Báo cáo Y học: Characterization of a low redox potential laccase from the basidiomycete C30 pptx
Báo cáo Y học: Characterization of a low redox potential laccase from the basidiomycete C30 pptx
... and, although they share basic properties, the largevariation in catalytic activity for both phenolic and dyessupports this idea. The C30 laccase isoforms we have detected so far all havean apparent ... substrate level is low and a highcapacity /low specificity system when the substrate is abun-dant.In conclusion, we have demonstrated that LAC2, a laccase produced by the basidiomycete C30 following ... Characterization of a low redox potential laccase from the basidiomycete C30 Agnieszka Klonowska1, Christian Gaudin2,*, Andre´Fournel3, Marcel Asso3, Jean Le Petit2,Michel...
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Báo cáo Y học: Characterization of a partially folded intermediate of stem bromelain at low pH ppt
... Characterization of a partially folded intermediate of stembromelain at low pHSoghra Khatun Haq, Sheeba Rasheedi and Rizwan Hasan KhanInterdisciplinary Biotechnology Unit, Aligarh Muslim ... cysteinyl protease from Ananascomosus has been made till date. A rroyo-Reyna et al. haveproposed that bromelain f orms may have t he same foldingpattern shown by other members of the papain ... is the p artially f oldedstate. A similar intermediate state on the N ® MGpathway, termed the premolten globule state, has beenlocalized at pH 5.0 for the apo -a- lactalbumin by Lala &Kaul...
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Tài liệu Báo cáo Y học: Characterization of a cloned subtilisin-like serine proteinase from a psychrotrophic Vibrio species doc
... E., Sakai, H., Wakagi, T. & Matsuzawa, H.(1999) Weakly bound calcium ions involved in the thermostability of aqualysin I, a heat-stable subtilisin-type protease of Thermusaquaticus YT-1. ... 5¢-ATGTTAAAGAAAGTATTAAGTTGTTG-TATTGCAGC-3¢ and5¢-AAAGTTTGCTTGGAGCGTCAAGCC-ACTGTAAGCCG-3¢, cloned into the expression vector pBAD-TOPO,transformed into the E.coliTop10 strain and grown on LBagar ... on thermal stability and molecularadaptation of proteins, as well as potential candidates forbiotechnological applications. Several enzymes from psy-chrophilic bacteria have now been characterized...
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Tài liệu Báo cáo Y học: Characterization of a novel silkworm (Bombyx mori ) phenol UDP-glucosyltransferase potx
... Technology and Medicine, London SW7 2AZ, UK;2Laboratory of Molecular Entomology and Baculovirology, Riken, Wako, JapanSugar conjugation is a major pathway for the inactivationand excretion of both ... specific enz yme activity.Keywords: Bombyx mori; detoxication; insect; UDP-glycosyltransferase. The UDP-glycosyltransferases ( UGTs) are a superfamily of enzymes that p lay a central role in the detoxication ... [1,4,5].UDP-glucuronosyltransferases are located in the lumen of the endoplasmic reticulu m and are membrane-b ound. The putative transmembrane domain is l ocated near the C-terminus of the protein and only a small...
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Báo cáo Y học: Characterization of a Saccharomyces cerevisiae NADP(H)-dependent alcohol dehydrogenase (ADHVII), a member of the cinnamyl alcohol dehydrogenase family pptx
... Characterization of a Saccharomyces cerevisiaeNADP(H)-dependentalcohol dehydrogenase (ADHVII), a member of the cinnamyl alcoholdehydrogenase familyCarol Larroy, Xavier Pare´s and Josep ... although the kcatandKmvalues with ADHVII are approximately half the values found for ADHVI. The catalytic efficienciestowards the oxidation of cinnamyl alcohol and severalaliphatic alcohols are ... wash the glass beads and the total volume of homogenate was centrifuged at 29 000 g for 1 h. The supernatant was collected and dialysed against buffer A. The dialysed extract was applied to a...
