Báo cáo Y học: Expression of the V-ATPase proteolipid subunit of Acetabularia acetabulum in a VMA3-deficient strain of Saccharomyces cerevisiae and study of its complementation pdf
Báo cáo y học: "Intrathecal siRNA against Toll-like receptor 4 reduces nociception in a rat model of neuropathic pain"
... administered intrathecally once daily for 7 days, starting from 1 day before CCI surgery. Evaluation of tactile allodynia and thermal hyperalgesia The paw withdrawal latency (PWL) to radiant heat and ... 5’-CUACCAACAGAGAGGAUAU-3” (siRNA1), 5’-GUCUCAGAUAUCUAGAUCU-3’ (siRNA2), 5’-GAGCCGGAAAGUUAUUGUG-3’ (siRNA3). All siRNAs were chemically synthesized by United Gene Company (Sha...
Ngày tải lên: 25/10/2012, 11:48
Báo cáo Y học: Substrates modulate the rate-determining step for CO binding in cytochrome P450cam (CYP101) A high-pressure stopped-flow study pdf
... structural parameters of the protein and the substrate which are responsible for the uncoupling process are not well understood. Data are increasingly accumulated indica- ting that the dynamics of the ... negative activation volumes and slow rebinding kinetics while the substrates norcamphor and adamantane and the substrate-free protein have positive activation volum...
Ngày tải lên: 08/03/2014, 23:20
Báo cáo y học: "Expression of hMSH2 protein of the human DNA mismatch repair system in oral lichen planus"
... primary incubation in the presence of non-immunized rabbit serum instead of the primary antibody. Cell quantification and Statistical analysis The immunolocalization of hMSH2 was quantitatively ... membrane, degeneration of basal keratinocytes, hyperkeratinization and acanthosis [24]. Basal cells are the prime target of destruction in OLP. The mechanism of bas...
Ngày tải lên: 03/11/2012, 09:54
Tài liệu Báo cáo Y học: Expression and characterization of active site mutants of hevamine, a chitinase from the rubber tree Hevea brasiliensis docx
... 5¢-GGTTGAACCATGCTCTATGTTAAAATCAATACCATCCAA-3¢ Glu127Ala Sense strand 5¢-GGTATTGATTTTGACATAGCGCTATGTCAAAATCAATACC-3¢ Anti-sense strand 5¢-GTACAGGGTTGAACCATGCGCTATGTCAAAATCAATACC-3¢ Asp125Ala/Glu127Ala Sense strand ... 5¢-GATGGTATTGATTTTGCCATAGAGCATGGTTCA-3¢ Anti-sense strand 5¢-TGAACCATGCTCTATGGCAAAATCAATACCATC-3¢ Asp125Asn Sense strand 5¢-TTGGATGGTATTGATTTTAACATAGAGCATGGTTCAACC-3¢ Anti-sense...
Ngày tải lên: 22/02/2014, 04:20
Báo cáo Y học: Expression of the V-ATPase proteolipid subunit of Acetabularia acetabulum in a VMA3-deficient strain of Saccharomyces cerevisiae and study of its complementation pdf
... Expression of the V-ATPase proteolipid subunit of Acetabularia acetabulum in a VMA3 -deficient strain of Saccharomyces cerevisiae and study of its complementation Mikiko Ikeda 1 , Misato Hinohara 1 , ... was examined using a Saccharomyces cerevisiae VMA3-deficient strain that lacked its own gene for one of the proteolipid subunits of...
Ngày tải lên: 08/03/2014, 23:20
Báo cáo Y học: Expression and purification of the recombinant subunits of toluene/ o-xylene monooxygenase and reconstitution of the active complex potx
... using a SMART-System (Pharmacia Biotech). The molecular mass markers used as standards for gel filtration chromatography were b-amylase (200 kDa), aspartate aminotransferase (90 kDa), ribosome inactivating protein ... by the program GRAPHPAD PRISM (http://www.graphpad.com). Single turnover assay Single turnover assays of the individual components (10nmolofTomoH,20nmolofTomoCandTomoD...
Ngày tải lên: 17/03/2014, 10:20
Báo cáo Y học: Expression pattern in the antennae of a newly isolated lepidopteran Gq protein a subunit cDNA potx
... cDNA revealed that there is a polyadenylation signal upstream of the poly (A) . Analysis of the primary structure of M. brassicae Gqa The putative protein product encoded by the cloned cDNA was aligned ... expres- sion localization in the antennae of the first lepidopteran G protein a subunit belonging to the Gq family. MATERIALS AND METHODS Insects Animals were...
Ngày tải lên: 17/03/2014, 23:20
Báo cáo Y học: Expression of the aspartate/glutamate mitochondrial carriers aralar1 and citrin during development and in adult rat tissues docx
... We investigated the pattern of expression of aralar1 and citrin in murine embryonic and adult tissues at the mRNA and protein levels. Insituhybridization analysis indicates that both isoforms are ... from the embryos in (A and a) at the axial levels indicated by the dotted lines. Note the strong expression in the limb (B) and tail buds (arrowhead in...
Ngày tải lên: 24/03/2014, 04:21
Báo cáo Y học: Expression of the uncoupling protein 1 from the aP2 gene promoter stimulates mitochondrial biogenesis in unilocular adipocytes in vivo potx
... microscopy the surface area of about 130±170 cells for each animal was measured by an Image Analyzer KS100 IBAS Kontron ( Karl Zeiss Jena, Germany), in o rder to calculate the diameter of the adipocytes. ... 3¢,3¢-diaminobenzidine hydrochloride c hromogen (Sigma). The speci®city of the method was tested by the omission of the primary antibody in the staining, and...
Ngày tải lên: 31/03/2014, 15:20
Báo cáo y học: "Expression of Human Globular Adiponectin-Glucagon-Like Peptide-1 Analog Fusion Protein and Its Assay of Glucose-Lowering Effect In Vivo"
... Statistical analysis Data were expressed as means ± standard devi- ations. Data were analyzed using one-way analysis of variance and secondary analysis for significance with the Turkey-Kramer ... [1]. Adiponectin is a protein of 247 amino acids consisting of four domains, an ami- no-terminal signal sequence (1-18 amino acid), a var- iable region (19-41 amino acid), a colla...
Ngày tải lên: 25/10/2012, 11:10