Báo cáo khoa học: Characterization of glycosphingolipids fromSchistosoma mansoni eggs carrying Fuc(a1±3)GalNAc-, GalNAc(b1±4)[Fuc(a1±3)]GlcNAc-and Gal(b1±4)[Fuc(a1±3)]GlcNAc- (Lewis X) terminal structures pot

... Schistosoma mansoni egg glycolipids (Eur. J. Biochem. 269) 493 Characterization of glycosphingolipids from Schistosoma mansoni eggs carrying Fuc(a1±3)GalNAc-, GalNAc(b1±4)[Fuc(a1±3)]GlcNAc- and Gal(b1±4)[Fuc(a1±3)]GlcNAc- ... University of Giessen, Germany; 2 School of Biological Sciences, University of Wales, Bangor, Wales, UK The carbohydrate moieties of glycosphi...

Ngày tải lên: 08/03/2014, 10:20

... 10 6 mL )1 . Next, 0.5 mL of propidium iodide staining solution (10 mg of propidium iodide, 0.1 mL of Triton-X, 3.7 mg of EDTA in 100 mL of NaCl ⁄ P i ) was added to 0.5 mL of cell suspension. The ... were analysed in terms of production ⁄ consumption of TG. In mammalian cells, TG may be deleted to meet the needs of cell metabolism, either as a source of energy or as a res...

Ngày tải lên: 18/02/2014, 13:20

... addi- tional C -terminal sequence of about 90 amino acids of unknown function. The high degree of identity of the Cg-Clp1 and Cg-Clp2 Glyco_18 domains (84% iden- tity) argues for a conservation of the ... an additional 90 amino acid C -terminal sequence of unknown function (Fig. 1). Hence, the overall structure of Cg-Clp2 is similar to that of Cg-Clp1. Expression of Cg-Clp...

Ngày tải lên: 19/02/2014, 00:20

... followed by mixture with 6 lL of Lipofectamine Plus reagent at room temperature for 10 min. Four microliters of Lipofectamine Plus reagent were diluted in 100 lL of Opti-MEM I-reduced serum med- ium. ... Department of Legal Medicine and Medical Genetics, Gunma University, Japan 2 Division of Medical Genetics and Biochemistry, University of Fukui, Japan 3 Division of Legal Medici...

Ngày tải lên: 19/02/2014, 06:20

... the mechanism of action of PPI1. The analysis of the pH dependence of PPI1- induced activation of the H + -ATPase showed that stimulation decreases dramatically with the increase of pH above pH ... with PPI1 588 His 6 . The analysis of the effect of PPI1 588 His 6 on the dependence of PM H + -ATPase activity on the concen- tration of MgATP (Fig. 3) showed that stimulation...

Ngày tải lên: 19/02/2014, 07:20

... from Tritirachium album Limber. Characterization of the chromosomal gene and expression of the cDNA in Escherichia coli. Eur J Biochem 179, 185–194. Characterization of a Serratia proteinase K-like ... Willassen 1,2 1 Department of Molecular Biotechnology, Institute of Medical Biology, Faculty of Medicine, University of Tromsø, Norway 2 The Norwegian Structural Biology Centr...

Ngày tải lên: 19/02/2014, 07:20

... of all of these genes remains to be clarified. Expression and purification of Sno1p and Snz1p In light of the similarity in the amino acid sequence of Sno1p to that of the glutaminase subunit of ... with a library of yeast chromosomal DNA. It was found that 4.6 and 5.1 kb overlapping fragments of chromosome XIII carrying SNO1 and SNZ1 were capable of complementing the defe...

Ngày tải lên: 19/02/2014, 12:20

... differences between the TP isoforms in terms of their mechanisms of signaling [18,19], modes of regulation⁄ desensitiza- tion [20–23] and patterns of expression [24], this repre- sents a question of potentially ... or hypertrophic growth of VSM [5,6], inhibition of angio- genesis ⁄ neo-vascularization [7] and apoptosis of CD4 ⁄ CD8 ++⁄ – thymocytes [8]. Alterations in the leve...

Ngày tải lên: 19/02/2014, 16:20

... data. Data analyses Data were analysed by means of sigmaplot 2001 (SPSS Science Software Gmbh, Erkrath, Germany). Data for the characterization of the kinetics of electrogenic BSP uptake fitted the Michaelis–Menten ... process. litre/osmol 01234 nmoles of BSP disappeared 0,0 0,4 0,8 1,2 1,6 Fig. 2. The dependence of valinomycin-induced disappearance of BSP on the osmolarity of...

Ngày tải lên: 20/02/2014, 01:20

... of ICAM transfectants to 0.4 lg of I domain GST captured with 0.2 lg of coated anti-GST Ab. Controls include wells with captured GST and the binding of wild-type L cells. Background binding of ... Inhibition of adhesion of ICAM trans- fectants to purified CD11b I domain GST fusion protein. The effects of anti-ICAM and anti-I domain mAbs on adhesion of ICAM transfectants to 0.4...

Ngày tải lên: 20/02/2014, 11:20