Báo cáo Y học: Electrostatic properties of the structure of the docking and dimerization domain of protein kinase A IIa doc

... C-terminal site of the dimerization and docking domain. This could allow the interdomain/cAMP binding region to pivot about the stable AKAP-bound dimerization and docking domain, and to weakly associate with ... discovery of dual-specificity AKAPs (D-AKAPs) indicates that the type I enzyme may also be localized [4]. The N-terminal dimerization domain of the...

Ngày tải lên: 08/03/2014, 22:20

... C62 and M34 was 5¢-GCCAGGATCCAATGAGGAGATCAGCAAACTA CC-3¢, containing a BamHI restriction site. The sense primer for D85 contained a BamHI restriction site 5¢-GCCAGGATCCAATGAGGCGATCTGCTGATTA CG-3¢. ... GC-MS as described above and on a radio-GC consisting of a Carlo-Erba 4160 Series gas chromatograph (Carlo-Erba, Milano, Italy) equipped with a RAGA-90 radioactivity detector (...

Ngày tải lên: 08/03/2014, 23:20

... problem. 39 After craniotomy, removal of the in- tracranial lesion, and duraplasty, the bone flap was replaced and one side of the flap was attached to the cranium by plates. The plates act as a hinge ... fascia from the temporal muscle to the zygomatic arch, and then cut the fascia from the base backwards along th e zygoma but left the fascia base 1-2 cm...

Ngày tải lên: 25/10/2012, 11:40

... of the water-soluble matrix, the EDTA-soluble matrix and the EDTA-insoluble matrix of Pinctada maxima nacre. Sulfated and nonsulfated glycosaminoglycans from the supernatant were estimated by ... glutamic acid- rich in WSM. Glycosaminoglycan analysis Glycosaminoglycans are highly negatively charged because of the presence of sulfate ester and/ or carboxyl groups. Theref...

Ngày tải lên: 21/02/2014, 01:21

... [1] in granular corneal dystrophy type 2 (GCD2, Avellino dystrophy) a mixed type of amyloid and granular deposits, and Arg124Leu (R124L) [13] in corneal dystrophy of Bowman’s layer type 1 (CDB1, ... presented an intermediate degree of amyloid fibril formation. Cysteine has the capacity to form disulfide bonds. As disulfide bonding of the protein may participate in the appea...

Ngày tải lên: 21/02/2014, 01:21

... Avda, Salamanca, Spain; 2 Centro Hispano-Luso de Investigaciones Agrarias, Universidad de Salamanca, Edi®ci o Departamental, Avda, Salamanca, Spain The Phycomyces blakesleeanus wild-type is yellow, ... A domains of the genes carRP or carRA of Mucor and Phycomyces, respectively, are white, accumulate no caro- tenoid or only traces of b-carotene, and are altered in t he enzyme p...

Ngày tải lên: 22/02/2014, 04:20

... to the m anufacturer’s i nstructions. The primers 5¢-TTAGGATCCAGAAAAATGGGG-3¢ and 5¢-AACAAACAGCGGCCGCGGGCACTTTAATCC-3¢ were used in PCR to amplify the coding region of the cDNA and introduce appropriate ... mixture was determined by measuring its ability to activate PFP. For each determination of 6PF2K and Fru-2,6-P 2 ase activity, the activation of PFP was calibrated ag...

Ngày tải lên: 22/02/2014, 04:20

... GTA TGG AAG TGT GGA ATA GTT T 3¢ C9L 5¢ GGA CAC CAC AGA TAT CCA ATA G 3¢ C1011U 5¢ CAT AAA ACT AAC AAT ACA TGT 3¢ C1011L 5¢ TAG CAG ATA TTG AAA AGG AG 3¢ C12U 5¢ CCA GTG AAT ATT CAG GAC TGA T ... AGG AAC TGA ACA GAA CAT TG 3¢ C1L 5¢ CAA CTG CGT ATT AGT GAA GAA TG 3¢ C2U 5¢ AAT CAA ATT TTA TTA TAA GAC C 3¢ C2L 5¢ AAT AGC TAA TTA GAG ACC AT 3¢ C3U 5¢ GGT GTC AAA TTT AGC TGT TAA A 3¢ C3L ... C...

Ngày tải lên: 22/02/2014, 04:20

... medulla, and papilla in almost the same quantity. HSP60 was remarkably decreased in the kidney papilla of rats that were water restricted but the protein was increased in the mitochondria of the rat ... H., Kobayashi, R., Wakui, H., Komatsuda, A. , Ohtani, H., Miura, A. B., Otaka, M., Masamune, O., Andoh, H., Koyama, K., Sato, Y. & Tashima. Y. (1995) Mammalian 60-kDa...

Ngày tải lên: 08/03/2014, 09:20

... activity of PB2-T1 and PB2-T2 toward various peptide substrates designed according to the a- , b-, and c-secretase cleavage site of APP was investigated. The substrate cleavage was assayed and quantified ... Lin 1,2,4 1 Functional Proteomics Laboratory and Crystallography Program, Oklahoma Medical Research Foundation, Oklahoma City, USA; 2 ProteomTech, Inc., Oklahoma City, USA...

Ngày tải lên: 17/03/2014, 10:20