Báo cáo Y học: Molecular cloning, bacterial expression and properties of Rab31 and Rab32 docx
Báo cáo Y học: Molecular cloning, bacterial expression and properties of Rab31 and Rab32 docx
... m ay be a long form of Rab32 (see Discussion). Bacterial expression of Rab31 and Rab32 GST Rab31, GST Rab32 and the potentially GTPase- de®cient mutants of t hese proteins, GST Rab31 Q64L and GST±R ... membrane [45]. Binding of guanine nucleotides and hydrolysis of GTP by GST Rab31 and GST Rab32 Many studies have shown that bacterially expressed R ab proteins...
Ngày tải lên: 08/03/2014, 16:20
Tài liệu Báo cáo khoa học: Molecular cloning, recombinant expression and IgE-binding epitope of x-5 gliadin, a major allergen in wheat-dependent exercise-induced anaphylaxis ppt
... QQFPQQQ, QSPEQQQ, YQQYPQQ and QQFHQQQ. Among these IgE- binding epitope sequences, QQPPQQ, YQQYPQQ, QSPEQQQ and QQFHQQQ, were newly detected in this study. Molecular cloning and sequence of x-5 gliadin gene Many ... three peaks of molecular mass 49 085, 50 300, and 51 500 by MALDI-TOF MS [9]. However the calculated mole- cular mass (50 900) of x-5a gliadin did not coincide w...
Ngày tải lên: 20/02/2014, 01:20
Báo cáo Y học: Molecular cloning and characterization of isomultiflorenol synthase, a new triterpene synthase from Luffa cylindrica, involved in biosynthesis of bryonolic acid ppt
... oxidosqualene cyclase cDNA, LcIMS1, was isolated from cultured cells of Luffa cylindrica Roem. by heterologous hybridization with cDNA of Glycyrrhiza glabra b-amyrin synthase. Expression of LcIMS1 in yeast lacking ... whole growth cycle. These results suggest independent regulation of these two genes and of the accumulation of bryonolic acid and phytosterols. Keywords: bryon...
Ngày tải lên: 08/03/2014, 23:20
Tài liệu Báo cáo Y học: Short peptides are not reliable models of thermodynamic and kinetic properties of the N-terminal metal binding site in serum albumin doc
... Bal 1,2 1 Faculty of Chemistry, University of Wroclaw, Poland; 2 Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland A comparative study of thermodynamic and kinetic ... Asp- Ala-His-NH 2 (DAHam)wasagiftofHenrykKozlowski, Faculty of Chemistry, University of Wroclaw. Stock solutions of NiCl 2 and CuCl 2 were standardized gravimet- rically...
Ngày tải lên: 22/02/2014, 04:20
Báo cáo khoa học: Molecular cloning, expression and characterization of protein disulfide isomerase from Conus marmoreus pdf
... h, and then the lyso- zyme activity was measured. The refolding yields were calculated from the activity recovery on the basis of a standard curve. Fig. 3. The amino acid sequences of tx3a and ... reached approximately 40%. At low concentrations, both cPDI and hPDI decreased the refolding yield of lysozyme (antichaperone activity). At high concentrations, both cPDI and hPDI incr...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: Molecular cloning, expression analysis and functional confirmation of ecdysone receptor and ultraspiracle from the Colorado potato beetle Leptinotarsa decemlineata pdf
... WSNGGNTAYCAYTAYAAYGC LdUSP-F1 ATHTGYGGNGAYMGNGC LdEcR-F2 GARGGNTGYAARGGNTTYTT LdUSP-F2 GGNAARCAYTAYGGNGTNTA LdEcR-F3 TGMGNMGNAARTGYCARGARTG LdUSP-R1 TCYTCYTGNACNGCYTC LdEcR-R1 TCNSWRAADATNRCNAYNGC LdEcR-R2 ... (1999) Expression of ecdysone receptor isoforms and trehalase in the anterior silk gland of Bombyx mori during an extra larval molt and precocious pupation induced by 20-hydro...
Ngày tải lên: 07/03/2014, 21:20
Báo cáo khoa học: Molecular cloning, expression and characterization of cDNA encoding cis-prenyltransferases from Hevea brasiliensis A key factor participating in natural rubber biosynthesis pdf
... numbers AB061234 and AB064661 respectively. The ORFs of HRT1 and HRT2 encode 290 and 284 amino acid residues (87.3% identity) with predicted molecular masses of 33.2 and 32.8 kDa respectively. Hydropathy and ... 4673 intervals, and assayed for radioactivity. The molecular mass of the reaction products were estimated by comparing them with the elution volumes of commerc...
Ngày tải lên: 07/03/2014, 21:20
Báo cáo Y học: Molecular mechanisms underlying SHP-1 gene expression potx
... activity in SKOV3 cells. In our EMSA analyses, aside from the shifted band that contained USF1 and USF2, we observed other shifted bands (X and Y) . As bands X and Y were only partially inhibited by ... & Molecular Biology, Toronto Western Research Institute, University Health Network, Toronto, Ontario, Canada; 2 Laboratory of Gynecologic Oncology, Department of Obstetric G...
Ngày tải lên: 08/03/2014, 23:20
Báo cáo y học: "Molecular Virology of Hepatitis C Virus (HCV): 2006 Update"
... understanding of the molecular biology of hepatitis C virus (HCV) was achieved recently. The replicon system revolutionized the investigation of HCV RNA replication and facilitated drug discovery. ... discovery. Novel systems for functional analyses of the HCV glycoproteins allowed the validation of HCV receptor candidates and the investigation of cell entry mechanisms. M...
Ngày tải lên: 02/11/2012, 10:00
Tài liệu Báo cáo khoa học: Molecular cloning and characterization of soybean protein disulfide isomerase family proteins with nonclassic active center motifs pdf
... The amounts of pro-b-conglycinin and proglycinin are considered to be nearly equivalent to the synthesis levels of both b-conglycinin and glycinin, as pro-b-conglycinin and proglycinin are transient ... folding and quality control of nascent polypeptides [25]. In soybean cotyledon, large amounts of seed stor- age proteins such as glycinin and b-conglycinin are synthesized a...
Ngày tải lên: 18/02/2014, 11:20