Báo cáo Y học: Identification of residues critical for activity of the wound-induced leucine aminopeptidase (LAP-A) of tomato pptx
Báo cáo Y học: Identification of residues in the PXR ligand binding domain critical for species specific and constitutive activation docx
... Q404Hmutant, where the basal activity decreased by more than50% (Fig. 4). The basal activity of H407Q and Q404A on the other hand remained close to wild-type levels. The structural reasons for the observed ... closely with the crystalstructure. There are other parts of the model that do notcorrelate with the crystal structure, in particular the regionFig. 4. A diagram of basal transcriptional activity ... effect of PCN on Q404H should be viewed inperspective of the basal activity. The full activation of the mutant is similar in level to the wild-type in absolute terms,but as the basal activity is...
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Báo cáo Y học: Identification of residues critical for activity of the wound-induced leucine aminopeptidase (LAP-A) of tomato pptx
... 269) Ó FEBS 2002 Identification of residues critical for activity of the wound-induced leucine aminopeptidase (LAP-A) of tomato Yong-Qiang Gu* and Linda L. WallingDepartment of Botany and Plant Sciences, ... and the carbonyl oxygen of the P1 residue, Zn1 is bound by the two carboxyl oxygens of Asp25 5 (Asp347) and a car boxyl ox ygen of Glu334(Glu429). T he interact ions of Zn1 and the carboxyl oxygen of ... (Asp366), the carbonyl oxygen of the P1residue with the side-chain amine of Lys262 (Lys354), and the backbone amino group of the P 1¢ residue with the backbone carbonyl oxygen of Leu360 (Leu455).There...
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Tài liệu Báo cáo Y học: Identification and characterization of a mammalian 14-kDa phosphohistidine phosphatase pdf
... was only 2% of thatat pH 7.5. No activity was detected at pH 9.0. The pH-dependence of the recombinant human enzyme wasthus similar to that of the porcine liver enzyme. The specificity of the recombinant ... considerationalso for other combinations of phosphatases and interme-diary phosphorylated enzymes. Therefore, kinetic data on the dephosphorylation of autophosphorylated ATP-citratelyase in the paper ... A˚keEngstro¨m at the Peptide Synthesis and Analysis Laboratory of the Department of Medical Biochemistry and Microbio-logy, Uppsala University. The SP6 primer 5¢-ATT TAGGTG ACA CTA TAG-3¢ and the three...
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Tài liệu Báo cáo Y học: Identification of a set of genes involved in the biosynthesis of the aminonucleoside moiety of antibiotic A201A from Streptomyces capreolus pdf
... proteinsimplicated in the biosynthesis of the aminonucleoside moiety of A201A. Here we report the sequencing of a total of 6946 bp, which include the ata genes most likely implicatedin the biosynthesis of this ... GDP-D-mannose and other hexosedehydratases (data not shown) (Fig. 3). A variety of theseenzymes synthesize 4-keto-6-deoxy-GDP-D-mannose, a keyintermediate in the biosynthesis of many deoxyhexoses ... This domain promotes the binding of the acyl-CoA initiation unit to the ketosynthetasedomain of the PKSs for polyketide biosynthesis [35].Therefore, AtaPKS1 could be an acyltransferase, whichshould...
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Tài liệu Báo cáo Y học: Identification of syntaxin-1A sites of phosphorylation by casein kinase I and casein kinase II ppt
... structures [14]. Therefore, we examined whetherCKIa was capable of phosphorylating syntaxin-1A.Figure 1 shows that the cytoplasmic tail of syntaxin-1A (residues 1–265) is ph osphorylated by recombinant ... from the two constructs.Similarly, we identified the residue(s) of syntaxin-1Aphosphorylated by CKII. Phosphorylated forms of syn-taxin-1A (1–190 and 1–265) were digested with trypsin and the ... secretion is the key s tep in synaptic transmissionand is the basis of intercellular communication in the nervous system. Synaptic vesicle exocytosis is regulatedby Ca2+and by a large number of proteins...
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Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx
... of the stationary phase the rate of synthesis of heat-shock proteins increases considerably,but only transiently [2,18]. Similarly, we observed that the steady-state amount of GroEL remains ... the deletion of the UP12 encoding gene increased the sensitivity of the mutant to CCCP (Fig. 6), it did not haveany detectable effects on the sensitivity to mitomycin C,unlike in the case of ... mMphenylmethanesulfonyl fluoride. Cells were thendisrupted by treatment with lysozyme (1 mgÆmL)1)onice for 10 min followed by a single rapid cycle of freeze andthaw at 4 °C. The entire cell lysate...
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Báo cáo Y học: Identification of the 19S regulatory particle subunits from the rice 26S proteasome potx
... relationship between N-acetylation and the chymotrypsin-like activity of 20S proteasome. In the present s tudy, we detec ted the N-acetylation of OsRpt6 in the rice RP complex by MS. Other N-terminal modifica-tions ... masses of the RP subunits were between32 000 and 110 000 D a. Identification of the rice RP subunit by ESI-Q-TOFmass spectrometryPrior to identification of the RP subunits by ESI-Q-TOFMS, we ... the N-terminus of 80–90% of proteins in the cell is acetylated. Recently, Kimura et al.([32]; Kimura, Y. , Yokohama City University, KiharaInstitute for Biological Research/Graduate School of Integrated...
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Báo cáo Y học: Identification and characterization of a new gene from Variovorax paradoxus Iso1 encoding N -acyl-D-amino acid amidohydrolase responsible for D-amino acid production pdf
... assayed for enzyme activity and the active fractions were further analysed byWestern blotting.Enzyme activity assay The standard reaction mixture (0.5 mL) for the determin-ation of N-D-AAase activity ... possibly a metal-dependent enzyme.Substrate specificity analysisTo study the substrate specificity of the N-D-AAase protein, the activity of the enzyme against N-acyl-D-orL-amino acidsand otherD-amino ... resulted in a 60% loss of activity (Fig. 5B).Above 55 °C, activity decreased rapidly reflecting the instability of the enzyme at higher temperatures. The optimal pH for enzyme activity was pH 7.5 (Fig....
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Báo cáo Y học: Identification of novel membrane proteins by searching for patterns in hydropathy profiles potx
... designed for the acetylcholine (ACh)and glycine receptors of the cys-loop receptor superfamily. The key feature was a closely spaced triplet of hydropathypeaks bracketed by deep valleys. When ... database. By subtype, 90% of the kainate receptors in the database were detected, but only36% of the NMDA receptors, and 1% of the AMPAreceptors. Examination of the AMPA receptor hydropathyprofiles ... (A) The hydropathy profile of a rat dopaminesymporter reveals a pair of peaks followed by a deep valley, then acluster of nine peaks. The peak, base and valley threshold levels usedby the search...
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Báo cáo Y học: Identification of mammalian-type transglutaminase in Physarum polycephalum Evidence from the cDNA sequence and involvement of GTP in the regulation of transamidating activity potx
... nucleotides inhibit the enzymatic activity of PpTGase might be similar to that bywhich they inhibit the enzymatic activity of TGase 4.Hydrolysing activity of GTP was also found in the purified PpTGase ... presence of the nucleotides. GTP and ATPclearly inhibited the activity, while GDP showed weakinhibition. GMP did not block the enzymatic activity. Next, GTP-hydrolysing activity of the purified ... CaCl2andadded to the reaction mixture. The TGase activity wasmeasured by the microtiter assay.Assays for GTP hydrolysis by TGase 2, TGase 3,and PpTGaseGTP-hydrolysing activity was measured...
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