Báo cáo Y học: Protein methylation as a marker of aspartate damage in glucose-6-phosphate dehydrogenase-deficient erythrocytes docx

... Protein methylation as a marker of aspartate damage in glucose-6-phosphate dehydrogenase-deficient erythrocytes Role of oxidative stress Diego Ingrosso 1,2 , Amelia Cimmino 1 , Stefania D’Angelo 1 , ... the circulation [6,25,26]. This has been related to an increased number of abnormal aspartate residues, spontaneously arising from L -asparaginyl deamidation and/o...

Ngày tải lên: 08/03/2014, 22:20

... containing DNA as a MTase inhibitor. 2-pyrmidinone incorporation in DNA sequences may also serve as a specific probe for studying discrimination contacts formed by proteins and functional groups of pyrimidine ... proximity of the cofactor [2]. Several cytosine analogues, 5-fluorocytosine (FC), 5-azacytosine (AzaC) and 2-pyrimidinone (2P), have been reported as mechanism-based in...

Ngày tải lên: 07/03/2014, 15:20

... immunopre- cipitation of Tyr201Cys substituted ASA with mAb C. Degradation of amino acid-substituted ASAs via the proteasome In order to investigate the degradation pathway of amino acid-substituted ASAs in ... caused by the deficiency of arylsulfatase A (ASA). This enzyme catalyses the first step in the degradation pathway of the glycosphingo- lipid 3-O-sulfogalactosylceramid...

Ngày tải lên: 07/03/2014, 17:20

... subcellular U937 fractions. Cystatin F concentrations were measured with a specific ELISA, total protein concentration was measured with Coomassie binding assay and b-hexosaminidase activity was measured ... cystatin F and ER the staining was performed on cells cultured for 2 days. Cystatin F was stained as described above and after the last washing step 20 lgÆmL )1 Texas Red conjugated...

Ngày tải lên: 21/02/2014, 01:21

... recovered differentially based on varying stabilities. Remarkably, we have found that, at least in some circumstances, a quanti- tative correlation to biophysical data can be obtained from a statistical analysis ... the association of the variable heavy chain (V H )with protein A was used as a surrogate for direct stability measurements. The V H domains in camelid heavy chai...

Ngày tải lên: 19/02/2014, 12:20

... within lipofuscin-loaded lysosomes. Apparently, cells have a limited capacity to produce lysosomal enzymes, and as lipofuscin- containing lysosomes act as sinks for newly synthesized enzymes, they are not available ... jeo- pardize the stability of lysosomal membranes, resulting in leak of a variety of hydrolytic enzymes into the cytosol. Such lysosomal enzymes may directly ac...

Ngày tải lên: 17/03/2014, 23:20

... fibritin XN that had no C-terminal domain. Our goal was to answer a question about how many thermodynamically independent domains fibritin has, and to assign the thermal transitions to individual ... and F proteins were additionally applied to a 15-mL DEAE–Sephacryl column and eluted w ith a linear gradient o f NaCl. Fractions containing proteins were dialysed against Tris/EDTA buffer and...

Ngày tải lên: 24/03/2014, 03:21

... Kobayashi 2 , Yasuo Igarashi 3 and Jun Hasegawa 4 1 Graduate School of Biosphere Sciences, Hiroshima University, Japan; 2 Faculty of Pharmaceutical Science, Osaka University, Japan; 3 Department ... factors that affect protein stability, we attempted to achieve maximal enhancement of the stability of PA c-551 by introducing minimal mutations into spa- tially separate regions. Five a...

Ngày tải lên: 24/03/2014, 03:21

... perform a vaginal hysterectomy that was beyond the capacity of the registrar in question. As a result of poor surgical skills, the patient’s fallopian tube was entrapped in the suture line and later ... accepting the fee, the doctor has a contractual duty to provide a Abstract Medical practitioners have a duty to maintain a certain standard of care in providing...

Ngày tải lên: 25/10/2012, 10:02

... function in a gen- eral adult population. Thus, our findings may serve as a basis for experimental testing, as for example by adding measurement of respiratory functions to outcomes of ‘hypertensive’ ... measurements were taken with a 3-minute pause after a rest of at least 5 minutes in a sitting position on the right arm. The mean of the last two measurements was us...

Ngày tải lên: 25/10/2012, 10:45