... antigen-binding affinity For the quantitative evaluation of the H39 mutation,kinetic analysis for antigen–Fv interaction of the wild-type and H39KQ mutant of purified Fv and scFvproteins was performed using ... (94 °C for 5 min, followed by sevencycles of 94 °C for 30 s, 55 °C for 30 s, and 72 °C for 1 min), followed by a normal cycling (30 cycles of 94 °C for 30 s, 55 °C for 30 s, and 72 °C for 1 min) ... absorbance, others showed adecreased or diminished antigen-dependency. To evalu-ate the OS-fitness of the clone, the ratio of the specificabsorbance in the presence of 10 lgÆmL)1HEL to thatin the...