Báo cáo Y học: Crystal structure of the catalytic domain of a human thioredoxin-like protein pdf
Báo cáo Y học: Crystal structure of the catalytic domain of a human thioredoxin-like protein pdf
... Crystal structure of the catalytic domain of a human thioredoxin-like protein Implications for substrate specificity and a novel regulation mechanism Jian Jin 1,2 , Xuehui Chen 1 , Yan Zhou 2 , ... domain remains unknown. Overall structure Crystals of the catalytic domain of hTRXL (hTRXL-N) were obtained from ammonium sulfate by hanging-drop vapor-diffusio...
Ngày tải lên: 08/03/2014, 22:20
Báo cáo khoa học: Crystal structure determination and inhibition studies of a novel xylanase and a-amylase inhibitor protein (XAIP) from Scadoxus multiflorus pot
... a- amy- lase. These observations indicate that XAIP associates with GH11 xylanase and GH13 a- amylase, as well as with both xylanase and a- amylase simultaneously. Tissue distribution of XAIP The output of ... of a- amylase in a 1 : 1.2 molar ratio. The inhibition of a- amylase by XAIP was also observed in the presence of GH11 xylanase. Thus, the inhibition of a- a...
Ngày tải lên: 29/03/2014, 09:20
Tài liệu Báo cáo khoa học: Dimer asymmetry and the catalytic cycle of alkaline phosphatase from Escherichia coli doc
... Dimer asymmetry and the catalytic cycle of alkaline phosphatase from Escherichia coli Stjepan Orhanovic ´ and Maja Pavela-Vranc ˇ ic ˇ Department of Chemistry, Faculty of Natural Sciences, Mathematics ... relating to the catalytic mech- anism of alkaline phosphatase (APase, E.C. 3.1.3.1), con- cern the influence of conformational changes and allosteric interactions on cata...
Ngày tải lên: 21/02/2014, 00:20
Báo cáo khoa học: Conserved residues in the N-domain of the AAA+ chaperone ClpA regulate substrate recognition and unfolding pdf
... specifically probe N -domain function. The RR ⁄ AA mutant not only displays normal basal ATPase activity but also retains the ability to interact with both ClpS and ClpP and most importantly is able ... contrast, SsrA-tagged GFP only stimulated the ATPase activity of wild-type ClpA (Fig. 3E). Together, these data suggest that RR ⁄ AA has a reduced ability to initiate unfolding of...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: Local changes in the catalytic site of mammalian histidine decarboxylase can affect its global conformation and stability pptx
... Y. , Pejler,G.,Tchougonova,E.,Hellman,L.,Gutsenstein,M., Hirasawa,N.,Sakurai,E.,Buzas,E.,Kovacs,P.,Casaba,Gy, Kittel ,A. ,Okada,M.,Hara,M.,Mai,L.,Numayama-Tsuruta, K.,Ishigaki-Suzuki,S.,Ohuchi,K.,Ichikawa ,A. ,Falus ,A. , Watanabe, T. & Nagy, A. (2001) ... [21]. In fact, the catalytic steps after the first transaldimination are rate-limiting in the entire catalytic reaction of...
Ngày tải lên: 08/03/2014, 08:20
Báo cáo khoa học: Functional symmetry in the isolated domain demonstrated by N-ethylmaleimide labelling pdf
... NBDs are capable of ATP hydrolysis [4], that inhibition of hydrolysis at one NBD effectively abrogates hydrolysis at the other [5], andthathydrolysisatthetwoNBDsmayoccurinan alternative fashion ... NBDs of human P-glycoprotein in an in vitro system to determine if asymmetry of NBDs can be detected in studies of the isolated domain. We investigate asymmetry at the initial st...
Ngày tải lên: 08/03/2014, 08:20
Tài liệu Báo cáo khoa học: Crystal structure of importin-a bound to a peptide bearing the nuclear localisation signal from chloride intracellular channel protein 4 ppt
... conformation. Database Structural data are available in the protein Data Bank under the accession number 3OQS. Structured digital abstract l CLIC4 and importin alpha bind by x-ray crystallography ... control at macromolecular crystallogra- phy beamlines. J Synchrotron Radiat 9, 401–406. 35 Leslie AG (2006) The integration of macromolecular diffraction data. Acta Crystallogr D: Biol...
Ngày tải lên: 14/02/2014, 19:20
Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf
... and catalysis. Biochemistry 48, 3417–3424. 31 Nakamura T, Matsumura H, Inoue T, Kai Y, Uegaki K, Hagihara Y, Ataka M & Ishikawa K (2005) Crystallization and preliminary X-ray diffraction analysis ... an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1. DNA Res 6, 83–101. 11 Nakamura T, Yamamoto T, Inoue T, Matsumura H, Kobayashi A, Hagihara Y, Uegaki K, Ataka M, Kai Y &a...
Ngày tải lên: 14/02/2014, 22:20
Tài liệu Báo cáo khoa học: Crystal structure of Klebsiella sp. ASR1 phytase suggests substrate binding to a preformed active site that meets the requirements of a plant rhizosphere enzyme doc
... active-site motifs, RHGXRXP and HD, and hydrolyze metal-free phytate with pH optima in the acidic range. They consist of two domains, a large a ⁄ b domain and a small a domain with the catalytic ... coli phytase, glucose- 1-phosphatase and human prostatic-acid phosphatase. The polypeptide chain is organized into an a and an a ⁄ b domain, and the active site is l...
Ngày tải lên: 16/02/2014, 09:20
Tài liệu Báo cáo khoa học: Crystal structure of the catalytic domain of DESC1, a new member of the type II transmembrane serine proteinase family pptx
... and the human airway trypsin (HAT) ⁄ HAT-like ⁄ DESC subfamily. Members of the hepsin and matriptase subfamilies are known structurally and here we present the crystal structure of DESC1 as a ... markedly from that of other struc- turally known TTSPs. Based on surface analysis, we propose a rigid domain association for the N-terminal SEA domain with the back site...
Ngày tải lên: 19/02/2014, 00:20