Báo cáo Y học: Assignment of molecular properties of a superactive coagulation factor VIIa variant to individual amino acid changes potx
... Assignment of molecular properties of a superactive coagulation factor VIIa variant to individual amino acid changes Egon Persson 1 and Ole H. Olsen 2 1 Haemostasis Biology and 2 Medicinal ... incubation with cyanate. RESULTS Enzymatic activity of FVIIa variants Two forms of enzymatic activity are analyzed. The amido- lytic activity is measured to assess...
Ngày tải lên: 08/03/2014, 09:20
... assays make use of differing technologies and can generally be divided into (i) signal amplification assays (liquid phase hybridization, antibody capture approach, branched DNA) and (ii) DNA ... ddNTPs by means of a DNA polymerase [34]. An alternative approach involves differential hybridization to a preexisting panel of membrane-bound probes as illustrated by the commercially...
Ngày tải lên: 03/11/2012, 09:41
... acids 7–22 of parabutoporin (an a- helical part having the four amino acids LAKK identical to mastoparan) and of the first 28 amino acids of the opistoporins were synthesized and were investigated ... sequence was unambiguously determined by Edman degradation up to amino acid 42. At position 43 a very weak signal corresponding to a proline appeared. Each sequencing cyc...
Ngày tải lên: 21/02/2014, 15:20
Báo cáo Y học: Purification and properties of the extracellular lipase, LipA, of Acinetobacter sp. RAG-1 docx
... and sequence analysis of the lipase and lipase chaperone- encoding genes from Acinetobacter calcoaceticus RAG-1, and redefinition of a proteobacterial lipase family and an analogous lipase chaperone family. ... dark blue band. Positive indicator of tricaprylin hydrolysis was recorded by appearance of a zone of clearing. Effect of pH on lipase activity and stability Concentrated...
Ngày tải lên: 23/03/2014, 21:20
Báo cáo Y học: Puri®cation and properties of an alkaline proteinase of Fusarium culmorum doc
... connected the amino acids leucine and aspartic acid. Such bonds are not normally cleaved by trypsin, but are by subtilisin. This indicates that these peptides were probably separated by an autolytic e ... for SAAPFpNA. To de®ne the substrate speci®city of the enzyme, its activity was measured in duplicate with the substrates SAAPFpNA, N-succinyl-Ala-Ala-Pro-Leu pNA (SAAPLpNA), N-gluta-...
Ngày tải lên: 31/03/2014, 21:21
Báo cáo Y học: The cold-active lipase of Pseudomonas fragi Heterologous expression, biochemical characterization and molecular modeling pdf
... cold-active lipase of Pseudomonas fragi Heterologous expression, biochemical characterization and molecular modeling Claudia Alquati, Luca De Gioia, Gianluca Santarossa, Lilia Alberghina, Piercarlo ... lipases is the so-called oxyanion hole, an arrangement of amino acids which plays an important role in the stabilization of the tetrahedral intermediate transiently formed in the hydr...
Ngày tải lên: 24/03/2014, 04:21
Báo cáo y học: "Facing medical care problems of victims of sexual violence in Goma/Eastern Democratic Republic of the Congo"
... oxygen saturation, materials to administer neuro-axial anaesthesia, and materials for air- way management. Availability of drugs (including expiry dates), laboratory tests (including expiry dates), ... using a predefined systematic protocol: number of beds, administrative background, availability of a physician specialized in gynaecological surgery or gynaecology, anaesthetist, ps...
Ngày tải lên: 25/10/2012, 10:06
Báo cáo y học: "Excess circulating angiopoietin-2 is a strong predictor of mortality in critically ill medical patients"
... rate; m = male; MAP = mean arterial pressure; PaO 2 = partial pressure of oxygen in arterial blood; SOFA = Sequential Organ Failure Assessment; VEGF = vascular endothelial growth factor. Available ... the same investigator. Quantification of circulating Ang-1 and Ang-2 Ang-1 and Ang-2 were measured by in-house Immuno Radio- metric Sandwich Assay (IRMA) and ELISA, respectively as previ...
Ngày tải lên: 25/10/2012, 10:31
Báo cáo y học: " Derivation and preliminary validation of an administrative claims-based algorithm for the effectiveness of medications for rheumatoid arthritis"
... global scores independent of RA disease activity. As part of a sensitivity analysis, we restricted the cohort to patients without any of these ICD-9 codes as part of a sensitivity analysis. As ... provide a preliminary mechanism to evaluate the effectiveness of RA medications in administrative claims and pharmacy data. While clinical disease activity measures remain th...
Ngày tải lên: 25/10/2012, 10:45
Báo cáo y học: "Translational Medicine and Reliability of Single-Nucleotide Polymorphism Studies: Can We Believe in SNP Reports or Not"
... Cancer Res. 2004 Apr 15;10(8):2618-25 47. Okuno T, Tamura T, Yamamori M, Chayahara N, Yamada T, Miki I, Okamura N, Kadowaki Y, Shirasaka D, Aoyama N, Nakamura T, Okumura K, Azuma T, Kasuga ... polymorphism and survival of patients with meta- static colorectal cancer. J Natl Cancer Inst. 2002;94:936-42 61. Sreeja L, Syamala V, Hariharan S, Syamala VS, Raveendran PB, Sivanandan CD, Mad...
Ngày tải lên: 25/10/2012, 10:51