Báo cáo khoa học: X-ray crystallography, CD and kinetic studies revealed the essence of the abnormal behaviors of the cytochrome b5 Phe35fiTyr mutant pdf

... chain conformation of this residue. The shift of the Ca atom of Tyr35 of the Phe35fiTyr mutant from that of Phe35 of the wild-type cyt b 5 is 0.21 A ˚ , within the error limit. The side chain of Tyr35 of the Phe35fiTyr ... other mutants. However, the conformation of the latter is flexible. CD spectra of thermal denaturation of cyt b 5 and its Ph...

Ngày tải lên: 08/03/2014, 10:20

... from low amounts of pantoate [9]. Here, we report the results of the structural studies of the panto- ate-bound form of the N-terminal domain of E. coli PS (nPS). The structure and substrate-binding ... molecule takes the position of the adenine ring of ATP. The carbon and oxygen atoms of pantoate are shown in yellow and red, respectively. The carbon...

Ngày tải lên: 16/02/2014, 09:20

... Crystallization of the enzyme in the presence of the transition state analogue AlF 3 , the substrate Rib5P and the product AMP resulted in a structure with a bend arrangement of AMP-AlF 3 -AMP and with Rib5P ... interaction of hydrophobic amino acids of b-strands located on either side of the flexible catalytic loop. The tightly packed interface prevents the clo...

Ngày tải lên: 23/03/2014, 15:20

... surfaces of the protein. Com- parison of the structure of XAIP with that of xylanase inhibitor protein-I, and docking studies, suggest that loops a3–b4 and a4 b5 may be involved in the binding of GH11 ... from the protein surface into the solvent. There is yet another interesting feature of the XAIP structure which is related to the conformation of loop b3–a3...

Ngày tải lên: 29/03/2014, 09:20

... the coordinated loop dimension (EV1) and the His156-out dimension (EV2). The tra- jectory reaches the EV1 value of the open sulfate_1* structure (maximum 1.73) and exceeds the EV2 values of the ... carboxylate oxygen of the substrate bridges the magnesium ions at the active site of enolase: struc- ture of the yeast enzyme complexed with the equilibrium mi...

Ngày tải lên: 23/03/2014, 07:20

... Many of these stud- ies rely heavily on inhibitors that may affect the newly identified and structurally similar CPT1c. Therefore, some of these studies need to be re-evaluated in light of the ... ghrelin and adiponectin alter hypothalamic AMPK and malo- nyl-CoA levels [13,23,28,30–33]. The genetic evidence for the role of AMPK in the hypothalamus is less clear becaus...

Ngày tải lên: 14/02/2014, 22:20

... 158(21) and 296(154) [14–17]. The high specific activity of these FVIIa variants is presumably linked to a more stable burial of the N-terminus of the protease domain in the activation pocket of the ... included in the pub- lished alanine scanning mutagenesis study of FVIIa [13], and investigated the effects of the mutation on the enzymatic maturation of FVI...

Ngày tải lên: 18/02/2014, 08:20

... presenting the pulse of Src activity in the plane of the inactive and active fractions, s i and s 1 . If the duration of the stimulus exceeds the critical value, the trajectory in the (s i , ... conformation, the SH2 domain binds to pY i on the C-terminal tail, and the Src homology 3 (SH3) domain binds to the linker between the SH2 and kinase domains at...

Ngày tải lên: 18/02/2014, 11:20

... Sp1-binding sites, and all of these sites are required for full activation of the LDL receptor pro- moter. Similarly, a number of promoters of the SREBP target genes contain the SREs and certain proximal ... that the nuclear import occurs in the absence of importin-a. Furthermore, the dimerization of SREBPs via the leucine zipper domain is required for the inte...

Ngày tải lên: 18/02/2014, 13:20

... resulted in 55% and 51% loss of the initial activities of BHLmL2 and BHAb, respectively. These results strongly suggest that Ser is involved in the catalytic activity of BHLmL2 and BHAb and are consistent ... 97% inhibition of BHLmL2 and BHAb, respectively. The influence of pH on the activities of the BH enzymes was investigated in the pH range 6–11. The pH...

Ngày tải lên: 18/02/2014, 14:20