Báo cáo Y học: Identification of the 19S regulatory particle subunits from the rice 26S proteasome potx
Tài liệu Báo cáo Y học: Identification of a set of genes involved in the biosynthesis of the aminonucleoside moiety of antibiotic A201A from Streptomyces capreolus pdf
... be related to their counterparts of the puromycin and hygromycin A biosynthetic pathways, respectively. The puromycin biosynthetic gene cluster (pur )from S. alboniger is partially characterized. ... GDP- D -mannose and other hexose dehydratases (data not shown) (Fig. 3). A variety of these enzymes synthesize 4-keto-6-deoxy-GDP- D -mannose, a key intermediate in the biosynthesis o...
Ngày tải lên: 21/02/2014, 01:21
Báo cáo Y học: Identification of the 19S regulatory particle subunits from the rice 26S proteasome potx
... homology to RP subunits. Ó FEBS 2002 The 19S regulatory particle from rice (Eur. J. Biochem. 269) 1479 Table 3. Identification of the rice 19S regulatory particle subunits by ESI-Q-TOF MS. myri, N-myristoylaton; ... subunits S4 and S7. J. Biol. Chem. 275, 875–882. Ó FEBS 2002 The 19S regulatory particle from rice (Eur. J. Biochem. 269) 1483 Identific...
Ngày tải lên: 08/03/2014, 16:20
Báo cáo Y học: Identification of residues in the PXR ligand binding domain critical for species specific and constitutive activation docx
... closely with the crystal structure. There are other parts of the model that do not correlate with the crystal structure, in particular the region Fig. 4. A diagram of basal transcriptional activity ... protein content of the nuclear and cytoplasmic fractions were determined by amino-acid analysis. 250 lL of 6 M HCl with 0.5% Phenol was added to each of the samples (5 lL)...
Ngày tải lên: 08/03/2014, 16:20
Báo cáo Y học: Identification of residues critical for activity of the wound-induced leucine aminopeptidase (LAP-A) of tomato pptx
... and the carbonyl oxygen of the P1 residue, Zn1 is bound by the two carboxyl oxygens of Asp25 5 (Asp347) and a car boxyl ox ygen of Glu334 (Glu429). T he interact ions of Zn1 and the carboxyl oxygen of ... (Asp366), the carbonyl oxygen of the P1 residue with the side-chain amine of Lys262 (Lys354), and the backbone amino group of the P 1¢ residue with the ba...
Ngày tải lên: 08/03/2014, 23:20
Báo cáo Y học: Identification of mammalian-type transglutaminase in Physarum polycephalum Evidence from the cDNA sequence and involvement of GTP in the regulation of transamidating activity potx
... nucleotides inhibit the enzymatic activity of PpTGase might be similar to that by which they inhibit the enzymatic activity of TGase 4. Hydrolysing activity of GTP was also found in the purified PpTGase ... m M Ca 2+ , the enzymatic activity was apparently decreased by the addition of 100–500 l M GTP. In the presence of 1 m M Ca 2+ , an inhibitory effect was observed only...
Ngày tải lên: 17/03/2014, 23:20
Báo cáo Y học: Identification of a critical lysine residue at the active site in glyceraldehyde-3-phosphate dehydrogenase of Ehrlich ascites carcinoma cell ppt
... enzyme of the glycolytic pathway, may play a primary role in the high aerobic glycolysis of malignant cells [2 –11]. These investigations have indicated a strong enhancement of expression of ... measuring the enzyme activity. For both the enzymes, control tubes were maintained at the respective pH values. Percentage activity was calculated by assuming the activity of th...
Ngày tải lên: 24/03/2014, 04:21
Báo cáo y học: Identification of an element within the promoter of human selenoprotein P responsive to transforming growth factor-b ppt
... repress the transcriptional activity of promoters bearing SBEs. In addition, we showed very recently the involvement of TGIF, Smads and an SBE in the down- regulation by TGF-b of the human aryl hydrocarbon receptor ... species assessed is the mRNA (i.e. the respective cDNA) of SeP, the latter assay gauges the activity of an enzyme derived from an unrelated mRNA, na...
Ngày tải lên: 31/03/2014, 23:20
Tài liệu Báo cáo Y học: Identification of syntaxin-1A sites of phosphorylation by casein kinase I and casein kinase II ppt
... identify the site(s) of syntaxin-1A that a re phospho- rylated by CKIa, constructs 1–190 and 1–265 of syntaxin-1A were phosphorylated by CKI a and subjected to trypsin digestion. The tryptic peptides ... on phosphopeptides from each of the constructs. Because the site of phosphorylation by CKII was close to the N-terminus of syntaxin-1A, automated Edman degra- dation of...
Ngày tải lên: 22/02/2014, 04:20
Báo cáo Y học: Identification of novel membrane proteins by searching for patterns in hydropathy profiles potx
... designed for the acetylcholine (ACh) and glycine receptors of the cys-loop receptor superfamily. The key feature was a closely spaced triplet of hydropathy peaks bracketed by deep valleys. When applied ... (A) The hydropathy profile of a rat dopamine symporter reveals a pair of peaks followed by a deep valley, then a cluster of nine peaks. The peak, base and valley threshol...
Ngày tải lên: 17/03/2014, 23:20
Tài liệu Báo cáo Y học: Kinetic study of sn-glycerol-1-phosphate dehydrogenase from the aerobic hyperthermophilic archaeon, Aeropyrum pernix K1 potx
... dramatically, which seemed to be caused by irreversible denaturation of the enzyme. With the exception of the temperature- activity profile, the characteristics of the enzyme were determined from initial ... specificity and enzyme activity The substrate specificity of Gro1P dehydrogenase was examined using the purified enzyme. No activity w as observed t oward glyceraldehy...
Ngày tải lên: 22/02/2014, 04:20