... 1 Proteins involved in mitotic spindle checkpoint regulationProtein Proposed function in the spindle assembly checkpointMad1 Coiled-coil protein, binds to Mad2 and recruits Mad2 to kinetochoresphosphorylated ... to the spin-96 I. HoffmannFig. 1 Functions of protein kinases in the spindle checkpoint. Attachment of chromo-somes to the mitotic spindle is monitored by the spindle checkpoint. Sensing mechanismsmay ... checkpointAurora B Protein kinase, binds to INCENP, other substrates: CENP-A, Rec8,vimentin, desmin, the kinesin MCAK, histone H3Aurora C Protein kinase, binds to INCENP and Aurora BMAPK Protein kinaseRod...