Báo cáo Y học: Complexation of ytterbium to human transferrin and its uptake by K562 cells pot

... study of uptake of Yb 3+ by human Tf (hTf) by UV-vis and NMR spectroscopy, and inductively coupled plasma atomic emission spectro- metry (ICP-AES). The binding of Yb-bound Tf to human erythroleukemia ... filter. RESULTS Binding of Yb 3+ to apo-hTf The thermodynamics of binding of Yb 3+ to apoTf. Binding of Yb 3+ to apo-Tf was investigated by addition of...

Ngày tải lên: 08/03/2014, 09:20

... presence of acetylcholinesterase and butyrylcholinesterase mRNAs in kidney supports their synthesis in the organ itself, and the prevalence of glycosyl- phosphatidylinositol-anchored acetylcholinesterase ... origin of the butyrylcholinesterase activity assayed in kidney. This proposal is in agreement with the cytochemical staining of acetylcholinesterase in the capsule of Bowman...

Ngày tải lên: 06/03/2014, 22:21

... product of cellulolysis), and with a mixture of glucose and cellobiose. Cell extracts and extracellu- lar media were analysed by 2D NMR spectroscopy and by TLC to identify and quantify the oligosaccha- rides ... were separated by centrifugation and analysed, in parallel, by 1 H NMR spectroscopy and by TLC. Consumption of the substrates and pro- duction of t...

Ngày tải lên: 07/03/2014, 17:20

... proteins were resolved by 12.5% SDS/PAGE and analysed by Western blotting using an anti-RAS Ig (top). Proteins o f whole cell lysate were resolved by 12.5% SDS/PAGE and analysed by Western blotting ... be due to the a bility of TPO to activate the e arly events of the R AS signalling pathway. However, a recent study reported that inhibitors of PtdIns3K abolished potentia...

Ngày tải lên: 08/03/2014, 16:20

... by nickel-chelate chromatography to a specific activity of 1.06 UÆmg )1 protein at 2 5 °C and with a yield of 98%. The characterization of the purified enzyme showed that GO is a homotetramer of ... step by metal-chelate chromatography, producing a stable holoenzyme with a high specific activity and a 98% yield quickly and simply. Its properties (aggregation state, absorban...

Ngày tải lên: 08/03/2014, 16:20

... corresponding to the binding to A B Fig. 1. Spectroscopic analysis of ligand binding to C-LytA. (A) Con- formational changes induced by ligands on C-LytA monitored by near-UV CD with no ligand added (—) and ... order to establish the types of compound to be examined, and to reduce the num- ber of an otherwise vast set of candidates, we took into account: (a) commer...

Ngày tải lên: 19/02/2014, 05:20

... easily incorporated into the practice setting because of its brevity. A comparative study of MYMOP and the SF-36 has been conducted [1]. MYMOP concurrent validity was supported by its ability to ... words. Symptom 2 S2 Optional and is second symptom which is part of the same problem as symptom 1 Activity A An activity of daily living of importance to the patient in whi...

Ngày tải lên: 25/10/2012, 10:06

... delivery of a high concentration of active agents in the colon to improve pharmacotherapy and reduce its potential systemic toxicity and side effects [3]. The early detection, diagnosis, and ... esters of hydrogenated fatty acids. Hydro- genated cottonseed oil (Lubritab™ or Sterotex™), hydrogenated palm oil (Dynasan™ P60 or Softisan™ 154), hydrogenated castor oil (Cutina™...

Ngày tải lên: 25/10/2012, 11:22

... mechanism of action of all NSAIDs is the inhibition of the biosynthesis of prostaglandins by blocking the activity (selectively and/ or non-selectively) of two isoforms of cyclooxygenase enzyme ... aim of this work was to study the ability of β-cyclodextrin (β-CD) or hydroxypropyl β-cyclodextrin (HP-β-CD) to ameliorate the induction of gastric ulcers by a nonste...

Ngày tải lên: 26/10/2012, 08:57

... treatment [16]. 4. Indications of HBV Treatment Historically, levels of HBV DNA and ALT, and histological activity of liver biopsy have been used as the three main factors to determine if a patient ... On-treatment Monitoring and Duration of Therapy Patients who receive HBV treatment should be monitored regularly. The interval of follow-up visits may vary depending on r...

Ngày tải lên: 03/11/2012, 09:41