Báo cáo Y học: Amphipathic property of free thiol group contributes to an increase in the catalytic efficiency of carboxypeptidase Y pot

... doi:10.1046/j.1432-1033.2002.02997.x designed to maintain hydrophobicity and Gly, Ser, Asp and His mutants to provide hydrophilicity. The catalytic roles of Cys341 in carboxypeptidase Y are examined by comparing the kinetic parameters of ... Amphipathic property of free thiol group contributes to an increase in the catalytic efficiency of carb...

Ngày tải lên: 08/03/2014, 23:20

... peripheral nervous system injury, and reducing the biosynthesis of ICAM-1 and other cytokines by blocking the cell signal pathway might provide a new strategy against in ammatory and immune reaction ... p38 and JNK leads to an increase in ICAM-1 expression in Schwann cells of sciatic nerves Aiguo Shen 1, *, Junling Yang 2, *, Yangyang Gu 3 , Dan Zhou 4 , Linlin Sun 2 , Yong...

Ngày tải lên: 18/02/2014, 18:20

... domain, converting some of the enzyme to the active form. The  3-fold increase in activity may arise from the binding of the FMN domain to hMS facilitating binding of AdoMet and ⁄ or methyl transfer, ... cobalamin affinity chromatography, as judged by SDS ⁄ PAGE analysis (Fig. 2, inset). The absorption spectrum of the purified enzyme was typical of the hydroxy...

Ngày tải lên: 18/02/2014, 08:20

... Consequently, strategies aiming to over- come tumour cell resistance to chemotherapy and to increase drug efficacy, thereby minimizing toxic effects, are critically important. The molecular mechanisms of 5-FU ... been used in the clinic for several decades, and its metabolic pathways and cyto- toxic modes of action through the inhibition of thymi- dylate synthase activity...

Ngày tải lên: 07/03/2014, 00:20

... as an input, starts from the initial state with its head scanning the root node of an input. Ac- cording to the current state and the label of the scanned node, it transforms an input tree into ... locally satisfies the functional schemata of all productions in sp. M transforms r into the yield of t, i.e., the terminal string ob- talned by concatenat...

Ngày tải lên: 08/03/2014, 07:20

... of the solubilization of SugE, Y K and TehA in micelles and of their reconstitution into membranes. The dissolving of the proteins in suitable detergents obviously resulted in the refolding of ... part of the synthesized protein ‘remained soluble and this amount could be significantly increased by the addition of E. coli lipids into the cell -free reaction....

Ngày tải lên: 30/03/2014, 13:20

... for the catalytic action of plas- min in the presence of stearate. The p-nitroaniline (P) released in the course of hydrolysis of 40 l M Spectrozyme-PL by 20 nM plas- min in the presence of 115 ... manner, the instability of the enzyme in the assay system was also consid- ered according to the scheme suggested by Duggleby [13,14] in which E ¢ indica...

Ngày tải lên: 18/02/2014, 17:20

... between the volume change introduced by the mutation in position 266 and the decrease in enzyme catalytic efficiency. Remarkably, the value for the catalytic efficiency pre- viously obtained by replacement ... primarily involved in nicotinamide binding rather than being directly involved in the hydride transfer reaction [21]. Our results, together with the amino ac...

Ngày tải lên: 18/02/2014, 17:20

... quantification of the local background and evaluation of the array homoge- neity, which is taken into account in the normalization. Furthermore, in order to consider the purity and quality of the ... using a factor calculated from the intensity ratios of internal standards in the test and refer- ence samples. The presence of the internal standard probes at d...

Ngày tải lên: 07/03/2014, 05:20

... are much lower than for prokaryotic targets. In the inter- est of efficiency and cost savings, it is important to analyze where failures occur and to devise strategies to minimize these. The most effective ... exhibiting £ 30% sequence identity to the sequence of any protein with a three-dimensional structure deposited in the Protein Data Bank. Solubil- ity can be imp...

Ngày tải lên: 07/03/2014, 12:20