... data.Data analysesData were analysed by means of sigmaplot 2001 (SPSSScience Software Gmbh, Erkrath, Germany). Data for the characterization of the kinetics of electrogenic BSP uptakefitted the Michaelis–Menten ... process.litre/osmol01234nmoles of BSP disappeared0,00,40,81,21,6Fig. 2. The dependence of valinomycin-induced disappearance of BSP on the osmolarity of the extra-vesicular medium in the pres-ence of carnation ... presence or in the absence of variousconcentrations of a ligand A, k2and k1are therate constants of the inhibition of the bilitranslocase–bilirubin complex and of free bilitranslocase, respect-ively....