Báo cáo khoa học: Pressure and heat inactivation of recombinant human acetylcholinesterase Importance of residue E202 for enzyme stability pdf

... inactivation of recombinant human acetylcholinesterase Importance of residue E202 for enzyme stability Ce ´ cile Cle ´ ry-Barraud 1 , Arie Ordentlich 2 , Haim Grosfeld 2 , Avigdor Shafferman 2 and Patrick ... conclusion, pressure and thermal stability of mutants provided evidence that the residue E202 is a determinant of structural and functional sta...

Ngày tải lên: 08/03/2014, 10:20

... measures of pitch maximum, min- imum, and range failed to capture even the basic contrast of rising versus falling contour, we extended our feature set with measures of slope of rise and slope of ... difficulty of identifying the onset of consonants of these classes. Pause-based features include number of pauses, average pause duration, total pause duration, and...

Ngày tải lên: 17/03/2014, 07:20

... gastric antrum. Examples of the second group are adipocytes and muscle cells (for a review, see [1]). Because of these interorgan differences in distribution and cellular concentration of GS, and because ... degree of activation of the GS promoter and that these interactions differ quantitatively between cells from hepatic and muscular origin. Materials and methods Sequ...

Ngày tải lên: 23/03/2014, 20:22

... metho- trexate. All of these and cGMP block low affinity [ 3 H]DNP- SG transport in human erythrocytes and so the low affinity transport of DNP-SG and the transport of cGMP may be mediated by one and the same ... inhibited all of the cGMP uptake with an estimated IC 50 value of 315 ± 70 l M .Theratioof this value to the apparent K d value for cGMP, about 5, is Fig. 5. Immu...

Ngày tải lên: 23/03/2014, 21:20

... Phoneme Conversion for Korean TTS Byeongchang Kim and WonI1 Lee and Geunbae Lee and Jong-Hyeok Lee Department of Computer Science & Engineering Pohang University of Science & Technology ... checking of the adjacent two phonetic morphemes. In the experiments with a corpus of 4,973 sentences, we achieved 99.9% of the grapheme- to-phoneme conversion performance...

Ngày tải lên: 20/02/2014, 18:20

... our set of output candidates to be complete. The set of output candidates is presented to the lexical search process, whose task it is to choose the most appro- priate candidate. For the example ... of output candidates for the example above contains 5 possibilities. The main task of the lexi- cal search process is to choose the most optimal candidate, thus the most natural-s...

Ngày tải lên: 17/03/2014, 06:20

... present evidence for existence of two distinct HSF1 isoforms in rainbow trout and the formation of heterotrimers of these isoforms in vitro. Materials and methods Cell culture and animals Rainbow ... characterization of two distinct isoforms of rainbow trout heat shock factor 1 Evidence for heterotrimer formation Nobuhiko Ojima and Michiaki Yamashita Cell Biology Secti...

Ngày tải lên: 19/02/2014, 12:20

... of deamida- tion of asparagine 146 on functional and structural properties of human lens aB-crystallin. Invest Ophthal- mol Vis Sci 45, 206–214. Small heat shock proteins and disease Y. Sun and ... (1999) Dementia, gliosis and expression of the small heat Y. Sun and T. H. MacRae Small heat shock proteins and disease FEBS Journal 272 (2005) 2613–2627 ª 2005 FEBS 262...

Ngày tải lên: 19/02/2014, 18:20

... oligomer mass and the proposed importance Fig. 8. Structural model of a p26 tetramer. (A) Sequence alignment of amino acid residues 59–158 of Artemia p26 (AAB87967) and residues 45–151 of wheat, Triticum ... model of p26 was constructed on the basis of the crystallin structure of wheat Hsp16.9 [14], a mono- disperse sHSP used for modeling of human aA- and aB-crysta...

Ngày tải lên: 07/03/2014, 12:20

... release of pressure. Enzymic activities of CPY, which was exposed to 400 MPa pressure at 10 °Cfor60min(s), at 0 °Cfor90min(d), and at )10 °Cfor 30 min (h), were measured. Table 3. Effect of urea and ... 2002 Cold inactivation of CPY under high pressure (Eur. J. Biochem. 269) 4669 Effect of glycerol and sucrose upon cold inactivation of CPY under high pressure CPY...

Ngày tải lên: 08/03/2014, 10:20