... less sensitive to the pres-ence of the anion. These residues also modulate the conformation of unfol-ded cytochrome c, influencing its spin state and the coordination to the prosthetic group.AbbreviationCT, ... effect of Cl–reflects the different affinities of the two anionsfor the protein [11,17].We also monitored the effect of sulfate on the perchlorate-induced A-state. As shown in Fig. 6, addi-tion of ... that the variants undergo very fast optical (and thus structural)changes within the dead time of the stopped-flowapparatus. In the case of the K13N mutant, this hypo-thesis finds support from the...