Báo cáo Y học: Structural analysis of deacylated lipopolysaccharide of Escherichia coli strains 2513 (R4 core-type) and F653 (R3 core-type) pot
Báo cáo Y học: Structural analysis of deacylated lipopolysaccharide of Escherichia coli strains 2513 (R4 core-type) and F653 (R3 core-type) pot
... structure of E. coli R3 and R4 LPS (Eur. J. Biochem. 269) 5991 Structural analysis of deacylated lipopolysaccharide of Escherichia coli strains 2513 (R4 core-type) and F653 (R3 core-type) Sven ... chemicalstructure of deacylated E. coli 2513 LPS (R4 core-type) is asdepicted in Fig. 5. Structural analysis of E. coli F653 core-oligosaccharides (R3 core)We have subjected purified LPS from E. coli F653 ... of the mixture of oligosaccharides prior to HPAEC, of theisolated main oligosaccharide of deacylated LPS and of acylated purified LPS from E. coli F2513 (R4 core-type). In addition, the deacylated...
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Báo cáo Y học: Structural analysis of Francisella tularensis lipopolysaccharide potx
... had unsubstituted hydroxyl group at C-1, and wasmostly present in an a-pyranose form. Acyl1,acyl2 and acyl3residues had hydroxy or acyloxy groups at C-3 (13C signals of C-3 at 69.0–72.4 ... protons and the H-2 of acylresidues, and from HMBC correlations between C-1 of acylgroups and protons at the acylation site (Fig. 1). NOEbetween protons A2 and acyl1-2, and between B 2and acyl2-2 ... 2-N-(3-O-acyl4-acyl2)-b-GlcN-(1–6)-2-N-acyl1)3-O-acyl3-GlcN whereacyl1,acyl2 and acyl3are 3-hydroxyhexadecanoic or 3-hy-droxyoctadecanoic acids, acyl4is tetradecanoic or (minor)hexadecanoic...
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Báo cáo Y học: Structural analysis of the lipopolysaccharide from nontypeable Haemophilus influenzae strain 1003 potx
... amounts of sialylated Hex3 glycoforms were indicated.bMinor amounts of Hex2 and Hex3 glycoforms lacking acetyl groups orcontaining a second glycyl group were indicated.cVery minor amounts of ... Computer-assisted structural analysis of polysaccharides with an extendedversion of CASPER using1H- and 13C-N.M.R. d ata. Carbohydr.Res. 188, 169–191.Ó FEBS 2002 Structural analysis of LPS from ... removal of acetate and glycine, con-firming glycine to be e ster-linked.Information on the location of the a cetyl and glycylgroups as well as glycose sequence was provided by ESItandem mass...
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Báo cáo y học: "Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells"
... biology and biochemistry have enabled analy-sis of the expression profiles of numerous proteins at once. In this study, we looked at hypoxia-induced apoptosis and, by using the Western array ... is a cascade of events that involves ac-tivation of many genes and synthesis of various pro-teins. The importance of several apoptotic pathways such as mitochondrial pathways and death receptor ... expression of Bcl-2 and by modulat-ing phosphorylation of JNK. Both mitochondrial and stress-activated apoptosis pathways played important roles in the hypoxia induced cell death of trophoblastic...
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Báo cáo y học: " Mutation Analysis of hCDC4 in AML Cells Identifies a New Intronic Polymorphis"
... ABI PRISM Big Dye Terminator system (Applied Biosystems, Germany). Sequences were analyzed using the Chromas software (Technely-sium Pty Ltd, Tewantin, Australia) and GeneDoc software (http://www.psc.edu/biomed/genedoc). ... of hCDC4 can also be observed in Acute Myeloid Leukemia (AML) or high risk Myelodysplastic Syndrome (MDS). Therefore we carried out a mutational analysis of the hCDC4 gene in 35 samples of ... Results Mutational analysis of hCDC4 in AML In this study the common exons 2 to 11 and the three known transcript variants of exon 1 of hCDC4 (Figure 1A) were analyzed by direct DNA sequencing...
