Báo cáo khoa học: Antibacterial peptides in stimulated human granulocytes Characterization of ubiquitinated histone H1A doc

... Antibacterial peptides in stimulated human granulocytes Characterization of ubiquitinated histone H1A Yuqin Wang, William J. Grif®ths, Hans JoÈ rnvall, ... ubiquitination of histone H1Ausing electrospray ( ES) MS. The tripeptide A rg-Gly-Gly, corresponding to the three C-terminal residues of ubiquitin, was found to be linked to Lys222 of histone H1A. MATERIALS ... suggesting immunolog-ical roles of histones, in addition to the classical function of histones in chromatin conformation. In phytothaemagglu-tinin-activated human peripheral lymphocytes, histone...

Tài liệu Báo cáo khoa học: Catabolite repression in Escherichia coli – a comparison of modelling approaches docx

... composed of EIIA, B and C domains, with the EIIA and B do-mains being part of the phosphorylation chain and theEIIC domain representing the membrane domain. Asall components of the PTS, depending ... phosphory-lation of glucose and a number of other carbohydrates(Fig. 1). It consists of two common cytoplasmic pro-teins, enzyme I (EI) and histidine-containing protein(HPr), as well as an array of carbohydrate-speciﬁcenzyme ... system and, in this function, is one of the key players of cataboliterepression. Mathematical modelling of signal transduction and gene expres-sion of the enzymes involved in the transport of carbohydrates...

Tài liệu Báo cáo khoa học: Compartmentalization and in vivo insulin-induced translocation of the insulin-signaling inhibitor Grb14 in rat liver pptx

... The binding of ZIP to Grb14, byrecruiting protein kinase Cf, enhances the serine phos-phorylation of Grb14 and its inhibitory effect on IRkinase and insulin signaling [10]. The interaction of Grb14 ... tyrosine phosphorylation in both activation and inhibition of the insulin recep-tor tyrosine kinase in vivo. Endocrinology 137, 4960–4968.24 Kelly KL & Ruderman NB (1993) Insulin -stimulated phosphatidylinositol ... reg-ulator of liver insulin signaling, and that it may repre-sent an interesting molecule with which to regulateliver insulin action.Experimental proceduresMaterialsPorcine insulin, recombinant human...

Tài liệu Báo cáo khoa học: Proton transfer in the oxidative half-reaction of pentaerythritol tetranitrate reductase Structure of the reduced enzyme-progesterone complex and the roles of residues Tyr186, His181 and His184 pdf

... (gradient of 15–150 mm NaCl contained in 1 L buffer A) to obtain pure protein.Redox potentiometry, ligand binding andstopped-ﬂow kinetic analysesRedox titrations and the determination of redox ... 6-S-cysteinyl ﬂavin mononucleotide-containinghistamine dehydrogenase from Nocardioides simplex:molecular cloning, sequencing, overexpression, and characterization of redox centers of enzyme. ... systematicanalysis of the contribution of each histidine residue tobinding and catalysis. Thus, to ascertain the role of each histidine residue, and to identify any differentialcontribution to binding and...

Báo cáo khoa học: Tyrosine nitration in the human leucocyte antigen-Gbinding domain of the Ig-like transcript 2 protein ppt

... an increase in binding capacity[25].Identiﬁcation of nitration site in the extracellulardomain of ILT2 In the extracellular domain of ILT2, there are severalTyr residues that participate in ... resultsindicate that NO maintains, or even increases, ILT2-mediated suppression in NKL cells.After HLA-G binding, immunoreceptor tyrosine-based inhibitory motifs in the cytoplasmic tail of theILT2 ... with different concen-trations of SIN-1. As shown in Fig. 3, SIN-1treatment signiﬁcantly increased rhILT2-Fc binding toHLA-G (150 ± 18%; HLA-G binding to SIN-1 2 mmtreated rhILT2-Fc compared...

Báo cáo khoa học: Natriuretic peptides affect Pseudomonas aeruginosa and speciﬁcally modify lipopolysaccharide biosynthesis doc

... We investi-gated the possible involvement of Vfr in the action of natriuretic peptides on P. aeruginosa, using strainPAO9002, a vfr null mutant of P. aeruginosa PAO1obtained by insertion of ... intrabacterialconcentrations of cAMP and cGMP. The concentra-tion of cAMP in P. aeruginosa PAO1 grown in ordin-ary nutrient broth medium (BNO) in the absence of treatment was determined by ELISA. The valueobtained in ... machine was run in positive mode andcalibrated with 1 lL of a 10 pm protein standard mixture of angiotensin II, adrenocorticotrophic hormone Clip (18–39), human insulin, oxidized B-chain, bovine...

Báo cáo khoa học: "AMBIGUITY RESOLUTION IN THE HUMAN SYNTACTIC PARSER: AN EXPERIMENTAL STUDY" ppt

... to determining the functional orga- nization of the human parser. In Section l, a pre- liminary taxonomy of parsing models is presented, based upon a consideration of modes of handling ambiguities; ... so far. Instead of the parser, after making a single or parallel analysis of an ambiguity, maintaining the analysis/es as further input is received, one can imagine it just dropping whatever ... points of Chapter 3 of Kurtzman, 1984.) Models of the human syntactic parsing mecha- nism can be classified according to the ways in which they operate upon ambiguous input. Each mode of...

Tài liệu Báo cáo khoa học: Branched N-glycans regulate the biological functions of integrins and cadherins doc

... resulted in enhanced integrin clustering and activation of a5b1transcription by protein kinase C signaling, which, in turn, upregulated cell surface expression of a5b1,resulting in increased ... functionIntegrin sugar chains play important roles in thebiological functions of integrinsA growing body of evidence indicates that the presence of the appropriate oligosaccharide can modulate inte-grin ... in GnT-V-transfected cells [58]. Increased num-bers of branched sugar chains inhibited a5b1clustering and organization of F-actin into extendedmicroﬁlaments in cells plated on ﬁbronectin-coatedplates....

Tài liệu Báo cáo khoa học: Deciphering enzymes Genetic selection as a probe of structure and mechanism docx

... folding of an enzyme. I. Theory of protein engineering anaylsis of sta-bility and pathway of protein folding. J. Mol. Biol. 224, 771–782.21. Clackson, T. & Wells, J.A. (1995) A hot spot of ... of exacting structuralcriteria; only properly folded proteins are active. Proteinfolding is dictated by amino acid sequence [12]. In anensemble of proteins composed from the standard set of ... import-ance of certain interactions in the hydrophobic core [19].Phe77 may represent a Ôhot spotÕ for binding energy duringprotein folding [20], analogous to those found for receptor–ligand interactions...

Tài liệu Báo cáo khoa học: A systems biology approach for the analysis of carbohydrate dynamics during acclimation to low temperature in Arabidopsis thaliana doc

... successful metabolic engineering of freezing tolerance in plants should include such ananalysis of the dynamics of metabolism to gain com-prehensive information about the effects of individualoverexpression ... the integral of carbon uptake perhour. Data points were spline-interpolated to obtain time-continuous information on net photosynthesis during thewhole period of cold exposure. The rate of ... structure of C24. Doc. S1b. sbml format of model structure of C24. Doc. S2a. Model structure of Rsch. Doc. S2b. sbml format of model structure of Rsch. Doc. S3. Documentation of the model structure.This...

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