Báo cáo Y học: Identification of residues critical for activity of the wound-induced leucine aminopeptidase (LAP-A) of tomato pptx

... Q404H mutant, where the basal activity decreased by more than 50% (Fig. 4). The basal activity of H407Q and Q404A on the other hand remained close to wild-type levels. The structural reasons for the observed ... closely with the crystal structure. There are other parts of the model that do not correlate with the crystal structure, in particular the region Fig. 4. A...

Ngày tải lên: 08/03/2014, 16:20

... 269) Ó FEBS 2002 Identification of residues critical for activity of the wound-induced leucine aminopeptidase (LAP-A) of tomato Yong-Qiang Gu* and Linda L. Walling Department of Botany and Plant Sciences, ... and the carbonyl oxygen of the P1 residue, Zn1 is bound by the two carboxyl oxygens of Asp25 5 (Asp347) and a car boxyl ox ygen of Glu334 (Glu4...

Ngày tải lên: 08/03/2014, 23:20

... was only 2% of that at pH 7.5. No activity was detected at pH 9.0. The pH-dependence of the recombinant human enzyme was thus similar to that of the porcine liver enzyme. The specificity of the recombinant ... consideration also for other combinations of phosphatases and interme- diary phosphorylated enzymes. Therefore, kinetic data on the dephosphorylation of autop...

Ngày tải lên: 21/02/2014, 01:21

... proteins implicated in the biosynthesis of the aminonucleoside moiety of A201A. Here we report the sequencing of a total of 6946 bp, which include the ata genes most likely implicated in the biosynthesis of this ... GDP- D -mannose and other hexose dehydratases (data not shown) (Fig. 3). A variety of these enzymes synthesize 4-keto-6-deoxy-GDP- D -mannose, a key intermed...

Ngày tải lên: 21/02/2014, 01:21

... structures [14]. Therefore, we examined whether CKIa was capable of phosphorylating syntaxin-1A. Figure 1 shows that the cytoplasmic tail of syntaxin-1A (residues 1–265) is ph osphorylated by recombinant ... from the two constructs. Similarly, we identified the residue(s) of syntaxin-1A phosphorylated by CKII. Phosphorylated forms of syn- taxin-1A (1–190 and 1–265) were digeste...

Ngày tải lên: 22/02/2014, 04:20

... of the stationary phase the rate of synthesis of heat-shock proteins increases considerably, but only transiently [2,18]. Similarly, we observed that the steady-state amount of GroEL remains ... the deletion of the UP12 encoding gene increased the sensitivity of the mutant to CCCP (Fig. 6), it did not have any detectable effects on the sensitivity to mitomycin C, u...

Ngày tải lên: 22/02/2014, 07:20

... relationship between N-acetylation and the chymotrypsin-like activity of 20S proteasome. In the present s tudy, we detec ted the N-acetylation of OsRpt6 in the rice RP complex by MS. Other N-terminal modifica- tions ... masses of the RP subunits were between 32 000 and 110 000 D a. Identification of the rice RP subunit by ESI-Q-TOF mass spectrometry Prior to identificati...

Ngày tải lên: 08/03/2014, 16:20

... assayed for enzyme activity and the active fractions were further analysed by Western blotting. Enzyme activity assay The standard reaction mixture (0.5 mL) for the determin- ation of N-D-AAase activity ... possibly a metal- dependent enzyme. Substrate specificity analysis To study the substrate specificity of the N-D-AAase protein, the activity of the enzyme aga...

Ngày tải lên: 08/03/2014, 16:20

... designed for the acetylcholine (ACh) and glycine receptors of the cys-loop receptor superfamily. The key feature was a closely spaced triplet of hydropathy peaks bracketed by deep valleys. When ... database. By subtype, 90% of the kainate receptors in the database were detected, but only 36% of the NMDA receptors, and 1% of the AMPA receptors. Examination of the...

Ngày tải lên: 17/03/2014, 23:20

... nucleotides inhibit the enzymatic activity of PpTGase might be similar to that by which they inhibit the enzymatic activity of TGase 4. Hydrolysing activity of GTP was also found in the purified PpTGase ... presence of the nucleotides. GTP and ATP clearly inhibited the activity, while GDP showed weak inhibition. GMP did not block the enzymatic activity. Next, GTP-h...

Ngày tải lên: 17/03/2014, 23:20