Báo cáo khoa học: The involvement of human ribonucleases H1 and H2 in the variation of response of cells to antisense phosphorothioate oligonucleotides pot

... al. (Eur. J. Biochem. 269) Ó FEBS 2002 The involvement of human ribonucleases H1 and H2 in the variation of response of cells to antisense phosphorothioate oligonucleotides Anneloor L. M. A. ten ... as a single band on t hese gels, as the ODN h ybridizes to the center of the input target RNA. The asterisk indicates the input target RNA; the...

Ngày tải lên: 08/03/2014, 10:20

... (http://www.ncbi.nih.gov/blast/Blast.cgi). Binding of A1AT to preprohepcidin, prohepcidin and mature hepcidin The cDNA of A1AT was cloned into pTRG with the restric- tion sites EcoRI and XhoI, and the coding cDNAs of prep- rohepcidin, ... secretion. To generate the mature 25-AA hepcidin peptide, there is a furin cleavage site in the C-terminal part of prohepcidin...

Ngày tải lên: 16/03/2014, 01:20

... ORF of human fad49. We first used the human genome data- base to predict the ORF region of human fad49 by splicing out the introns and combining the exons of the ORF of human fad49. To isolate human ... SH3 domain in the same protein, and that this intramolecu- lar interaction suppresses the lipid-binding activity of the PX domain in the restin...

Ngày tải lên: 16/03/2014, 04:20

... handed over to the Storage Handler which decides how to store the resource. Since the output of the tagger is a XML document it is stored as a XML Database. Finally the in- formation about the ... to perform PoS tagging and has permission to read the document to be tagged. The next step is to fetch the docu- ment from the Storage Handler as input to th...

Ngày tải lên: 31/03/2014, 20:20

... intoxication, diphtheria toxin is cleaved by furin into two fragments: the A chain corresponding to the catalytic domain (C domain); and the B chain corresponding to the translocation domain ... translocation domain (T domain) and the receptor-binding domain. The C and T domains remain covalently linked by a disulfide bond. Following binding to its cell-surface receptor...

Ngày tải lên: 16/02/2014, 09:20

... applied to clarify whether the stoichiometry of the c rings is in uenced by variation of the expression level of subunit c. In the present study we investigated whether the c subunits from the sodium ... from the bottom of the cylinder and forms the central stalk together with the e subunit. A striking feature of the structure is an inherent asymmetry...

Ngày tải lên: 07/03/2014, 21:20

... measure the binding interactions of small molecules to the ligand-binding domain (LBD) of human estrogen receptor [15]. To the best of our knowledge, there is to date no report concerning the ligand–PPAR ... between the SPR binding affinities and Auto- Dock-predicted binding free energies. Table 3. The binding free energies of the ligands binding to PPARc. The...

Ngày tải lên: 16/03/2014, 16:20

... tetracycline-resistance cassette into the HindIII site. pRS239 was obtained by inserting the tetra- cycline-resistance cassette into the HindIII site of pRS155, encoding glnK under the control of t ... a majority of NifL was sequestered to the cytoplasmic mem- brane. These findings indicate that inhibition of NifA in the presence of oxygen and/ or ammonium occurs via...

Ngày tải lên: 07/03/2014, 16:20

... but the larger b subunit, in addi- tion to possessing both the polymerase and RNase H domains found in the a subunit, also contains a C-ter- minal region corresponding to the viral integrase. The ... RNA strands in red and DNA strands in black. In the internal cleavage mode, the arrows mark the sites of cleavage along the length of the hybrid where nu...

Ngày tải lên: 30/03/2014, 02:20

... saline (NaCl ⁄ P i ) and chased for indicated times in DMEM containing an excess of cold methionine. To determine the effect of various pro- tease inhibitors, cells were treated with inhibitors ... degradation of the phosphoinositidase C-linked guanine-nucleotide-binding protein Gq alpha ⁄ G11 alpha following activation of the human M1 muscarinic acetylcholine receptor...

Ngày tải lên: 20/02/2014, 03:20