Báo cáo Y học: Molecular mechanisms underlying SHP-1 gene expression potx

... employed the Fmoc (9-fluorenyl-methyloxycarbonyl) methodology [37, 38] in a fully automated multiple synthesizer (Syro II from Multi Syntech Germany). As coupling agent 2-(1H-Benzotriazole-1-yl)-1,1,3,3-tetramethylu- roniumhexafluoro-phosphate ... impact on the gene expression patterns, when analyzing the efficacy and the genetic consequences of targeted gene therapy in vitro (and...

Ngày tải lên: 03/11/2012, 11:44

... Th1-type cytokine production in coronary atherosclerosis [32]. Finally, IL-17 which is produced by activated CD4+ T-cells acts synergistically to elicit further pro- duction of pro-inflammatory ... limited by the potential for adverse effects such as hypertension, dyslipidemia, hyperhomocysteinemia, and renal and hepatic toxicity. Thus preventive measures may be required during therapy...

Ngày tải lên: 25/10/2012, 11:40

... pseudotypes displaying functional HCV glycoproteins. These pseudotypes turned out to provide a robust model system for the study of viral entry [10, 11]. HCV pseudoparticle infectivity is pH-dependent ... replaced by the sequence encoding the neomycin phosphotransferase gene resulting in G418 resistance. Expression of the nonstructural proteins is directed by a heterologous encephalomy...

Ngày tải lên: 02/11/2012, 10:00

... endothelial lipase [1,2]. The primary function of LPL is triglyceride hydrolysis in triglyceride-rich lipoproteins, such as chylomicron and very low density lipoprotein (VLDL) particles, which ... domain Yoko Kobayashi, Toshiaki Nakajima and Ituro Inoue Division of Genetic Diagnosis, Institute of Medical Science, The University of Tokyo, Tokyo, Japan Lipoprotein lipase (LPL) plays a key role...

Ngày tải lên: 21/02/2014, 15:20

... phosphate, acetyl or methyl group), leading to a product that is distin- guishable by mass spectrometry or immunochemistry from the incoming molecular entity. The modification imposed by prolyl cis ⁄ ... activity by the immunophilin FKBP52. Immunity 12, 129–140. 80 Shen ZJ, Esnault S & Malter JS (2005) The peptidyl– prolyl isomerase Pin1 regulates the stability of granulo- cyte–macrophag...

Ngày tải lên: 07/03/2014, 05:20

... coupled to keyhole limpet haemocyanin via their endogenous (Rab31) or added (Rab32, Rab11A) cysteine residues, using 4-(N-maleimidomethyl) cyclohexane-1-carboxylate. Rabbits were immunized by intradermal ... W.A. (1996) Molecular cloning of two novel rab genes from human melano- cytes. Gene 174, 129±134. 26. Terryn, N., Anuntalabhochai, S., Van Montagu, M. & Inze  ,D. (1992) Analys i...

Ngày tải lên: 08/03/2014, 16:20

... as described by Trager and Jensen [8]. Cultures were synchronised by two rounds of sedimentation using gelatine. Briefly, cultures of approximately 5% parasitised erythrocytes (PE) were centrifuged, ... was performed with the GeneAmp ® 5700 system (PE Applied Biosystems) using the following cycle profile: 1 cycle at 48°C for 30 min, 1 cycle at 95°C for 10 min, 40 cycles at 95°C for...

Ngày tải lên: 02/11/2012, 10:14

... ATPase activity at either pH 7.4 or pH 6.4 (Fig. 3). At pH 7.4, 5m M azide inhibited ADP hydrolysis by  70% whereas ATP hydrolysis was inhibited by only 10%. Inhibition of ADP and ATP hydrolysis by azide ... ecto-ATPDase were affected differently by pH. Figure 2 shows that the pH–activity curves of ATP and ADP hydrolysis do not coincide. While maximal ATP hydrolysis activity was obtained in...

Ngày tải lên: 22/02/2014, 04:20

... 11b(OH)-androstendi- one were synthesized by CYP11B2 in comparison with CYP11B1 (Fig. 4C). It is noteworthy, that CYP11B2 displayed a higher enzymatic activity than CYP11B1 based on 11b-hydroxylase activity. These ... by means of an 11b-hydroxylation and an 18-hydroxylation/oxidation of 11-deoxycorticosterone. Thus, the proteins of the CYP11B subfamily catalysing the last biosynthetic steps a...

Ngày tải lên: 22/02/2014, 07:20

... carrying four acyl residues. GlcN residue A had unsubstituted hydroxyl group at C-1, and was mostly present in an a-pyranose form. Acyl 1 ,acyl 2 and acyl 3 residues had hydroxy or acyloxy groups ... 2-N-(3-O- acyl 4 -acyl 2 )-b-GlcN-(1–6)-2-N-acyl 1 )3-O-acyl 3 -GlcN where acyl 1 ,acyl 2 and acyl 3 are 3-hydroxyhexadecanoic or 3-hy- droxyoctadecanoic acids, acyl 4 is tetradecanoic or (minor)...

Ngày tải lên: 08/03/2014, 09:20