Báo cáo Y học: Regulation of a1,3galactosyltransferase expression in pig endothelial cells Implications for xenotransplantation doc

... Regulation of a1,3GalT expression in pig endothelial cells (Eur. J. Biochem. 269) 1473 Regulation of a1,3galactosyltransferase expression in pig endothelial cells Implications for xenotransplantation Dominique ... promoter of the long form of b1,4-gala ctosyl- transferase contains 12 Sp1 binding sites, and is active in a variety of cell types [42, 4...

Ngày tải lên: 08/03/2014, 16:20

... HA released into the culture medium was measured by the sandwich binding protein assay using hyaluronan binding protein (HABP) according to the manufacture’s instructions for the hyaluronic acid ... level of the has operon containing the hyaluronan synthase gene or the protein level of hyaluronan synthase. Solubilization of the enzyme from membranes of MU-treated cells and additio...

Ngày tải lên: 08/03/2014, 09:20

... glycosylphophatidylinositol (GPI) anchors [1,2]. Although the primary structure of the glypi- cans is only marginally conserved (about 30% identity), there is a strict conservation of 14 cysteine ... within the core protein leading to compact conformation but also of HS-attachment consensus sequences at the C-termini of proteins. Syndecans and glypicans are individually expressed in...

Ngày tải lên: 17/03/2014, 23:20

... role in progression of the cell cycle and the differentiation of cells [11,12]. The methylation of DNA plays a role in the regulation of gene expression [13,14], genomic imprinting [15] and inactivation ... (Fig. 5A,B). In contrast, the intensity of the shifted band that included GST–Sp1 gradually increased. Conversely, the addition of GST–Sp3 resulted in a similar r...

Ngày tải lên: 22/02/2014, 07:20

... glycoprotein VI; PtdIns3K, phosphatidylinositol 3-kinase; PVDF, poly(vinylidene difluoride); PKC, protein kinase C; PP1, (4-amino-4-(4-methylphe- nyl)-7-(t-butyl)pyrazola[3,4-d]pyrimidine); SH2, ... ERK activation induced by thrombin is dependent on SRC kinases SRC family kinases are necessary for the initial signalling events induced by GPVI, notably for phosphorylation of the G PVI-ass...

Ngày tải lên: 08/03/2014, 16:20

... implicated in the mammalian SAPK systems. REGULATION OF SAPK SIGNAL PATHWAYS BY PROTEIN PHOSPHATASES IN YEAST CELLS A molecular g enetic analysis of ye ast cells indicated that two distinct protein phosphatase ... phosphorylation of conserved tyrosine and threonine residues within the catalytic domain. This phosphorylation is mediated by dual specificity protein kinases, member...

Ngày tải lên: 17/03/2014, 23:20

... members of main protein kinase superfamily, Ryazanov coined the term Ôa-kinaseÕ for this unusual group of enzymes. Further discussion of this family may be found in a recent review by Ryazanov ... translo- cation and in poly(U)-directed polyphenylalanine synthesis [26,27], by preventing it from binding to the ribosome [28]. Early data showed that the phosphorylation of eEF2 was...

Ngày tải lên: 23/03/2014, 21:20

... addressed by exploring the effect of rapamycin on the control of overall rates of protein synthesis. In cell lines, rapamycin generally exerts only a small inhibitory effect on the rate of protein synthesis ... (1997) Regulation of protein kinase B and glycogen synthase kinase 3 by insulin and b-adr- energic agonists in rat epididymal fat cells. Activation of protein kinase...

Ngày tải lên: 23/03/2014, 21:20

... dephosphorylation is mediated by calcineurin, rephosphorylation is catalyzed by a variety of serine kinases, such as glycogen synthase kinase- 3, ERK, p38, casein kinase-2, and c-Jun N-terminal kinase [40–43]. ... [3–5]. Thus, the elimination of highly activated T -cells by the Fas/ FasL system is critical for the downregulation of immune responses, the homeostasis of lymphocytes,...

Ngày tải lên: 24/03/2014, 03:21

... within the initial 20 min following the addition of 14 C-labelled taurine. Using taurine uptake within the initial 20 min in NaCl medium containing extracellular taurine (0–50 l M ) and fitting ... NF-jB and for tonicity response element binding protein (Ton-EBP) [10–12]. The activity of p53 is regulated by a series of protein phosphorylations, acetylations and glycosylations of pa...

Ngày tải lên: 30/03/2014, 15:20