... with aF-N without competitors; and with competitors, lanes 4, 5 and 6, with C8; lanes 7, 8 and 9, with C5; lanes 10 , 11 and 12 , with C5Pc-2; lanes 13 , 14 and 15 , with C5gg; lanes 16 , 17 and 18 , ... extracts, 1 · 10 7NIH3T3 cells wereharvested and washed with NaCl/Pi, lysed with a 5 cell-pack vol. of lysis buffer consisting of 50 mMTris/HCl(pH 8.0), 14 0 mMNaCl, 1 mMEDTA, 1 mMEGTA,1mMdithiothreitol, ... 4 °C for 2 h and further incubated for 1 h with 50 lL of 50% slurry of protein A Sepharose. Afterwashing with the lysis buffer, immunoprecipitates wereeluted from protein A Sepharose by adding...