Báo cáo khoa học: Characterization of isocitrate dehydrogenase from the green sulfur bacterium Chlorobium limicola A carbon dioxide-ﬁxing enzyme in the reductive tricarboxylic acid cycle ppt

... 5¢ -A AAAACATATGGCAAGCAAATCGACCATCATCTACAC-3¢, and antisense, 5¢-AAAAAGGATCCCGGCTGAAAACCGGGCTGCATTA-3¢)were designed for ampliﬁcation of idh ﬂanked with NdeIand BamHI sites (underlined). After ... Characterization of isocitrate dehydrogenase from the green sulfur bacterium Chlorobium limicola A carbon dioxide-ﬁxing enzyme in the reductive tricarboxylic acid cycle Tadayoshi Kanao, Mineko ... by DNA Data Bank of Japan(DDBJ). The sequence data was analyzed usingGENETYXsoftware package (Software Development, Tokyo, Japan). The nucleotide sequence data of Cl-idh will appear in the EMBL,...

Tài liệu Báo cáo khoa học: Characterization of 1H NMR detectable mobile lipids in cells from human adenocarcinomas doc

... lipids in cells from human adenocarcinomasAnna Maria Luciani1, Sveva Grande1, Alessandra Palma1, Antonella Rosi1, Claudio Giovannini2,Orazio Sapora3, Vincenza Viti1and Laura Guidoni11 ... p.p.m., attributed to arachidonic acid chains, andat 0.93–2.04 p.p.m., attributed to linolenic acid chainson the basis of a comparison with lipid extracts and from the data available in the literature, ... FEBS(Fig. 1A) , the corresponding 2D COSY spectra werealso characterized by prominent cross peaks from fatty acid chains (Fig. 1B), in agreement with the data avail-able in the literature [15] and...

Tài liệu Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas docx

... onsimilarities in amino acid sequences, groups togetherchitinases and proteins devoid of catalytic activity dueto the substitution of a critical amino acid in the cata-lytic centre. This latter ... (5¢-CTTCCTCCGCTTCCATGA-3¢)and QaCgClp1 (5¢-CCATGAAGTCCGCGAATC-3¢); andQsCgClp2 (5¢-GCATAGCGATGTGGACGA-3¢) andQaCgClp2 (5¢-GAGGACCGAGACCGTGAA-3¢). The abbreviations ‘Qs’ and ‘Qa’ refer, respectively, ... design wasbased on the conserved amino acid sequences of the catalytic domain of members of the GH18 familyresulted in the ampliﬁcation of an expected 147 bpsequence. Cloning and sequencing of...

Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt

... vertebrate DNASE1 expression in vivo or in vitro, including characterization of the promoterregion of the gene and the associated transcriptionalfactors. Therefore, delineation of the transcriptionalregulation ... determine the relative abundance by comparing the copy number of transcripts containing exon 1a with the number starting from exon 1. The abun-dance of transcripts starting from exon 1 was 10-foldhigher ... 10-foldhigher than that of the transcripts starting from exon 1a in pancreas, whereas it was half in QGP-1cells.To examine whether transcription starting from both exon 1a and exon 1 results in the production...

Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc

... primers: gatggatcccatATGGGTGTTGAAGTTGTA annealing around the start codon of the Ppi1 ORF and gactcgagATTAGTCGACTTCTTACGCannealing just before the putative transmembrane domain(capital letter in the ... Characterization of the interaction between the plasmamembrane H+-ATPase of Arabidopsis thaliana and a novelinteractor (PPI1)Corrado Viotti, Laura Luoni, Piero Morandini and Maria Ida ... thaliana (AHA1) interacts with the ﬁrst 88 aminoacids of PPI1 [22], indicating that the PM H+-ATPasebinding site of PPI1 is localized therein. Indeed, fusionproteins containing the ﬁrst 88 amino...

