Báo cáo Y học: Puriﬁcation and characterization of novel kin

Tài liệu Báo cáo Y học: Puriﬁcation, characterization, cloning, and expression of the chicken liver ecto-ATP-diphosphohydrolase pot

... 7.4, 5m M azide inhibited ADP hydrolysis by  70% whereas ATP hydrolysis was inhibited by only 10%. Inhibition of ADP and ATP hydrolysis by azide was greater when the pH of assay solutions was 6.4. Azide inhibition ... 0.2 and 0.64 depending on the pH and the presence of NP-40; (d) Fig. 3. Inhibition of ATP and ADP hydrolysis activities of puriﬁed chicken liver ecto-ATPDase...

Ngày tải lên : 22/02/2014, 04:20

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Tài liệu Báo cáo Y học: Puriﬁcation, characterization, immunolocalization and structural analysis of the abundant cytoplasmic b-amylase from Calystegia sepium (hedge bindweed) rhizomes ppt

... residues of the C. sepium b-amylase (Cys83, Cys96, Cys209 and Cys344) are homologous to those found in soybean b-amylase (Cys82, Cys97, Cys208 and Cys343). On the analogy of the soybean b-amylase, ... method. Inhibition of the enzyme activity by glucose, maltose and cyclohexaamylose For the study of the enzyme inhibition by glucose, maltose and cyclohexaamylose b-amylase activ...

Ngày tải lên : 22/02/2014, 07:20

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Báo cáo Y học: Puriﬁcation and biochemical characterization of some of the properties of recombinant human kynureninase pptx

... regulation of enzyme activity in vivo and consequent channeling of substrate 3-hydroxykynurenine down the tryptophan– kynurenine metabolic pathway. The puriﬁcation of recombinant human kynureninase to ... Puriﬁcation and biochemical characterization of some of the properties of recombinant human kynureninase Harold A. Walsh and Nigel P. Botting School of Chemistry, U...

Ngày tải lên : 08/03/2014, 22:20

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Tài liệu Báo cáo khoa học: Puriﬁcation and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx

... CA, USA), and triﬂuoroacetic acid and acetonitrile used for HPLC were from Merck (Darms- tadt, Germany). Trypsin and tosyl phenylalanyl chloro- methyl ketone-treated chymotrypsin were from Sigma (St ... analyze the family of 20 structures (Table 3). Figure 3 shows an overlay of the backbone atoms for the 20 structures of conomarphin and l-Phe13-conomarphin (Protein Data Ba...

Ngày tải lên : 18/02/2014, 17:20

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Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of glutamate N-acetyltransferase involved in citrulline accumulation in wild watermelon doc

... transfer of the acetyl group from N-acetylornithine into glutamate yielding ornithine and N-acetylglutamate. Glutamate N-acetyltransferase therefore recycles the acetyl moiety of N-acetylornithine, ... acetyl- recycling pathway, both the ﬁrst- and the second-step enzymes, N-acetylglutamate synthase and N-acetylglutamate kinase, respectively, are inhibited by arginine. Glutamat...

Ngày tải lên : 20/02/2014, 03:20

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Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a sialic acid speciﬁc lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf

... lectin agglutinated only a limited range of erythrocytes. Out of 12 erythrocyte types tested the lectin could agglutinate only six erythrocyte types (Table 2). Our study on the sialoconjugates ... evaluation of the literature revealed that purification of lectin from the hemolymph of crustaceans was most successful by affinity chromatography as it gave a higher fold of purificat...

Ngày tải lên : 21/02/2014, 00:20

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Tài liệu Báo cáo Y học: Identiﬁcation and characterization of a mammalian 14-kDa phosphohistidine phosphatase pdf

... GRRPsLFG and APDyTTPEMY-NH 2 were obtained from Dr A ˚ ke Engstro ¨ m at the Peptide Synthesis and Analysis Laboratory of the Department of Medical Biochemistry and Microbio- logy, Uppsala University. The ... obtained from Promega, and LysC of Achromobacter lytii from Wako. The O-phosphorylated phosphopeptides EQTRsLDGR-NH 2 , DHTGFLtEY- VATRWC-NH 2 , DADEyL-NH 2 , EGDNDyI...

Ngày tải lên : 21/02/2014, 01:21

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Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a membrane-bound enzyme complex from the sulfate-reducing archaeon Archaeoglobus fulgidus related to heterodisulﬁde reductase from methanogenic archaea pdf

... to identify the physiological electron acceptor of the enzyme. The enzyme was not oxidized by the heterodisulfide of coenzyme M and coenzyme B, or by the homodisulfides of these two coenzymes. Also ... catalyzed by methyl-coenz yme M reductase [1]. This disulfide is reduced by a unique disulfide reductase, designated heterodisulfide reductase (Hdr). Two types of Hdr from phylo genetica...

Ngày tải lên : 21/02/2014, 03:20

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Tài liệu Báo cáo Y học: Identiﬁcation and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx

... com- plexes may represent true physiologically relevant interactions in stationarily grown cells. Identiﬁcation of proteins X (GatY) and Y (UP12) In order to identify polypeptides X and Y, we repeated ... of ybdQ deletion on cell growth at 37 °C and on sensitivity towards CCCP exposure. (A) Growth curves of wild-type and D ybdQ cells in Luria–Bertani broth. Single colonies o...

Ngày tải lên : 22/02/2014, 07:20

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Báo cáo khoa học: Puriﬁcation and characterization of zebraﬁsh hatching enzyme – an evolutionary aspect of the mechanism of egg envelope digestion pot

... molecular phylogenetic analysis of ﬁsh hatching enzyme genes, hatching enzyme originally consisted of a single type of enzyme, and HCE and LCE were produced by duplication and diversiﬁcation of the ... (Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, Tokyo) for reading the manuscript and to Dr K. Yamagami (former Profess...

Ngày tải lên : 07/03/2014, 04:20

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Báo cáo Y học: Puriﬁcation and characterization of novel kininogens from spotted wolfﬁsh and Atlantic cod pdf

Tài liệu Báo cáo Y học: Puriﬁcation, characterization, cloning, and expression of the chicken liver ecto-ATP-diphosphohydrolase pot

Tài liệu Báo cáo Y học: Puriﬁcation, characterization, immunolocalization and structural analysis of the abundant cytoplasmic b-amylase from Calystegia sepium (hedge bindweed) rhizomes ppt