... for Saccharomyces carlsbergensis, no data available for E. coli;bvalue for Lupinus luteus and bovine, no data available for E. coli;cvalue for Paracoccus denitrificans, no data available for ... hCypA labelling were 50 lM for [15N]Trp and[15N]Tyr, 150 lM for [15N]Ile, [15N]Thr and [15N]His,0.35 mM for remaining Group II [15N]amino acids, and1mM for those in Group III. ... acids and s uitable for analysis by NMR spectroscopy without chromatographicpurification. A system b ased on an Escherichia c oli cell ex-tract was optimized with regard to protein y ield and...