Báo cáo khoa học: Characterization of isocitrate dehydrogenase from the green sulfur bacterium Chlorobium limicola A carbon dioxide-fixing enzyme in the reductive tricarboxylic acid cycle ppt

... 5¢ -A AAAA CATATGGCAAGCA AATCGACCATCATCTACAC-3¢, and antisense, 5¢-AAA AA GGATCCCGGCTGAAAACCGGGCTGCATTA-3¢) were designed for amplification of idh flanked with NdeI and BamHI sites (underlined). After ... Characterization of isocitrate dehydrogenase from the green sulfur bacterium Chlorobium limicola A carbon dioxide-fixing enzyme in the reductive tricarbo...

Ngày tải lên: 08/03/2014, 10:20

... lipids in cells from human adenocarcinomas Anna Maria Luciani 1 , Sveva Grande 1 , Alessandra Palma 1 , Antonella Rosi 1 , Claudio Giovannini 2 , Orazio Sapora 3 , Vincenza Viti 1 and Laura Guidoni 1 1 ... p.p.m., attributed to arachidonic acid chains, and at 0.93–2.04 p.p.m., attributed to linolenic acid chains on the basis of a comparison with lipid extracts and from the d...

Ngày tải lên: 18/02/2014, 13:20

... on similarities in amino acid sequences, groups together chitinases and proteins devoid of catalytic activity due to the substitution of a critical amino acid in the cata- lytic centre. This latter ... (5¢-CTTCCTCCGCTTCCATGA-3¢) and QaCgClp1 (5¢-CCATGAAGTCCGCGAATC-3¢); and QsCgClp2 (5¢-GCATAGCGATGTGGACGA-3¢) and QaCgClp2 (5¢-GAGGACCGAGACCGTGAA-3¢). The abbreviations ‘Qs’ a...

Ngày tải lên: 19/02/2014, 00:20

... vertebrate DNASE1 expression in vivo or in vitro, including characterization of the promoter region of the gene and the associated transcriptional factors. Therefore, delineation of the transcriptional regulation ... determine the relative abundance by comparing the copy number of transcripts containing exon 1a with the number starting from exon 1. The abun- d...

Ngày tải lên: 19/02/2014, 06:20

... primers: gatggatcccatATGGGTG TTGAAGTTGTA annealing around the start codon of the Ppi1 ORF and gactcgagATTAGTCGACTTCTTACGC annealing just before the putative transmembrane domain (capital letter in the ... Characterization of the interaction between the plasma membrane H + -ATPase of Arabidopsis thaliana and a novel interactor (PPI1) Corrado Viotti, Laura Luoni, Piero M...

Ngày tải lên: 19/02/2014, 07:20

... domain in blue and the C-terminal domains in violet. The assumed start of the second C-terminal domain is indicated with a black arrow. A. N. Larsen et al. Characterization of a Serratia proteinase ... the enzymatic activities originating from these strains (unpublished data). One of the marine bacteria showing peptidase activ- ity was closely related to Serratia...

Ngày tải lên: 19/02/2014, 07:20

... 5¢-TAT GGATCCTTAATTAGAAACAAACTGTCTGA TAAAC P2OH 5¢- CTCGAGATTAGAAACAAACTGTCTGATAAACC P1Z 5¢-ATA CCATGGCTGGAGAAGACTTTAAGATC P1ZH 5¢- CATATGACTGGAGACTTTAAGATC P2Z 5¢-TAT GGATCCTCACCACCCAATTTCGGAAAG P2ZH 5¢- CTCGAGCCACCCAATTTCGGAAAGT Table ... and His-tag, respectively. Underlined are the restriction enzyme sites. Primer Sequence P1O 5¢-ATA CCATGGACAAAACCCACAGTACAATG P1OH 5¢- CATATGCACAAAACC...

Ngày tải lên: 19/02/2014, 12:20

... Grazia U, Felli MP, Vacca A, Farina AR, Maroder M, Cappabianca L, Meco D, Farina M, Screpanti I, Frati L et al. (1994) Positive and negative regulation of the com- posite octamer motif of the interleukin ... (Primer Kin188; 5¢-dGAGA GGTACCGAATTAATCACAAGCAA ATCTTCTC-3¢, corresponding to NTs )119 to )94). (7) Prm3aab; pGL3b:Prm3aab & pGL3e:Prm3aab. (Primer Kin160; 5¢-dGAGA GGTACCGC...

Ngày tải lên: 19/02/2014, 16:20

... by a large vacuole containing anthocyanins. On the other hand, carnation petals have already provided a suit- able material for studying alterations of membrane structure and activity associated ... evolutionary pressures acting in the plant and the animal kingdoms. The liver carrier has presumably evolved to facilitate the uptake of the low concentrations of anthocyan...

Ngày tải lên: 20/02/2014, 01:20

... differences in binding of ICAM-4 and the other two ICAMs for the I domains. Many of these mAbs displayed similar inhibitory profiles in assays investigating the effects of the mAbs on the interaction of ... to localize the ligand binding sites in the leukocyte b 2 integrins. The a chains of the CD11/CD18 integrins contain an inserted approxi- mately 200-amin...

Ngày tải lên: 20/02/2014, 11:20