Báo cáo Y học: Properties of group I allergens from grass pollen and their relation to cathepsin B, a member of the C1 family of cysteine proteinases pdf

... resemble ancient and modern cathepsin B, which is a member of the papain (C1) family of cysteine proteinases. Using the Pichia pastoris expression system, we show that cleavage of inhibitory prosequences ... pronase. Protozoan parasites of the genus Giardia are one of the earliest lineages of eukaryotic cells, and the Giardia protease is the earliest k...

Ngày tải lên: 08/03/2014, 22:20

... USA) and calpain I inhibitor was from Calbiochem (San Diego, CA, USA). Anti Na + /K + ATPase Ig (6F) was from the Develop- mental Studies Hybridoma Bank (Iowa City, Iowa, USA). Rabbit polyclonal ... protein has an N-terminal, hydrophobic, membrane-associated domain of about 500 amino acids, and a C-terminal, hydrophilic domain that contains about 315 amino acids. The membran...

Ngày tải lên: 08/03/2014, 16:20

... supercoils are maintained actively at the cost of ATP hydrolysis, via topoisomerase activities. Four topoisomerases h ave been identi®ed in Escherichia coli (reviewed in [2]). Topoisomerase I [3,4] and ... mgámL )1 rifampicin. Topoisomerase concentration The t opoisomerase I and DNA gyrase content of the cells was estimated by quantitative W estern blotting using a n antib...

Ngày tải lên: 18/03/2014, 01:20

... in humans: role of hyperglycemia and hyperinsulinemia. Circulation 2000;101:2247-51. 8. Takahashi K, Mizuarai S, Araki H, Mashiko S, Ishihara A, Kanatani A, Itadani H, Kotani H. Adiposity ... atherosclerotic plaques, mainly by macrophages and foam cells [3]. The MMP family is catalytically active, both in vitro and in vivo. The activity of MMPs is increased by gene tran...

Ngày tải lên: 26/10/2012, 10:03

... synaptosomes Brevetoxin-1 (PbTx-1) from a marine dinoflagellate, inhibits allosterically the b inding of the s corpion a- tox in, Aah-II, to rat brain synaptosomes [27,28]. To examine the similarity in binding to ... inhibitory constant; k off , dissociation rate constant; k on , association rate constant; Lqh-II, Lqh-III, LqhaIT, a classical a- toxin, an a- like toxin,...

Ngày tải lên: 08/03/2014, 16:20

... (5¢-CTTGACAGATCTTAGGAGATCA GCAAACTAC-3¢) was used together with the T7 primer to amplify the cDNA. After purification from the gel, the PCR fragment was digested with BglII and SalI and ligated to a ... which are again very similar to each other (91% identity). In a phylogenetic analysis the separate clustering within the tpsb family of C62 and M34 from B93...

Ngày tải lên: 08/03/2014, 23:20

... by the results of tests, including x-ray, angiography, ultrasound examination, the electrocardiogram, and occasionally a biopsy. The availability of the laboratory examination probably differs ... listed, nor was ethnicity listed. All of the data were submitted to the Panamanian Ministry of Health in Panama City and were available for analysis. The code of...

Ngày tải lên: 31/10/2012, 15:12

... such as transactivation domains, are very variable in their amino- acid sequence and their location could not be predictable from amino-acid sequence comparisons. As an approach to the comparative ... C/EBPa to activate transcription. In summary, the data obtained in this study indicate the conservation of the SRF DNA-binding and dimerization domains during evolution...

Ngày tải lên: 22/02/2014, 07:20

... addition. Synchronization was confirmed by FACS analysis. RNA isolation Total RNA was isolated from primary MEFs with the RNeasy kit (Qiagen) according to the manufacturer’s instructions. To isolate ... additional amino acids. The E2F6 5¢ untranslated region impairs ribosomal scanning Translation of eukaryotic mRNAs is usually initiated at the first AUG triplet downstream of...

Ngày tải lên: 08/03/2014, 09:20

... Universite ´ di Pisa, Italy The X-ray structure of variant A of authentic boar salivary lipocalin (SAL), a pheromone-binding protein specifically expressed in the submaxillary glands of the boar, ... automated docking performed with CNS started from this position and involved positional minimization of the ligand and the protein. During the initial minimization st...

Ngày tải lên: 17/03/2014, 17:20