Báo cáo Y học: Spectroscopic characterization and ligand-binding properties of chlorite dismutase from the chlorate respiring bacterial strain GR-1 ppt

... we present the EPR spectroscopic and redox properties of GR-1 chlorite dismutase. Furthermore, we investigated the binding of hydroxide and imidazole to the ferric enzyme and of NO to the ferrous enzyme ... oxoferryl species and hypochlorite. The reaction mechanism of WF10 with hemoproteins may be similar to the enzymatic reaction of chlorite with chl...

Ngày tải lên: 08/03/2014, 16:20

... clustered together and distant from IL-20 and IL- 24, as recently determined from analysis of the spotted green pufferfish genome, imply that the carp sequence is IL-10. The hydropathy analysis also ... IL-10 inhibits the activation of macrophages/monocytes, thereby inhibiting cytokine synthesis, nitric oxide (NO) production and the expression of other costimulatory m...

Ngày tải lên: 20/02/2014, 02:21

... sequence analysis of HpSDH In the current work, the aroE gene of H. pylori strain SS1 was cloned by using the genome sequences of H. pylori strains 26695 and J99 as major references. We firstly amplified ... result. The low catalytic effi- ciency of HpSDH may result from the sequence vari- ation in the binding sites of substrate and cofactor. However, in light of...

Ngày tải lên: 19/02/2014, 05:20

... the locations of the four reflections (see text). Ó FEBS 2003 Biophysical study of Mycoplasma glycolipid (Eur. J. Biochem. 270) 3275 LAL assay The LAL test is based on the property of lysates of amebocytes ... molecules. Gel-to-liquid crystalline phase transition and lipid headgroup conformations The determination of the b«a gel-to-liquid crystalline phase transition o...

Ngày tải lên: 21/02/2014, 00:20

... [23]. Zymogen conversion to the active enzyme occurs by limited proteolysis of the inhibitory pro-sequence segment and may be either autocatalytic or involve acces- sory molecules. The length of ... and a polyethylene glycol polysty- rene support with a 5-(4¢-Fmoc-aminomethyl-3¢-5¢-dimeth- oxyphenoxy)valeric acid linker. After synthesis, peptides were purified by reverse-phase HP...

Ngày tải lên: 08/03/2014, 16:20

... Genotype H was recently identified in individuals from Central America and California [2]. Several genotypes may be associated with the severity of the disease but the relationship between the ... mediated by point mutations with the YMDD motif at the catalytic center of the viral reverse transcriptase. With increase in the mutant viral load, patients can sustain fu...

Ngày tải lên: 02/11/2012, 11:17

Ngày tải lên: 03/11/2012, 11:11

... map and glycan analysis of the natural b-fructofuranosidase Investigation of the carbohydrate moieties of the purified allergen from tomato was carried out by MALDI-TOF mass spectrometry. From the ... 2003 glycan analysis of the natural protein from tomato revealed that MMXF is the dominating glycan with about 84% of all sugar structures. The structures of th...

Ngày tải lên: 21/02/2014, 00:20

... Analysis of the promoter region of the cystatin F gene (CST7) showed that it, like that of the cystatin C gene (CST3), is devoid of typical TATA- and CAAT-box ele- ments. In contrast to the cystatin ... band (lane 3, band a)withM r approximately 3200 Da higher than the diglycosylated cystatin F species. The nature of this band is presently unknown and is discussed be...

Ngày tải lên: 21/02/2014, 01:21

... 8). Correspondingly, fluorescence of cytoplasmic T4(259–470)– YFP and full-length T4–GFP was already significantly reduced after 2 min of bleaching in a small area of the cytoplasm, whereas the fluorescence of ... transiently transfected with the respective expression plasmids and analyzed by confocal microscopy the next day. Full-length GFP-tagged TRAF4 molecules were local...

Ngày tải lên: 21/02/2014, 15:20