plasmids and vectors - Instructor Supplement to pGlo Bacterial Transformation

plasmids and vectors - Instructor Supplement to pGlo Bacterial Transformation

plasmids and vectors - Instructor Supplement to pGlo Bacterial Transformation

. the non-essential parts of lambda  Can now insert large pieces of DNA (~ 20 kb) COS Lysis Head Tail Replication ori Plasmids and Vectors Instructor Supplement to pGlo Bacterial Transformation . packaging into phage particle Cosmids Other Vectors  BACs (Bacterial artificial chromosomes)  Large low copy number plasmids (have ori and selectable marker)  Can be electroporated into...

Ngày tải lên: 13/03/2014, 18:46

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Workplace Writing Skills Developing Clarity and Accuracy A Resource to Supplement Existing Published Materials doc

Workplace Writing Skills Developing Clarity and Accuracy A Resource to Supplement Existing Published Materials doc

. follow-up step and date. Be as pleasant and positive as possible.  I want to work with you.  I appreciate your attention to this and I expect  I would like you to  I don’t want to cancel,. need to be limited to five points per category. Lists need to be easy to understand and remember. If there are more than five items, create more categories, or divide the information into smaller....

Ngày tải lên: 19/03/2014, 08:20

52 508 0
Strings and Vectors

Strings and Vectors

... Addison-Wesley Chapter 8 Strings and Vectors Slide 8- 20 Copyright © 2007 Pearson Education, Inc. Publishing as Pearson Addison-Wesley C-strings as Arguments and Parameters  C-string variables ... 26 Copyright © 2007 Pearson Education, Inc. Publishing as Pearson Addison-Wesley getline and Files  C-string input and output work the same way with file streams  Replace cin with th...

Ngày tải lên: 12/09/2012, 22:51

92 410 1
The Scientist and Engineer's Guide to Digital Signal Processing Second Edition

The Scientist and Engineer's Guide to Digital Signal Processing Second Edition

. publisher. ISBN 0-9 66017 6-7 -6 hardcover ISBN 0-9 66017 6-4 -1 paperback ISBN 0-9 66017 6-6 -8 electronic LCCN 9 7-8 0293 California Technical Publishing P.O. Box 502407 San Diego, CA 9215 0-2 407 To contact. conform to any industry standard, or are suitable for any application. The author, publisher, and distributors disclaim all liability and responsibility to any person or entity with resp...

Ngày tải lên: 13/09/2012, 10:21

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Báo cáo y học: "ntravenous transplantation of allogeneic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head"

Báo cáo y học: "ntravenous transplantation of allogeneic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head"

. TNF-α, IFN-α, IL-4, and IL-10 and modulate Treg cells to reduce the incidence of graft-versus-host disease. In addition, inhibition or restriction of these inflamma- tory mediators also alleviates. chemotatic factors including interleukin-8 (IL-8), monocyte chemoattractant pro- tein (MCP-1), stromal cell-derived factor (SDF-1) and tumor necrosis factor (TNF) are produced. Mean- while, the....

Ngày tải lên: 25/10/2012, 11:18

10 584 0
 Báo cáo y học: " Carotid Intima-media thickness in childhood and adolescent obesity relations to abdominal obesity, high triglyceride level and insulin resistance"

Báo cáo y học: " Carotid Intima-media thickness in childhood and adolescent obesity relations to abdominal obesity, high triglyceride level and insulin resistance"

. insulin; HOMA-IR = homoeostasis model assessment- insulin resistance; TG = triglyceride; TC = total cholesterol; HDL-C = high-density lipoprotein- cholesterol; LDL-C = low-density lipoprotein-cholesterol;. interleukins and other inflammatory cyto- kines by human adipose tissue is enhanced in obesity and pri- marily due to the nonfat cells. Vitam Horm.2006; 74:443 -4 77 6. Suh M, Lee JY, Ahn SV...

Ngày tải lên: 25/10/2012, 11:40

6 478 0
An investigation into the reality of teaching and learning speaking skills to the 2nd year non-major english students at pre-intermediate level of proficiency at hanoi university of industry

An investigation into the reality of teaching and learning speaking skills to the 2nd year non-major english students at pre-intermediate level of proficiency at hanoi university of industry

. analysis, and the findings and discussions. Chapter 3 offers some possible suggestions to improve the teaching and learning English speaking to 2 nd year non-major English students. Part three - the. Provide some necessary vocabulary and structures related to the topic. E. Discuss with students to lead them to the topic. F. Brainstorm the topic. G. Others (please specify) 11. What do you do....

Ngày tải lên: 07/11/2012, 14:50

20 2,4K 27
Exergoeconomic performance optimization of an endoreversible intercooled regenerative Brayton combined heat and power plant coupled to variable-temperature heat reservoirs

Exergoeconomic performance optimization of an endoreversible intercooled regenerative Brayton combined heat and power plant coupled to variable-temperature heat reservoirs

. regenerative Brayton closed- cycle coupled to variable-temperature heat reservoirs is shown in Figure 1. Process 1-2 and 3-4 are isentropic adiabatic compression processes in the low- and high-pressure. paper is to optimize the heat conductance allocation among the hot-, cold- and consumer-side heat exchangers, the intercooler and the regenerator, the intercooling pressure ratio and the total...

Ngày tải lên: 05/09/2013, 14:59

16 606 0
Sustaining an Interest in Learning English and Increasing the Motivation to Learn English

Sustaining an Interest in Learning English and Increasing the Motivation to Learn English

. limited and non-conducive to learning the target language, teachers need to find creative ways to teach the language and increase the student's motivation to learn the language and to eventually. students for the standardized examinations and for life-long language skills. One solution is to develop a continuous program which includes an integrated in- class and out-of-class language ac...

Ngày tải lên: 06/09/2013, 10:10

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Have and have got & Use to (do)

Have and have got & Use to (do)

. Have you got any money? - I haven’t got any money Do you have any momey? - I don’t have any money Have you any money? - I haven’t any money (ít dùng) Has she got a car? - She hasn’t got a car . usually have a sandwich for my lunch. (have = eat - not “have got”) Buổi trưa tôi thường ăn bánh sandwich. (ở đây have có nghĩa là ăn, không mang nghĩa sở hữu) Nhưng I’ve got some sandwichs.....

Ngày tải lên: 23/10/2013, 15:15

6 540 1
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