fluorescence ... fluorescence detector were set at 450 and 525 nm, respectively. FAD and FMN standards were used to iden- tify the nature of the flavinic cofactor. UV-visible spectra...

Ngày tải lên: 19/02/2014, 06:20

... molecular analysis of specific factors involved should provide a clearer understanding of the function of the Gua– RPL4 interaction and its significance in a more inclu- sive picture of the ribosome ... component of 60S ribosome large subunit. The ATPase activity of Gua is important for this interaction and is also necessary for the func- tion of Gua in the production of bot...

Ngày tải lên: 20/02/2014, 01:20

... selection of natural questions. The articles varied in topic, degree of formality and the amount of details; from ”Horror film” and ”Christ- mas worldwide” to ”G-Man (Half-Life)” and ”His- tory of London”. ... were grouped into groups of 5. Each HIT consisted of 5 assignments, and a $0.05 reward was offered for each HIT. QRepHIT required the least amount of design and...

Ngày tải lên: 20/02/2014, 09:20