Báo cáo khoa học: N-Methylation in polylegionaminic acid is associated with the phase-variable epitope of Legionella pneumophila serogroup 1 lipopolysaccharide pptx

... mAb LPS -1 bound only to LPS species lacking the mAb 2625 epitope, thus suggesting that the mAb 2625 epitope inte rferes with the binding of mAb LPS -1. The OPS of L. pneumophila Sg 1 is a polylegionaminic acid ... and the loss of the mAb 2625 epitope is one of them. Phase variation in L. pneumophila was f ound to in uence other LPS biosynthesis p...

Ngày tải lên: 08/03/2014, 10:20

... to be able to interpret the way is which this is achieved, but the results show the complexity of the interactions that occur both within the mature protein and in the acquisition of its tertiary structure. In ... a C-terminal domain of either 16 or 10 0 amino acids [7]. The first two genes of t he array, CPB1 and CPB2, are atypical because they encode enzymes...

Ngày tải lên: 23/03/2014, 13:20

... distribution of the peak at m ⁄ z ¼ 9 01. 18 indicates a bromine-containing peptide (Fig. 1A,B, inset). The peak at m ⁄ z ¼ 626.29 is consis- tent with amidation of the C-terminal fragment (DVVCS), as is the ... exhibits a 14 Da mass increase consistent with methylation of the side chain carboxyl group of the N-terminal Asp residue confirm- ing amidation of the...

Ngày tải lên: 23/03/2014, 11:20

... constraints. One of the central global constraints in the field of corefer- ence resolution evolves from the interplay of intra- sentential binding constraints and the transitivity of the anaphoric ... preceding 1 to 0. The costs of swapping a preceding 1 to 0 are higher than the gain from swapping the 0 to 1 (as a consequence of the Balas ordering). So no...

Ngày tải lên: 31/03/2014, 20:20

... [20]. Extending this to mixtures of large proteins (see below), nozzle-skimmer dissociation spectra of 1 : 1, 2 : 1 and 3 : 1 mixtures of 14 4 and 11 6 kDa proteins showed the corresponding molecular ... after eliminating the mistakenly pre- dicted N-terminal Met. The fragment ions containing the C-terminus have the predicted mass values going back 10 residues to Cy...

Ngày tải lên: 18/02/2014, 16:20

... A (19 75) Pro- ceedings: Selective oxidation of methionine residues in proteins. Isr J Med Sci 11 , 11 71. 17 Matheson NR, Wong PS & Travis J (19 79) Enzymatic inactivation of human alpha -1- proteinase ... 3.0 ± 1. 0 · 10 )11 3.6 ± 0.5 · 10 6 1. 1 ± 0.2 · 10 )4 NCS 9.2 ± 2.7 · 10 )9 1. 0 ± 0.3 · 10 6 9.0 ± 3 .1 · 10 )3 MPO 5.8 ± 0.9 · 10 )9 ND ND Pr3 Elafin No...

Ngày tải lên: 19/02/2014, 07:20

... removal of the NDA amino acid region abolishes proper nuclear targeting of the mSMOl isoform. Results Structural analysis and modeling of vertebrate SMO proteins Comparison of the derived amino acid ... has not been shown to contain any residue involved in either the catalytic site or the FAD-binding domain [9 ,10 ,12 ,13 ]. Interest- ingly, this 31 amino acid regio...

Ngày tải lên: 07/03/2014, 17:20

... O-chain LPSs of wild-type RC1, from the LPS of a s pontaneous phase variant (strain 811 ), and an isogenic mutant ( strain 5 215 ) [15 ]. In the present study we investigated the binding of the antibody to the ... [5,9]. In L. pneumophila Sg 1 LPS the OPS is linked to a terminal nonreducing L -rhamnose (Rha II ) of the core oligosaccharide [10 ,11 ]. The cor...

Ngày tải lên: 08/03/2014, 10:20

... protein FEBS Journal 278 (2 011 ) 211 7– 213 0 ª 2 011 The Authors Journal compilation ª 2 011 FEBS 212 1 These results indicate that N63 interacts obviously with the precipitation of CaCO 3 because it induces drastic ... gel. Nautilin-63, a novel shell nacre protein B. Marie et al. 212 0 FEBS Journal 278 (2 011 ) 211 7– 213 0 ª 2 011 The Authors Journal compilation ª 2 011 FEBS do...

Ngày tải lên: 22/03/2014, 16:20

... susceptible. These findings show that the presence of Val67 is one of the essential factors responsible for the actin-binding capacity of these snake DNases I. Tissue distribution of snake DNases I The ... be involved in changing the thermally stable characteristics of vertebrate DNases I, substitution of Ile130Leu in snakes and insertion of Ser205 in amphibia....

Ngày tải lên: 20/02/2014, 23:20