Báo cáo khoa học: Role of Ca2+/calmodulin regulated signaling pathways in chemoattractant induced neutrophil effector functions Comparison with the role of phosphotidylinositol-3 kinase ppt

... multiple intracel- lular signaling pathways. Therefore, the role of calmodulin, the major downstream effector of Ca 2+ , in the regulation of intracellular signaling was analyzed by using the inhibitor W7. ... counteracts the calcineurin induced affinity switch of a 5 b 1 integrin in CHO cells, suggesting also a role for Ca 2+ /calmodulin-dependent molecules...

Ngày tải lên: 08/03/2014, 10:20

... 37% of its original size) is typically in the range 2–3 mm [26]. Finding experimental evidence that electrical cou- pling is the key ‘spring’ interlinking the Ca 2+ stores has involved repeating ... junctions could serve as the spring joining the pendulums in the above analogy. However, coupling through release of Ca 2+ results in very weak coupling, as the effective d...

Ngày tải lên: 16/02/2014, 09:20

... and P2X7 receptors, suggesting the involvement of multiple pathways in the release. In addition to these, the release of ATP is partly dependent on Ca 2+ [15] and SNARE proteins [16], and astrocytes ... events in the synchronization of SICs [31]. As shown in the right panel of Fig. 3B, reactive astrocytes increase the release of glutamate by facilitating Ca 2+...

Ngày tải lên: 16/02/2014, 09:20

... cytoplasmic domain of cadherin interacts with F-actin via b-catenin and a-catenin; b-catenin binds to cadherin and a-catenin, which in turn interacts with F-actin (Fig. 3A) [24]. Thus b-catenin plays ... pivotal role in the regulation of cell–cell adhesion. The interac- tion of b-catenin with cadherin is regulated by tyrosine phosphorylation of b-catenin [25,26], whi...

Ngày tải lên: 16/02/2014, 09:20

... kept in serum-containing medium instead of Ringer solution [16]. Interestingly, the gene encoding TRPM4 has been shown to be one of the genes upregulated during the transition from prostatic intraepithelial ... alteration in Ca 2+ entry follow- ing store depletion. In uence of TRPM4b on ionomycin -induced membrane depolarization Ca 2+ entry induced by depletion of intrac...

Ngày tải lên: 23/03/2014, 09:21

... understanding of the role of various interacting components. The formation of dopamine–quinone adducts (because of auto-oxidation of the neurotrans- mitter), especially dopaminochrome, with a-synuclein, inhibited ... 1B shows the pattern seen after the development of the blot. With increase in time of incubation, the intensity of the band for the mono...

Ngày tải lên: 14/02/2014, 19:20

... (83%) or LAC5 (83%). All together, recombination induced swapping of amino acids for 94 positions (19% of the residues) in the original LAC3 sequence. The C- and N-termini of the hybrids were 33–37% ... chimera, recombination preserved the integrity of domain D1 and the very end part of domain D3, two regions that interact precisely in the natural laccase fold (F...

Ngày tải lên: 18/02/2014, 06:20

... specifically bound to the induction of apoptosis, in agreement with recent findings [8]. Second, the intensity of the ML signal, correspond- ing to TG levels, is increased in irradiated MCF-7 cells, ... deconvolution of spectra of the type shown in Fig. 1A¢, with ML signals of a very low intensity, was performed as a first step. Deconvolution of spectra of the t...

Ngày tải lên: 18/02/2014, 13:20

... giving to the control samples a value ¼ 1 after deducting basal and background values. Sepsis resulted in a significant increase in the transcription of the mRNA encoding iNOS in heart of iNOS + ... ni- trosation, of mitochondrial components indirectly [35]. The free radical pathways of ONOO – are mainly initi- ated secondary to the reaction of ONOO – with CO 2 ,...

Ngày tải lên: 19/02/2014, 00:20

... efficient Hfq binding in vivo or that Hfq interacts with the 5¢rpsO RNA fragment which contains the translational operator of the rpsO messenger [37]. The phosphate number at the 5¢ end of the RNA does ... enzymes. The Hfq containing DNA fragment was excised with the same enzymes and ligated into the corresponding sites of pET11c and then transformed into the E....

Ngày tải lên: 19/02/2014, 16:20