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Báo cáo y học: " Transcriptome analysis of murine thymocytes reveals age-associated changes in thymic gene expression"
... levels of many of these genes identified in the array analysis. Moreover, Western blot analysis of thymo-cyte lysates also confirmed that IgM increases with age (Figure 3). Flow cytometry analysis ... extraction and array analysis. For each array sample, the RNA was prepared from the purified thymocytes. The thymocytes were processed by using the RNEasy Mini Kit (Qiagen, Valencia, CA). Quality and ... increased numbers of thymic B cells and auto-antibodies may be associated with aging in thymocytes and that autoimmune interactions may play important roles in thymocyte death and thymic involution....
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Tài liệu Báo cáo Y học: Functional analysis of DM64, an antimyotoxic protein with immunoglobulin-like structure from Didelphis marsupialis serum pdf
... potentiated by enzymatic degradation of phospholipids [7,44].The capacity of DM64 to inhibit the myotoxicity inducedby myotoxins I (Asp49) and II (Lys49) from B. asper venomwas analyzed in vivo and ... kit.Controls of 0% and 100% cytotoxicity consisted of medium and 0.1% (v/v) Triton X-100 lysate, respectively.Complex formationComplex formation between myotoxin I or II and DM64was analyzed by native ... effectively neut-ralized the myotoxic and cytotoxic effects of both myotox-ins. Interestingly, DM64 did not inhibit the PLA2activity of myotoxin I nor its intracerebroventricular lethality and...
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Tài liệu Báo cáo Y học: Structural diversity and transcription of class III peroxidases from Arabidopsis thaliana docx
... ortholog of HRPC2 (91% aminoacid identity). Shinmyo et al. [64] have studied the promoteractivity and wound-induction of HRPC and -E types, and of AtPCa and AtPEa. Most remarkably, C2, and only C2,responded ... Differentialactivity and structure of highly similar peroxidases. Spectroscopic,crystallographic, and enzymatic analyses of lignifying Arabidopsisthaliana peroxidase A2 and horseradish peroxidase ... University of Geneva, Switzer-land.8. Reddy, C.A. (1993) An overview of recent advances on thephysiology and molecular biology of lignin peroxidases of Phan-erochaete chrysosporium. J. Biotechnol....
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Tài liệu Báo cáo Y học: Functional analysis of a small heat shock/a-crystallin protein from Artemia franciscana docx
... chaperoning in vitro [66], and disassembly of active unitsfrom an oligomeric (storage) state of a-crystallin wasproposed, upon structural analysis of aA-crystallin by site-directed spin labelling, ... variants produced byp26-ND60 and p26-alpha were pr esent but recognizedpoorly by antip26 antibody. To determine if recognition of p26-ND60 and p26-alpha encoded polypeptides by antibodyto p26 was ... ordinary hydration and temperature, cell maintenance entails a constant and substantial free energy flow [51,52]. Anoxic cysts m ayacquire s ufficient energy to survive b y utilization of guanosine...
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Tài liệu Báo cáo Y học: Structural and biochemical characterization of neuronal calretinin domain I– II (residues 1– 100) Comparison to homologous calbindin D28k domain I–II (residues 1 –93) pdf
... Molecular Biology, University of Debrecen, Hungary;3Department of Inorganic Chemistry, and 4Research Group for Antibiotics, Department of Chemistry, University of Debrecen, Hungary;5International ... Ko¨ve´r3, Gyula Batta4 and Jacek Kuz´nicki1,51Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology, Warsaw, Poland;2Department of Biochemistry and Molecular ... C.P.(1999) A kinetic and stereochemical investigation of the role of lysine-32 in the phenylpyruvate tautomerase activity catalyzed bymacrophage migration inhibitory factor. Biochemistry 38,16024–16033.44....
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