Báo cáo khoa học: Inactivation of annexin II tetramer by S-nitrosoglutathione pot
Báo cáo khoa học: Inactivation of annexin II tetramer by S-nitrosoglutathione pot
... changes in annexin II
[48] and may alter the environment of reactive sites of
annexin II by GSNO. We tested whether such changes
influence the GSNO inhibition of the activity of
annexin II. AIIt (5 ... the
activities of annexin II tetramer (AIIt), a member of the
Ca
2+
- dependent phospholipid-binding protein family.
Incubation of purified AIIt with S-nitrosogl...
Báo cáo khoa học: Inactivation of tyrosine phenol-lyase by Pictet–Spengler reaction and alleviation by T15A mutation on intertwined N-terminal arm docx
... C. freundii
TPL, consequently the l-DOPA productivity of T15A
was at least two-fold higher than that with C. freundii
Fig. 5. Inactivation of TPL enzymes by L-DOPA and its reactivation
by PLP. ... N-terminal
arm of C. freundii TPL. Biochemical characterization
of the native and mutant enzyme proved the mutation
on the interface increased the stability of the catalytic
capabili...
Tài liệu Báo cáo khóa học: Inactivation of copper-containing amine oxidases by turnover products doc
... cuvette closed by
a Teflon stopper. The oxygen was consumed by most
substrates within the mixing time as revealed by the
immediate bleaching of the TPQ 500 nm band and by the
appearance of absorption ... course of LCAO inactivation by spermidine. LCAO,
2.8 l
M
, was inactivated at 37 °Cby2.0m
M
substrate in 0.1
M
potas-
sium phosphate buffer at different pH values (open symbols...
Báo cáo khoa học: Regulation of arginase II by interferon regulatory factor 3 and the involvement of polyamines in the antiviral response potx
... percentage of cells
that were annexin V positive (annexin V
+
⁄ PI
–
and annexin V
+
⁄ PI
+
)
by flow cytometry. Plots in the lower panel illustrate the 8 h time
point. Data are representative of two ... following inducible expression of the consti-
tutively active form of IRF-3, IRF-3 5D [14]. Up-regu-
lation of ArgII gene expression was observed after
treatment of the tetr...
Báo cáo khoa học: Distribution of the extrinsic proteins as a potential marker for the evolution of photosynthetic oxygen-evolving photosystem II ppt
... Department of Biology, Faculty of Science, Tokyo University of Science, Tokyo, Japan
2 Institute of Biological Science, University of Tsukuba, Japan
3 Department of Biology, Faculty of Science, ... University
of Hyogo, for the generous supply of cells of C. parad-
oxa, C. gracilis and P. tricornutum. The present work
was supported in part by Grants-in-Aid for Scientific
R...
Báo cáo khoa học: Inactivation of phosphorylase is a major component of the mechanism by which insulin stimulates hepatic glycogen synthesis doc
... 2003
Stimulation of glycogen synthesis but not activation
of glycogen synthase by insulin can be explained
by inactivation of phosphorylase
To determine the role of inactivation of phosphorylase ... activation of hepatic glycogen synthase by insulin involving either inactivation of GSK-3
or inactivation of phosphorylase. Insulin phosphorylates and inactivates (–)...
Tài liệu Báo cáo khoa học: Modulation of a-synuclein aggregation by dopamine in the presence of MPTP and its metabolite pptx
... solu-
tion of ThT and the fluorescence intensity of the solu-
tion was measured at 482 nm. Figure 4A shows the
kinetics of aggregation of a-synuclein in the presence
of 100 lm MPTP and the effect of 50 ... shown to inhibit fibrillation of
a-synuclein by promoting the formation of nonamyloidogenic oligomers.
Fibrillation of a-synuclein is accelerated in the presence of pest...
Tài liệu Báo cáo khoa học: Regulation of cathepsin B activity by 2A2 monoclonal antibody docx
... the
presence of various concentrations of 2A2 mAb. The
binding of 2A2 mAb was followed by native gel elec-
trophoresis. Increasing the concentration of 2A2 mAb
at a fixed molar ratio of cathepsin ... &
Bjork I (2000) Cystatin inhibition of cathepsin B
requires dislocation of the proteinase occluding loop.
Demonstration by release of loop anchoring through
mutation of...
Tài liệu Báo cáo khoa học: Phosphorylation of hormone-sensitive lipase by protein kinase A in vitro promotes an increase in its hydrophobic surface area ppt
... incubated for a period of 30 min at
37 °C before the reaction was quenched by the addition of
3.25 mL of methanol ⁄ chloroform ⁄ heptane (10 : 9 : 7) and
1.1 mL of 0.1 m potassium carbonate and ... hydrolysis of triolein by purified
recombinant rat adipocyte HSL, with a decrease in the effect of bis-ANS
upon PKA phosphorylation of HSL. The interaction of HSL with bis-ANS
wa...
Tài liệu Báo cáo khoa học: Attenuation of cardiac mitochondrial dysfunction by melatonin in septic mice ppt
... com-
plex II were significantly lower, and those of com-
plex III and IV were significantly higher, in iNOS
– ⁄ –
Fig. 2. Effects of aMT treatment on CLP-induced mRNA levels
of iNOS. Ten nanograms of ... regarding ATP production suggest
that the low activities of complex I and II in iNOS
– ⁄ –
mice are compensated by the higher activities of com-
plex III and IV, allowing norm...