... based on the concept of glycan profiling, and utilizes lectins, a group of glycan-discriminating proteins. In general, however, the glycan–lectin interaction is relatively weak in comparison with, ... analysis of glycans. In general, the current principal technologies in use are: (a) de novo sequencing; (b) glycan mass fingerprinting [29]; and (c) MS n spectral matching [2,30–32]....
Ngày tải lên: 16/02/2014, 08:20
... 6B). To determine the function of occludin, we reduced occludin expression in Huh7 cells by using occludin short hairpin RNA (shRNA). After determining the reduced expression level of occludin with ... facilitates the initiation of apop- tosis [22]. In addition, elevated calcium levels contribute to cell invasion [23,24]. In contrast, in the case of occludin, an ER-mediated in...
Ngày tải lên: 18/02/2014, 18:20
Tài liệu Báo cáo khoa học: Hypoxia reduces the expression of heme oxygenase-2 in various types of human cell lines A possible strategy for the maintenance of intracellular heme level pdf
... HO-1 and HO-2 expression in human cell lines We initially analyzed the effects of hypoxia on the expression of HO-1 and HO-2 in human cell lines of bone marrow origin, including KG1 myeloid cells, ... [3–5]. Characteristically, human HO-1 contains no cysteine residue [6], whereas HO-2 contains at least two copies of a potential heme-binding site, consisting of the cysteine and p...
Ngày tải lên: 19/02/2014, 06:20
Tài liệu Báo cáo khoa học: A FYVE-containing unusual cyclic nucleotide phosphodiesterase from Trypanosoma cruzi docx
... submitted]. Inhibitor profiling To explore the sensitivity of TcrPDEC against a spec- trum of inhibitors, the potency of a number of com- mercially available PDE inhibitors was determined using 1 lm ... poly- peptide chain (amino acids T 291 –S 657 of a total of 924). The C-terminal portion contains no recognizable func- tional domains or motifs. In the amino acid sequence of t...
Ngày tải lên: 19/02/2014, 07:20
Tài liệu Báo cáo khoa học: A novel c-N-methylaminobutyrate demethylating oxidase involved in catabolism of the tobacco alkaloid nicotine by Arthrobacter nicotinovorans pAO1 ppt
... isoalloxazine ring (Fig. 5B, broken line, number 3). Phe in place of Trp66 r esulted in a protein with noncovalently bound FAD, again showing no enzyme activity, and isolation of the flavin cofactor ... only in bacteria grown in the presence of nicotine The DNA carrying the sarcosine dehydrogenase-like ORF63, corresponding to a protein of 813 amino acids with a predicted molecu...
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo khoa học: "A Finite-State Model of Human Sentence Processing" docx
... convincing since each pair of sentences in the experiment share the exactly same set of words except their order. 4.2 Probability Decrease at the Disambiguating Region A total of 30 pairs of a ... run- ning probability at the beginning of a sentence will be 1, and will keep decreasing at each word break since it is a product of conditional probabilities. We tested the predictabi...
Ngày tải lên: 20/02/2014, 11:21
Tài liệu Báo cáo khoa học: "A semantically-derived subset of English for hardware verification" pdf
... involve non- compositional indirect semantics when there is some aspect of non-locality in the domain of the translation function. That is, some form of inference is required probably involving ... holding at a state being the letters contained in the circle. A CTL structure gives rise to an infinite computation tree, and Figure 2 3Subsequently, model-checking methods which...
Ngày tải lên: 20/02/2014, 19:20
Tài liệu Báo cáo khoa học: A selenium-containing single-chain abzyme with potent antioxidant activity docx
... Enzymology and Engineering of Ministry of Education, Jilin University, Changchun, P. R. China; 2 Key Laboratory for Supramolecular Structure and Materials of Ministry of Education, Jilin University, ... chemically synthesizing a GPX mimic: one in which the selenium atom binds directly to a heteroatom such as nitrogen Correspondence to G. Luo, Key Laboratory of Molecular Enzymology...
Ngày tải lên: 21/02/2014, 00:20
Báo cáo khoa học: Proteolytic activation and function of the cytokine Spatzle in the innate immune response of a lepidopteran insect, Manduca sexta ppt
... residue cystine-knot domain. Injection of Spa ¨ tzle-C108, but not proSpa ¨ tzle-1A, into larvae stimulated expression of several antimicrobial peptides and proteins, including attacin-1, cecropin-6, moricin, lysozyme, ... contained a 30 bp insertion in the ORF, beginning at nucleotide 516. This resulted in insertion of a 10 amino acid segment (TREIDYPETI) and one substitution (Ser...
Ngày tải lên: 06/03/2014, 09:22
Báo cáo khoa học: A Caenorhabditis elegans model of orotic aciduria reveals enlarged lysosome-related organelles in embryos lacking umps-1 function potx
... or the persistence of intestinal vacu- oles in umps-1(zu456) larvae (data not shown), sug- gesting that R12E2.11 does not play a major role in regulating the size of intestinal organelles. Moreover, R12E2.11(RNAi) ... the indicated strain was grown on plates containing E. coli expressing dsRNA against the listed gene. g Embryos from parents containing extrachromosomal arrays were scor...
Ngày tải lên: 06/03/2014, 09:22