Báo cáo khoa học: Isolation and enzymatic characterization o

Báo cáo khoa học: Isolation and enzymatic characterization of lamjapin, the ﬁrst ribosome-inactivating protein from cryptogamic algal plant (Laminaria japonica A) ppt

... Isolation and enzymatic characterization of lamjapin, the ﬁrst ribosome-inactivating protein from cryptogamic algal plant ( Laminaria japonica A) Ren-shui Liu 1 , Jia-hua Yang 2 and Wang-Yi ... as the distribution of this class of protein in plant kingdom. MATERIALS AND METHODS Materials The fresh tender leaves of kelp (Laminaria japoni...

Ngày tải lên : 08/03/2014, 10:20

7
479
0

Báo cáo khóa học: Overexpression and enzymatic characterization of Neisseria gonorrhoeae penicillin-binding protein 4 ppt

... urethra having a pH of 6.2–8.4. The pH dependence of NG PBP 4 (pK a values of 6.9 and 10.1) therefore overlaps the alkaline side of the physiological growth range of N. gonorrhoeae in both males and females, ... inhibit- ors, and pH dependence. Comparison of the enzymatic properties of NG PBP 4 with other DD -carboxypeptidases highlights both similarities and d...

Ngày tải lên : 23/03/2014, 12:20

10
328
0

Báo cáo khóa học: Isolation and biochemical characterization of two soluble iron(III) reductases from Paracoccus denitriﬁcans docx

... (in kDa) on the right. Fig. 2. N-Terminal amino acid sequences of P. denitriﬁcans FerA and FerB (A) and comparison of the N-terminal amino acid sequence of FerB with the sequences of other oxidoreductases ... column of Superose 12, the same column was used to estimate their molecular sizes. By calibration of the column with standard proteins of known molecular mas...

Ngày tải lên : 30/03/2014, 13:20

10
365
0

Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx

... A3(2). Cloning and expression of the putative hyd genes The deduced open reading frames of putative hyd genes were PCR-ampliﬁed from the genomic DNA of the corresponding organisms. The PCR fragments of the ... performed using T-COFFEE [44]. Docking simulation of substrate on the active site of HYD Js The substrate binding pockets of HYD Js and the mode o...

Ngày tải lên : 18/02/2014, 08:20

14
621
0

Tài liệu Báo cáo khoa học: Bioinformatic and enzymatic characterization of the MAPEG superfamily doc

... [57]. Isolation and cloning of the SynMGST and E.coliMGST The coding sequence for the SynMGST, corresponding to the complementary strand of the nucleotide sequence from 89 254 to 89 667 in the ... above and in [1]). We investigated representative gene products from the E. coli and Synechocystis sp. bacteria further, to gain insight into the function of thes...

Ngày tải lên : 19/02/2014, 17:20

16
524
0

Báo cáo khoa học: Isolation and structural characterization of the Ndh complex from mesophyll and bundle sheath chloroplasts of Zea mays pptx

... provides the proton motive force essential for the synthesis of ATP. Homologs of this complex exist in bacteria, the mitochondria of eukaryotes, and the chloroplasts of plants. The bacterial and mitochondrial Keywords chloroplast; ... (56 kDa), NdhE (12 kDa), NdhF (83 kDa), NdhG (18 kDa), NdhH (45 kDa), NdhI (21 kDa), NdhJ (18 kDa), NdhK (29 kDa). The 75, 51 and 23 k...

Ngày tải lên : 23/03/2014, 13:20

12
431
0

Báo cáo khoa học: Biochemical and structural characterization of mammalian-like purine nucleoside phosphorylase from the Archaeon Pyrococcus furiosus pptx

... to rescue the possible damage of the other two disulﬁde bonds. The presence of a low percentage of the protein with Cys254 and Cys256 in the reduced form further supports this hypothesis. It ... role in the stability of the enzyme against thermal inactivation. The characterization of the thermal properties of the C254S ⁄ C256S mutant suggests that the C...

Ngày tải lên : 23/03/2014, 09:21

14
384
0

Tài liệu Báo cáo khoa học: Structural and biochemical characterization of a human adenovirus 2/12 penton base chimera pptx

... 4339 containing the RGD motif, is located at the top of the distal domain within the first insertion, and faces the solvent-exposed exterior of the particle. As in the hAd2pb structures, even the shorter ... the K d for the initial binding of minifiber. The fluorescence emission of the TMR was assumed to be independent of the binding of the minifibe...

Ngày tải lên : 19/02/2014, 06:20

10
647
0

Tài liệu Báo cáo khoa học: Molecular and functional characterization of adenylate kinase 2 gene from Leishmania donovani pdf

... induction of the culture with IPTG, which matched the molecular mass predicted from the amino acid sequence of AK2 protein. The puriﬁcation and functional folding of L. donovani AK from the E. coli cell ... 270) 4345 that of E. coli AK. The ﬂexible LID domain which provides open and closed conformation to the protein during binding of the substrate is show...

Ngày tải lên : 21/02/2014, 00:20

9
487
0

Tài liệu Báo cáo khoa học: Biochemical and molecular characterization of hazelnut (Corylus avellana) seed lipoxygenases pdf

... providing the substrates for the activity of all the enzymes located downstream on the pathway, have a primary role in the biosynthesis of phytooxylipins and can affect their availability inside the plant ... products of the hazelnut LOX were compared with the reaction products of soybean LOX type I and authentic standards of 9- and 13-HODE. As shown in Fig....

Ngày tải lên : 21/02/2014, 00:20

11
404
0