... M.Jha for the synthesis of compounds 4, 6, 8, 12–15, 18and 20. We acknowledge the technical assistance of P.B. Chumala, Department of Chemistry, for develop-ment of the HPLC method B used for ... curves of BHs. (A) Brassinin saturationcurve for BHLmL2 and (B) brassinin saturation curve for BHAb. Themixture was incubated at 23 °C for 45 min in the presence of increasing concentrations of ... with55 mL of the same buffer, first alone and then with a200 mL of a decreasing gradient of ammonium sul-phate in 20 mm Tris⁄ HCl buffer (pH 8.0) with a flow of 0.8 mLÆmin)1. Fractions of 2.5...