Báo cáo Y học: Functional expression of human liver cytosolic b-glucosidase in Pichia pastoris Insights into its role in the metabolism of dietary glucosides ppt

... G. Berrin et al. (Eur. J. Biochem. 269) Ó FEBS 2002 Functional expression of human liver cytosolic b-glucosidase in Pichia pastoris Insights into its role in the metabolism of dietary glucosides Jean-Guy ... it might have a role in human aging [6]. Finally, a b-glucosidase, termed cytosolic b-glucosidase, is present in the liver, kidney,...

Ngày tải lên: 08/03/2014, 16:20

... folding of the mutant. The lower intensity may arise from partitioning of the protein into an aggregated state. The effect of copper binding on the secondary struc- ture was determined using CD in ... protein. Typically an initial injection of 2 lL copper chelate solution was followed by a further 29 injections of 4 lL of Cu(II) into the protein in the sample...

Ngày tải lên: 19/02/2014, 06:20

... potential therapeutic approach when considering the reported high expression of the ankyrin-repeat oncoprotein (gankyrin) in human hepatocellular carcinoma . Gankyrin binds to the cell-cycle regulator ... gastrointestinal tract leading to increase in the permeability of the gut mucosa to endotoxins [43, 44]. These factors are involved in ethanol-induced liver...

Ngày tải lên: 31/10/2012, 15:28

... mechanism of globin m-RNA stability in human RBCs. Further experiments are needed to understand the mechanisms and the biological meaning of these findings. But gene expression profiling of human ... expression profiling of human erythrocyte could be an important key for under- standing the machinery of anucleate protein synthesis and its meaning in the...

Ngày tải lên: 03/11/2012, 10:58

... of cystatin F is hypothetical. Although most cystatins inhi- biting cathepsin L also inhibit cathepsin S, an inhibitory activity of cystatin F against cathepsin S has not yet been reported. The ... of the cystatin F gene and promoter. The structures of the human cystatin F (CST7)andcystatinC(CST3) genes are illustrated schematically at the top. In the upstream region...

Ngày tải lên: 21/02/2014, 01:21

... found in DM43 first three domains and are present in the inhibitory receptors of Fig. 6. Inhibition of in vitro cytotoxicity of myotoxins I or II by DM64. Cytotoxicity was analyzed in vitro using ... effectively neut- ralized the myotoxic and cytotoxic effects of both myotox- ins. Interestingly, DM64 did not inhibit the PLA 2 activity of myotoxin I nor its intracerebr...

Ngày tải lên: 21/02/2014, 01:21

... Alternatively, the B-ring hydroxylation of naringenin to (2S)- eriodictyol preceeding the 3b-hydroxylation yields the substrate (2R,3R)-dihydroquercetin. Both dihydrokaempferol and dihydroquerce- tin ... Germany) as required for the substitution of glycine by alanine and proline, respectively, or of proline by glycine (Table 1). Each individual mutation was verified by DNA sequen...

Ngày tải lên: 21/02/2014, 03:20

... shock/a-crystallin proteins, and a two or three domain structure is proposed for these proteins [7,8]. The a-crystallin domain, located toward the C-terminus of the protein monomer, consists of 80–100 ... of colony forming units (cfu) per mL were plotted against the length of heat shock in min. The results shown are the average of three independent experiments. Bacteri...

Ngày tải lên: 22/02/2014, 04:20

... under the control of the GAL10 pro- moter. The induction of the yeast, by galactose containing media, results in overexpression of GAL4, in turn inducing an increase of the expression of the OR ... Controls for the immunocytochemical reaction were carried out by replacing either the primary or the secondary antibody by the incubation buffer in the react...

Ngày tải lên: 07/03/2014, 16:20

... obtain a recombinant protein containing the C-myc epitope followed by six histidines at the C-terminus. A plasmid containing the wild type version of CCR5 was constructed by introducing the coding sequence ... plasmid. The resulting XhoI/ApaI fragment was cloned into the corres- ponding sites in the polylinker region of the pcDNA3.1- myc-His expression vector (Invitr...

Ngày tải lên: 08/03/2014, 09:20