Báo cáo khoa học: Antibacterial peptides in stimulated human granulocytes Characterization of ubiquitinated histone H1A doc

... Antibacterial peptides in stimulated human granulocytes Characterization of ubiquitinated histone H1A Yuqin Wang, William J. Grif®ths, Hans JoÈ rnvall, ... ubiquitination of histone H1Ausing electrospray ( ES) MS. The tripeptide A rg- Gly-Gly, corresponding to the three C-terminal residues of ubiquitin, was found to be linked to Lys222 of histone H1A. MATERIA...

Ngày tải lên: 08/03/2014, 10:20

... composed of EIIA, B and C domains, with the EIIA and B do- mains being part of the phosphorylation chain and the EIIC domain representing the membrane domain. As all components of the PTS, depending ... phosphory- lation of glucose and a number of other carbohydrates (Fig. 1). It consists of two common cytoplasmic pro- teins, enzyme I (EI) and histidine-containing protein (HPr), a...

Ngày tải lên: 18/02/2014, 13:20

... The binding of ZIP to Grb14, by recruiting protein kinase Cf, enhances the serine phos- phorylation of Grb14 and its inhibitory effect on IR kinase and insulin signaling [10]. The interaction of Grb14 ... tyrosine phosphorylation in both activation and inhibition of the insulin recep- tor tyrosine kinase in vivo. Endocrinology 137, 4960– 4968. 24 Kelly KL & Ruderman NB (1993)...

Ngày tải lên: 18/02/2014, 18:20

... (gradient of 15–150 mm NaCl contained in 1 L buffer A) to obtain pure protein. Redox potentiometry, ligand binding and stopped-flow kinetic analyses Redox titrations and the determination of redox ... 6-S-cysteinyl flavin mononucleotide-containing histamine dehydrogenase from Nocardioides simplex: molecular cloning, sequencing, overexpression, and characterization of redox centers of...

Ngày tải lên: 20/02/2014, 02:21

... an increase in binding capacity [25]. Identification of nitration site in the extracellular domain of ILT2 In the extracellular domain of ILT2, there are several Tyr residues that participate in ... results indicate that NO maintains, or even increases, ILT2- mediated suppression in NKL cells. After HLA-G binding, immunoreceptor tyrosine- based inhibitory motifs in the cytopl...

Ngày tải lên: 07/03/2014, 02:20

... We investi- gated the possible involvement of Vfr in the action of natriuretic peptides on P. aeruginosa, using strain PAO9002, a vfr null mutant of P. aeruginosa PAO1 obtained by insertion of ... intrabacterial concentrations of cAMP and cGMP. The concentra- tion of cAMP in P. aeruginosa PAO1 grown in ordin- ary nutrient broth medium (BNO) in the absence of treatment...

Ngày tải lên: 07/03/2014, 05:20

... to determining the functional orga- nization of the human parser. In Section l, a pre- liminary taxonomy of parsing models is presented, based upon a consideration of modes of handling ambiguities; ... so far. Instead of the parser, after making a single or parallel analysis of an ambiguity, maintaining the analysis/es as further input is received, one can imagine it j...

Ngày tải lên: 08/03/2014, 18:20

... resulted in enhanced integrin clustering and activation of a 5 b 1 transcription by protein kinase C signaling, which, in turn, upregulated cell surface expression of a 5 b 1 , resulting in increased ... function Integrin sugar chains play important roles in the biological functions of integrins A growing body of evidence indicates that the presence of the appropriate oligos...

Ngày tải lên: 18/02/2014, 17:20

... folding of an enzyme. I. Theory of protein engineering anaylsis of sta- bility and pathway of protein folding. J. Mol. Biol. 224, 771–782. 21. Clackson, T. & Wells, J.A. (1995) A hot spot of ... of exacting structural criteria; only properly folded proteins are active. Protein folding is dictated by amino acid sequence [12]. In an ensemble of proteins composed from the sta...

Ngày tải lên: 19/02/2014, 12:20

... successful metabolic engineering of freezing tolerance in plants should include such an analysis of the dynamics of metabolism to gain com- prehensive information about the effects of individual overexpression ... the integral of carbon uptake per hour. Data points were spline-interpolated to obtain time- continuous information on net photosynthesis during the whole period of co...

Ngày tải lên: 14/02/2014, 22:20