Báo cáo Y học: Purification and biochemical characterization of some of the properties of recombinant human kynureninase pptx

... Purification and biochemical characterization of some of the properties of recombinant human kynureninase Harold A. Walsh and Nigel P. Botting School of Chemistry, University of St Andrews, ... regulation of enzyme activity in vivo and consequent channeling of substrate 3-hydroxykynurenine down the tryptophan– kynurenine metabolic pathway. The purifica...

Ngày tải lên: 08/03/2014, 22:20

... (saponification) of the N-glycans yielded glycans devoid of additional acetyl groups, an indication that these were O-acetyl substituents [15]. The MALDI-TOF spectrum of the de-O-acetylated glycans reveals the ... belonging to class 3 of the cystatin superfamily. They are single-chain proteins composed of an N-terminal heavy chain, the bradykinin moiety and a C-terminal...

Ngày tải lên: 08/03/2014, 23:20

... galactose. Protein and carbohydrate analysis The concentration of protein was measured by the method of Lowry et al. [13] using BSA as the standard. The presence of sugar in the protein was detected by periodic ... In the other side of the tank, a light source was placed in order to attract the nauplii. Two days were allowed for the hatching of all the eggs an...

Ngày tải lên: 18/03/2014, 01:20

... VanX- and VanY-type enzymes indicated that all of these enzymes contained the same Zn 2+ binding motif. VanX homologues present in the cyanobac- terium Synechocystis strain PCC6803, and the glycopeptide antibiotic ... a D -Ala- D -Ser standard curve. For determination of the rate of hydrolysis of UDP-MurNAc- pentapeptide[Ala], His 6 –VanXY C was used at a concentra- tion...

Ngày tải lên: 18/03/2014, 01:20

... versatile enzymes in nonaqueous solvent systems in which they catalyze the synthesis of a variety of acylglycerols and specialized esters via transesterification. Lipases demonstrating high activity under ... in detergents, one of the largest industrial uses of these enzymes [4]. Lipase-catalyzed synthesis of structured triacylglycerols comprised of both long and medium cha...

Ngày tải lên: 23/03/2014, 21:20

... of the co o genes encoding the subunits of the CO-oxidizing:H 2 -evolving enzyme complex of C. hydrogenoformans. The cooMKLXUHhypA and the cooFISI gene cluster are located on contig 2340 of the ... enzyme complex from C. hydrogenoformans. Theenzymewaspurifiedfrom40gcells(wet mass). One unit of enzyme activity is defined as the amount of enzyme that catalyzes the re...

Ngày tải lên: 23/03/2014, 21:20

... below. AP assays All assays were linear with respect to time and concentration of enzyme assayed. One unit (U) of activity is defined as the amount of AP resulting in the hydrolysis of 1 lmol of substrate ... °C. Phosphatase assay A For routine measurements of AP activity, the hydrolysis of phosphoenolpyruvate to pyruvate was coupled to the lactate dehydrogenase reactio...

Ngày tải lên: 23/03/2014, 21:20

... determination of hA2aR by X-ray or electron crystallography, or infor- mation on the bound ligand conformation obtained by NMR spectroscopy, would assist in the design of subtype speci®c compounds and ... cally to the XAC-agarose. Theophylline was removed by gel ®ltration before quanti- fying speci®c [ 3 H]ZM241385 binding in the X AC-agarose eluate. The value obtained was onl...

Ngày tải lên: 24/03/2014, 00:21

... speci®city of the enzyme When the hydrolytic activities of the proteinase were measured at pH 9.0 with various synthetic substrates, the results listed in Table 5 were obtained. The enzyme hydrolyzed ... containers. Hence, some of the s tability features of the enzyme may be affected by small amounts of contaminating proteins. Fig. 3. The eect of pH on the pro...

Ngày tải lên: 31/03/2014, 21:21

... oligomannose/hybrid glycostructure, whereas the serum enzyme and the liver enzyme belong to the groups of glycoproteins with a glycostructure of the complex type. To substantiate the notion that the TRH-DEs ... from rat and pig brain [28]. For the purification of the serum TRH- DE fractionation by poly(ethylene glycol) precipitation was used as the first step not o...

Ngày tải lên: 31/03/2014, 21:21