Tài liệu Báo cáo khoa học: Characterization of a recombinantly expressed proteinase K-like enzyme from a psychrotrophic Serratia sp. ppt

... domain in blueand the C-terminal domains in violet. The assumed start of the second C-terminaldomain is indicated with a black arrow. A. N. Larsen et al. Characterization of a Serratia proteinase ... the enzymatic activitiesoriginating from these strains (unpublished data).One of the marine bacteria showing peptidase activ-ity was closely related to Serratia proteamaculans of the Serratia ... 176–182.36 Narinx E, Baise E & Gerday C (1997) Subtilisin from psychrophilic antarctic bacteria: characterization and A. N. Larsen et al. Characterization of a Serratia proteinase K-like enzyme FEBS...

Tài liệu Báo cáo khóa học: Characterization of the products of the genes SNO1 and SNZ1 involved in pyridoxine synthesis in Saccharomyces cerevisiae pptx

... 5¢-TATGGATCCTTAATTAGAAACAAACTGTCTGATAAACP2OH 5¢-CTCGAGATTAGAAACAAACTGTCTGATAAACCP1Z 5¢-ATACCATGGCTGGAGAAGACTTTAAGATCP1ZH 5¢-CATATGACTGGAGACTTTAAGATCP2Z 5¢-TATGGATCCTCACCACCCAATTTCGGAAAGP2ZH 5¢-CTCGAGCCACCCAATTTCGGAAAGTTable ... and His-tag, respectively.Underlined are the restriction enzyme sites.Primer SequenceP1O 5¢-ATACCATGGACAAAACCCACAGTACAATGP1OH 5¢-CATATGCACAAAACCCACAGTACP2O 5¢-TATGGATCCTTAATTAGAAACAAACTGTCTGATAAACP2OH ... the absorbance of the supernatant was read at 363 nm. The absorbance of the reduced form of APAD (APADH) was linear over the 2–100 lMrange of glutamate with a molar extinctioncoefﬁcient of...

Tài liệu Báo cáo khoa học: Characterization of promoter 3 of the human thromboxane A2 receptor gene A functional AP-1 and octamer motif are required for basal promoter activity docx

... Grazia U, Felli MP, Vacca A, Farina AR, Maroder M,Cappabianca L, Meco D, Farina M, Screpanti I, Frati Let al. (1994) Positive and negative regulation of the com-posite octamer motif of the interleukin ... (PrimerKin188; 5¢-dGAGAGGTACCGAATTAATCACAAGCAAATCTTCTC-3¢, corresponding to NTs )119 to )94).(7) Prm3aab; pGL3b:Prm3aab & pGL3e:Prm3aab. (PrimerKin160; 5¢-dGAGAGGTACCGCAAATCTTCTCTCGCCTCC-3¢, ... Aiba Y, Nakamura K, Namba T, HirataM, Mazda O, Katsura Y & Narumiya S (1993)Thromboxane A2 receptor is highly expressed in mouseimmature thymocytes and mediates DNA fragmentationand apoptosis....

Tài liệu Báo cáo khoa học: Characterization of electrogenic bromosulfophthalein transport in carnation petal microsomes and its inhibition by antibodies against bilitranslocase docx

... by a large vacuole containing anthocyanins. On the otherhand, carnation petals have already provided a suit-able material for studying alterations of membranestructure and activity associated ... evolutionary pressures acting in the plant and the animal kingdoms. The liver carrierhas presumably evolved to facilitate the uptake of the low concentrations of anthocyanins found in plasmaafter ingestion ... Àðk A =k0Þ= A ð1Þwhere k A and k0are the inactivation rate constantseither in the presence or in the absence of variousconcentrations of a ligand A, k2and k1are the rate constants of...

Tài liệu Báo cáo khoa học: Characterization of ICAM-4 binding to the I domains of the CD11a/CD18 and CD11b/CD18 leukocyte integrins pptx

... differences in binding of ICAM-4 and the other two ICAMs for the I domains.Many of these mAbs displayed similar inhibitory proﬁles in assays investigating the effects of the mAbs on the interaction of ... to localize the ligandbinding sites in the leukocyte b2integrins. The a chains of the CD11/CD18 integrins contain an inserted approxi-mately 200-amino -acid intervening domain (I domain),which ... plasmid containing the cDNA fragment of the CD11b I domain was a generousgift from A. Arnaout (Massachusetts General Hospital,Boston, MA) [20]. The cDNA fragment of CD1 1a I domaininserted into...

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