Báo cáo Y học: Structural and biochemical characterization of calhepatin, an S100-like calcium-binding protein from the liver of lungfish (Lepidosiren paradoxa) docx

... high sequence identity, Fig. 1, and many other residues display conservative changes. The region between residues 54 and 79 of CR I – II, encompassing the linker, helix C and start of the second calcium-binding ... allowed us to analyze the biochemical and structural properties of CR I–II and to compare them with Calb I–II. Our data show that CR I–II and Calb I...

Ngày tải lên: 22/02/2014, 07:20

... Structural and biochemical characterization of calhepatin, an S100-like calcium-binding protein from the liver of lungfish ( Lepidosiren paradoxa ) Santiago M. Di Pietro and Jose ´ A. Santome ´ Instituto ... a calcium-binding protein of the S100 family, isolated from lungfish (Lepidosiren paradoxa) liver. The primary struc- ture, determined b...

Ngày tải lên: 08/03/2014, 22:20

... is the measured anisotropy value, A F is the aniso- tropy of the free minifiber, A B is the anisotropy of the mini- fiber bound to the penton base, [Lig B ] is the concentration of the bound ligand ... (minifiber), and [Lig tot ] is the total con- centration of the ligand. At any total concentration of lig- and, the anisotropy depends on the total concentrati...

Ngày tải lên: 19/02/2014, 06:20

... functionally similar in that they bind ice and depress the freezing point. They are found in a variety of insect and plant species as well as bacteria and fungi, but fish AFPs were the first and most ... ruthenium red dye. Analysis of antifreeze activity Antifreeze activity was quantitated a s thermal hysteresis, which is the difference b etween the melting and freezing po...

Ngày tải lên: 24/03/2014, 03:21

... resides in erythrocytes in mammals, possess only a limited set of enzymes involved in amino acid synthesis of Ser from Gly and Ala from Cys and conversions between Asp and Asn and between Glu and Gln ... for the latter relationship in the NJ analysis (88%, Fig. 3). The ML tree demonstrates that the common ancestor of Type IIB and Type III is located within Type I...

Ngày tải lên: 19/02/2014, 13:20

... Kinetic and biochemical analyses on the reaction mechanism of a bacterial ATP-citrate lyase Tadayoshi Kanao, Toshiaki Fukui, Haruyuki Atomi and Tadayuki Imanaka Department of Synthetic Chemistry and ... cytosol as starting materials for a variety of biosynthetic pathways. Rat and human ACLs from various organs and tissues have been extensively studied in terms of bioc...

Ngày tải lên: 08/03/2014, 22:20

... by a cycle of polymerization and depolymerization, isoform separation by hydroxyapatite chromatography, and a final gel filtration step. Actin cosedimentation and cross-linking assays Samples of ... EF-hand proteins. The second pair of EF-hands in calmodulin (PDB code 1CLL) [19] and the second pair of EF-hands in troponin C (PDB code 1NCX) [20] are structurally closely relat...

Ngày tải lên: 16/03/2014, 06:20

... Nikolay P. Arbatsky 2 , Alexander S. Shashkov 2 and Yuriy A. Knirel 2 1 Department of General Microbiology, Institute of Microbiology and Immunology, University of èodz  , Poland; 2 N. D. Zelinsky Institute ... LPS of P. penneri 40 and 41 abolished binding of these antigens and signi®cantly decreased binding of the antigens of P. penneri 1and4 . In Western b lot analy...

Ngày tải lên: 17/03/2014, 17:20

... proportion of 54 and 56 acyl carbon triacylglycerols, Table 2. Fatty acid composition of VLDL and liver triacylglycerols. Fatty acid methyl esters were prepared from VLDL and liver triacylglycerols by acidic ... recovered from a HPLC separation as described by Yang and Kuksis [26]. The molecular associ- ation of the fatty acids and reverse isomer content of the...

Ngày tải lên: 23/03/2014, 21:20

... enzymes of P. denitrificans exhibit an activity of Fe(III) reductase. These enzymes were purified and their molecular and catalytic properties were analyzed. The data obtained indicate that one of ... uniquely attributable to this enzyme. On the other hand, the solution of FerB was yellowish in color and exhibited absorption bands at 380 and 448 nm (Fig. 3), diagnostic f...

Ngày tải lên: 30/03/2014, 13:20