Báo cáo Y học: Differential regulation of the Fe-hydrogenase during anaerobic adaptation in the green alga Chlamydomonas reinhardtii pot

... the Netherlands. 1032 T. Happe and A. Kaminski (Eur. J. Biochem. 269) Ó FEBS 2002 Differential regulation of the Fe-hydrogenase during anaerobic adaptation in the green alga Chlamydomonas reinhardtii Thomas ... directly to the H-cluster. Protein sequencing of the enzyme and recombinant expression of HydA in E. coli To verify that the hydA ORF encodes...

Ngày tải lên: 08/03/2014, 22:20

... Alternatively, hTR and hTERT may play a minimal catalytic activity in telomerase, while the assembly of other telomerase subunits may amplify the enzyme function. In this scenario, deletion of mTEP1 in embryonic ... of dyskerin, although showing comparable inhibition of telomerase function in cells, exhibited a weaker inhibition of telomerase activity in the in vit...

Ngày tải lên: 17/03/2014, 23:20

... inversely correlate with FAAH activity in peripheral lymphocytes [40]. The mechanism of F AAH activation b y leptin and progesterone was further investigated. Binding of leptin to the l ong isoform of ... they do not in human neuronal cells. Therefore, the central actions of these two hormones do not seem to involve the endocannabinoid system but instead may play a role...

Ngày tải lên: 30/03/2014, 15:20

... sense or use oxygen. The intracellular concentration of heme is maintained by the rate of its synthesis and degrada- tion [1]. Many enzymes and their regulators are responsible for heme synthesis [1,2]. ... d-aminolevu- linic acid dehydratase, the second enzyme of the heme biosynthetic pathway. SnPP is a competitive inhibitor of HO activity [35]. The heme content of all th...

Ngày tải lên: 19/02/2014, 05:20

... the exception of the temperature- activity profile, the characteristics of the enzyme were determined from initial velocity measurements in the direction of the NADH-dependent dihydroxyacetone phosphate ... any amino acid [19,20]. In contrast, some NADP + binding sites have an a lanine at the position c orresponding to the third glycine residue of the conserved t...

Ngày tải lên: 22/02/2014, 04:20

... transport of IL-6R through the secretory pathway [32]. Secondly, we avoided the use of a synthetic polypeptide linker in order to minimize immun- ogenicity. Instead, the 16 C-terminal amino acids of CNTF-R ... differences in physiological response in neur- onal cells. The underlying molecular mechanism might include the recruitment of the transducing proteins through...

Ngày tải lên: 22/02/2014, 07:20

... synthetase, inhibition of CoA-IT results in an increase in free AA and a corresponding build-up of AA in triacylglycerols that can be prevented by co-incubation of cells with CoA-synthetase inhibitors ... PAF [55].Thus,whilePLA 2 isotypes may not be directly involved in generating lyso PAF, the activity of PLA 2 on PtdEtn may indirectly drive the remodeling process that ge...

Ngày tải lên: 22/02/2014, 07:20

... effective inhibitors of recombinant CTE-I and MTE-I. The experi- ment using inhibitors of acyl-CoA synthetases in combina- tion with NDGA demonstrates that the two enzymes, the synthetase and the acyl-CoA ... synthesis in Y1 cells was significantly inhibited by a synergistic effect of NDGA and triacsin C, an inhibitor of the AA-CoA synthetase. Our results are consistent...

Ngày tải lên: 08/03/2014, 09:20

... liver. Furthermore, we investigated the speci®city of the human CBG with r espect to the glycone and aglycone moieties, and in particular characterized the ef®ciency of the enzyme in hydrolysing a ... reduced by 98% in the presence of 4NP-b- D -mannopyranoside (10 m M )]. Hydrolysis of xenobiotic glycosides by reCBG The a bility of CBG to hydrolyse a variety of...

Ngày tải lên: 08/03/2014, 16:20

... activate HS3 directly. One model for the globin chromatin opening proposes that factor binding at HS3 initiates the process, allowing the spreading of open chromatin, binding of other trans-acting factors ... recently been exten- sively reviewed [52–54]. The b-globin gene is part of the globin cluster, the genes of which are arranged in the order of their expression...

Ngày tải lên: 08/03/2014, 22:20