Báo cáo Y học: A novel DNA repair enzyme containing RNA recognition, G-patch and specific splicing factor 45-like motifs in the protozoan parasite Toxoplasma gondii potx
Báo cáo Y học: A novel DNA repair enzyme containing RNA recognition, G-patch and specific splicing factor 45-like motifs in the protozoan parasite Toxoplasma gondii potx
... A novel DNA repair enzyme containing RNA recognition, G-patch and specific splicing factor 45-like motifs in the protozoan parasite Toxoplasma gondii Najoua Dendouga1, Isabelle Callebaut2 and ... DNA repair enzyme) for consis-tency with the Arabidopsis thaliana enzyme, belongs to a large family of proteins containing RNA recognition motifs (RRM), glycine-rich motifs (G-patch) and a specific ... protozoan parasites such asT. gondii and the Plasmodia. Further analyses shouldcontribute to our understanding of the biochemistry of DNA repair proteins in medically relevant parasites, and thus...
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Báo cáo y học: " A Novel Variable Number of Tandem Repeat of the Natriuretic Peptide Precursor B gene’s 5’-Flanking Region is Associated with Essential Hypertension among Japanese Females"
... mainly in the brain [4,5]. BNP, which was originally isolated from the por-cine brain [6], shows an amino acid sequence homol-ogy to ANP. BNP has central and peripheral actions that are ... Watanabe and Dr. Y. Izumi for collecting the samples, and Ms. H. Tobe, M. Nakamura, and K. Sugama for their technical as-sistance. This work was supported financially by a grant from the Ministry ... presented as the mean ± SD. The Hardy-Weinberg equilibrium was assessed by doing chi-square (χ2) analysis. Differences in the clinical data between the EH and NT groups were assessed by analysis...
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Tài liệu Báo cáo Y học: A novel meta-cleavage dioxygenase that cleaves a carboxyl-groupsubstituted 2-aminophenol Purification and characterization of 4-amino-3-hydroxybenzoate 2,3-dioxygenase from Bordetella sp. strain 10d doc
... sequences of other proteinsincluding those of extradiol dioxygenases available in the FASTA AND BLASTdatabase programs at the DNA DataBank of Japan. The gene encoding 4-amino-3-hydrox-ybenzoate 2,3-dioxygenase ... homogeneity with a 110-fold increase in specific activity. The purified enzyme was characterized as a meta-cleavage dioxygenase that catalyzed the ring fissionbetween C2 and C3 of 4-amino-3-hydroxybenzoic ... both native and denaturing polyacrylamide gels (Fig. 2). The apparentmolecular mass was determined to be 40 kDa by gel filtration and 21 kDa by SDS/PAGE. These findings indicated that the enzyme...
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Tài liệu Báo cáo Y học: A novel, inducible, citral lyase purified from spores of Penicillium digitatum docx
... [18].For the enzymatic equivalent of this reaction the actionsof a hydratase and an aldolase are needed. Citral lyaseof P. digitatum combines hydratase and aldolase activity in a single enzyme. ... induction.Stability of citral lyase activity The activity and stability of citral lyase was dramaticallyaffected by the addition of 20% (v/v) glycerol and 1 mMEDTA. When glycerol and EDTA were added ... beenreports on enzymes combining the action of a hydratasewith that of an aldolase. The best studied is enoyl-CoAhydratase/aldolase [26] (also known as 4-hydrox-ycinnamoyl-CoA hydratase/aldolase [27]),...
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Báo cáo Y học: A novel gain-of-function mutation of the integrin a2 VWFA domain docx
... to the VWFA domain by a similar mechanism t o collagen I. Thesedata indicate that residue E318 plays a novel and importantrole in mod ulating a2 VWFA domain–ligand b inding and may b e involved ... W,5¢-TTCAATGTGTCTGATTGGGCAGCTCTACTAGAAAAGGCTG-3¢; for E318 to I, 5¢-CAATGTGTCTGATATAGCAGCTCTACTAGAAAAG-3¢; for E318 to R,5¢-CAATGTGTCTGATCGAGCAGCTCTACTAGAAAAG-3¢; for E318 to Y, 5¢-CAATGTGTCTGATTATGCAGCTCTACTAGAAAAG-3¢. ... myocardial infarction : a case con trol study. Lancet 353,351–353.20. Matsubara, Y. , Mur ata, M., Maruyama, T., Handa, M.,Yamagata, N ., Watanabe, G ., Saruta, T. & Ikeda, Y. (2000)Association...
