Báo cáo khoa học: NMR investigations of subunit c of the ATP synthase from Propionigenium modestum in chloroform/methanol/water (4 : 4 : 1) pot

... is due to the interaction of the monomer with the artificial environment lacking the stabilizing protein/protein contacts within the ring. The undecameric c ring of the ATP synthase from Ilyobacter ... ch loroform/meth anol/water (4 : 4 : 1) [23], amino-acid sequence of P. modestum subunit c with the helix locations in (b) SDS micelles [ 24...

Ngày tải lên: 08/03/2014, 10:20

... obtained from 3D experiments correlating the amide NH with the C signal of the preceding amino acid, correlating the amide HN and the Ca signals, correlating the amide HN and the Ca signal of the ... detectable. Effect of mutations in the minidomain on stop codon specificity Superposition of the NMR structure of the human C- domain on the full-length c...

Ngày tải lên: 06/03/2014, 11:20

... ey characteristic of the structure of the native epitope in Me 2 SO is the presence of a large number of electrostatic interactions, especially in the central region of the peptide. The side chain ... I-A u because o f occupation of the P1 pocket. Recent thermodynamic a nd kinetic studies of the b inding of TCRs to peptide–MHC ligands suggested that the l...

Ngày tải lên: 07/03/2014, 16:20

... anti-IFN -c Ig, of LPS-induced NO production in murine PEC but not in the RAW2 64. 7 murine macrophage cell line Cells of murine PEC and of the RAW2 64. 7 murine macrophage cell line were cultured ... of cofactors such as cytokines. In the case of region 2 activation, stimulation of the macrophages with interferon -c (IFN -c) , but not with LPS, was found to be eff...

Ngày tải lên: 17/03/2014, 10:20

... finally examined the effect of deletion of the components of the PDZ binding motif on the signal- ing properties of the receptor in Ca 2+ imaging experiments. Truncation of the receptor from the final ... of PDZ binding motifs, occurring at the C- terminus of the interacting proteins [16]. We scanned the human olfactory receptor repertoire for putative bin...

Ngày tải lên: 18/02/2014, 13:20

... and [1- 14 C] acetyl- CoA (47 mCiÆmmol )1 ) were purchased from Moravek Biochemicals (Brea, CA, USA). 4- Coumaroyl-CoA, cinnamoyl-CoA, 4- methoxycinnamoyl-CoA, benzoyl-CoA, phenylacetyl-CoA and N-methylanthraniloyl-CoA ... substrate- binding pocket (the so-called ‘coumaroyl-binding pocket’ [3]) at the active site of the enzyme, and guides the course of the Claisen-type...

Ngày tải lên: 07/03/2014, 10:20

... ~:ntcnc¢ constilucnt obtained during the con~ of parsing is ulkcn as the Rsuh first-k rule: the sontcnc¢ constltmmt with ~hc highest probability among the first k c. o~s~ct¢d ¢rammadcal scnunac~ ... Construction principles: random mincit)le: at 1my ~ nmd~ly select It c~ adidatc conslJt ucnt to be constttlct~ probability selection l~rinciole: at any dmc the candi~llt¢ con...

Ngày tải lên: 08/03/2014, 07:20

... aggregation of which was induced by adding heparin. The aggregation kinetics was then derived from the time dependence of fluorescence intensity. As shown in Fig. 2A, the fluorescence intensity of R3 reached ... flexible to change their structures, depending on the hydrophobic and hydrophilic balance of the solvent. On the other hand, the calculation from the CD ell...

Ngày tải lên: 23/03/2014, 12:20

... studied activities of VpU is the induc- tion of CD4 degradation in the endoplasmatic reticu- lum of infected CD4 + cells. Only the cytoplasmic domain of VpU, together with any membrane anchor, is ... concentration of DPC (1.5 mm in H 2 O). The initial addition of 5 mm DPC caused the most pro- nounced change in the CD spectrum, whereas increasing the detergent c...

Ngày tải lên: 18/02/2014, 06:20

... the amide vector dynamics of this residue indicate local motions occurring essentially over the nanosecond timescale. The lack of fast internal motions reflects the crucial role of Gln50, which ... Leu26, the red color indicates the coupling of low-frequency and high-frequency dynam- ics. With the exception of His52, all the mentioned residues are located in the h...

Ngày tải lên: 18/02/2014, 16:20