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Báo cáo y học: "Characterization of Human Erythrocytes as Potential Carrier for Pravastatin: An In Vitro Study"
... Characterization of Human Erythrocytes as Potential Carrier for Pravas-tatin: An In Vitro Study Gamal El-din I. Harisa, Mohamed F. Ibrahim, Fars K. Alanazi Department of Pharmaceutics, College of ... College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia Corresponding author: Fars K. Alanazi, Kayyali Chair for Pharmaceutical Industry. afars@ksu.edu.sa © Ivyspring ... pravas-tatin is open hydroxyl-acid so that its hydrophilicity is markedly higher than that of other statins. The oral bioavailability of this statin is low due to degradation in the stomach...
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Báo cáo Y học: Characterization of selenoprotein P as a selenium supply protein docx
... Natl Acad. Sci. U.S .A. 73, 2023–2027.21. Takahashi, K., Akasaka, M., Yamamoto, Y. , Kobayashi, C.,Mizoguchi, J. & Koyama, J. (1990) Primary structure of humanplasma glutathione peroxidase ... metabolism? Proc.Natl Acad. Sci. USA 93, 15086–15091.15. Saito, Y. , Hayashi, T., Tanaka, A. , Watanabe, Y. , Suzuki, M.,Saito, E. & Takahashi, K. (1999) Selenoprotein P in humanplasma as an ... Biophys. 305, 541–545.23. Takebe, G., Yarimizu, J., Saito, Y. , Hayashi, T., Nakamura,H., Yodoi, J., Nagasawa, S. & Takahashi, K. (2002) A com-parative study on the hydroperoxide and thiol...
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Báo cáo Y học: Characterization of heparin binding by a peptide from amyloid P component using capillary electrophoresis, surface plasmon resonance and isothermal titration calorimetry ppt
... to a current of approximately 85 lAat a capillary temperature thermostatting of 20 °C. The capillary was rinsed after electrophoresis for 1 min witheach of the following: 0.1MNaOH,water,andelectro-phoresis ... 523–536.2. Kalaria, R.N., Galloway, P.G. & Perry, G. (1991) Widespreadserum amyloid P immunoreactivity in cortical amyloid depositsand the neurofibrillary pathology of Alzheimer’s disease and otherdegenrative ... volume has passed the sensor chip and empty bufferflows past the sensor surface again. The observed rateconstant, kobs,wasevaluatedfromeachtraceandusedtocalculate the association rate constant...
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Báo cáo y học: " Implementation of a new emergency medical communication centre organization in Finland an evaluation, with performance indicators"
... Probability that was the same or below 0.01 was accepted as statisticallysignificant.Results A total of 67 610 emergency calls were analyzed, and of these, 54 026 (79.9%) were from the municipality-basedcenters, ... from the munici-pality-based databases. The rest of the data could not begathered since it was impossible to retrieve it from the olddatabases. The estimated number of emergency calls in the area ... There was noorganized collection of data that could allow for a struc-tured evaluation of the organizational changes. It is evi-dent that a well-planned evaluation of changes in the organizations,...
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Báo cáo y học: "Gain of a 500-fold sensitivity on an intravital MR Contrast Agent based on an endohedral Gadolinium-Cluster-Fullerene-Conjugate: A new chance in cancer diagnostics"
... radiation therapy (IMRT) and the use of heavy ions demands absolute reliability of new diagnostics and treatment planning for prostate and brain tumors. By the fact that the rare earth metals ... was slowly added at 0–5°C. The resulting deeply colored solution was maintained at room temperature for 4 h. Then the organic phase was washed with water, followed by 1N-HCl and again water. ... 4-(6-(pyrimidine-2-yl)-1,4-dihydro-1,2,4,5-tetrazine-3-yl)benzoic acid 3 was prepared from 2-pyrimidinecarbonitrile 1 and p-cyano benzoic acid 2 by reaction with 85% hydrazine. After purification by recrystalisation the dihydrotetrazine was then oxi-dized...
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