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Báo cáo khoa học: A novel metallocarboxypeptidase-like enzyme from the marine annelid Sabellastarte magnifica – a step into the invertebrate world of proteases pdf
... gamus, Molgula occidentalis and Pyura vittata); 11 species of Cnidaria (Bartholo-mea annulata, Budonosoma granulifera, Cassiopeaxamachana, Condylactys gigantea, Gorgonia ventalina,Lebrunia ... by the cystatin one; and 8, 9, 10, 11, 12 and 13 by the SBTI one). An initial validation of these assign-ments as proteolytic enzymes (awating MS ⁄ MS analy-sis) was made by ‘intensity fading’ ... 2009)doi:10.1111/j.1742-4658.2009.07187.xAfter screening 25 marine invertebrates, a novel metallocarboxypeptidase(SmCP) has been identified by activity and MS analytical approaches, and isolated from the marine annelid Sabellastarte...
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Báo cáo Y học: A b-lysine adenylating enzyme and a b-lysine binding protein involved in poly b-lysine chain assembly in nourseothricin synthesis in Streptomyces noursei pot
... domainsincluding the adenylation domain (A- domain) responsiblefor amino-acid recognition and their activation as anaminoacyl adenylate, and the peptidyl carrier domain(PCP-domain or T-domain) ... of b-lysine. The b-lysine binding protein was assayed in a coupledassay with the b-lysine activating enzyme. The assaycontained 1±2 pkatal of b-lysine activating enzyme, 0.1 mMb-ly sine, 0.1 ... containing a carbamido-D-gulos-amine core, to which a poly b-lysine chain and the unusualamino acid streptolydine are attached in amide and N-glycosidic linkages, respectively (Fig. 1). The...
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Báo cáo khoa học: A novel pathway for sequential transformation of 7-dehydrocholesterol and expression of the P450scc system in mammalian skin pptx
... controlplacenta, whole human skin, normal epidermal and immor-talized keratinocytes, dermal fibroblasts, squamous cellcarcinoma and five human melanomas. Thus, these dataclarify in detail the cutaneous ... [29]. The chem icalstructure of the standard had been c onfirmed by N MRanalysis. The standard was f urther purified by RP-HPLCandstoredat)70 °C.Enzymatic assaysSide-chain cleavage of 7-DHC. Large-scale ... dreno-doxin reductase and adrenodoxin [8,9]. This biochemicalpathway may be operative in the skin, as it expresses the related CYP1 1A1 , CYP17, CYP2 1A2 and MC2-R genes[10]. Furthermore, skin and skin...
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Báo cáo Y học: Characterization of four substrates emphasizes kinetic similarity between insect and human C-domain angiotensin-converting enzyme pptx
... evolutionary distance, arestructurally remarkably similar. The molecular biologicalanalyses of insect ACEs revealed a high cDNA and aminoacid sequence conservation with mammalian ACE, especi-ally ... human sACE, cACE and nACE and with the purified Locusta migratoria testis ACE using a compe-tition based assay. The cleavage of a fluorogenic ACE-substrate was followed in the absence and in the ... previously [15].Purification of mammalian and insect ACE enzymesChinese hamster ovary cells expressing recombinant humansACE, cACE and nACE were a kind gift of P. Corvol and A. Michaud (Institut National...
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Báo cáo y học: "A Comparative Effectiveness Study of Bone Density Changes in Women Over 40 Following Three Bone Health Plans Containing Variations of the Same Novel Plant-sourced Calcium"
... supple-ments in these plans were analyzed by Exova Labs, Chicago, IL for confirmation of nutrient levels and absence of heavy minerals and other contaminating ingredients. Calcium, magnesium and other ... (commercially known as „AlgaeCal‟ or „AC‟) made by milling whole, live-harvested sea algae found on the South American coastline. In addition to calcium, the algae contained other naturally occurring ... measure was changes in baseline BMD (Bone Mineral Density) among participants with above average compliance in order to examine the safety and efficacy in people complying with the bone health...
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