Báo cáo Y học: Structural analysis of deacylated lipopolysaccharide of Escherichia coli strains 2513 (R4 core-type) and F653 (R3 core-type) pot

... structure of E. coli R3 and R4 LPS (Eur. J. Biochem. 269) 5991 Structural analysis of deacylated lipopolysaccharide of Escherichia coli strains 2513 (R4 core-type) and F653 (R3 core-type) Sven ... chemical structure of deacylated E. coli 2513 LPS (R4 core-type) is as depicted in Fig. 5. Structural analysis of E. coli F653 core-...

Ngày tải lên: 08/03/2014, 09:20

... had unsubstituted hydroxyl group at C-1, and was mostly present in an a-pyranose form. Acyl 1 ,acyl 2 and acyl 3 residues had hydroxy or acyloxy groups at C-3 ( 13 C signals of C-3 at 69.0–72.4 ... protons and the H-2 of acyl residues, and from HMBC correlations between C-1 of acyl groups and protons at the acylation site (Fig. 1). NOE between protons A2 and acyl 1 -2, and...

Ngày tải lên: 08/03/2014, 09:20

... amounts of sialylated Hex3 glycoforms were indicated. b Minor amounts of Hex2 and Hex3 glycoforms lacking acetyl groups or containing a second glycyl group were indicated. c Very minor amounts of ... Computer- assisted structural analysis of polysaccharides with an extended version of CASPER using 1 H- and 13 C-N.M.R. d ata. Carbohydr. Res. 188, 169–191. Ó FEBS 2002 Structu...

Ngày tải lên: 31/03/2014, 21:21

... biology and biochemistry have enabled analy- sis of the expression profiles of numerous proteins at once. In this study, we looked at hypoxia-induced apoptosis and, by using the Western array ... is a cascade of events that involves ac- tivation of many genes and synthesis of various pro- teins. The importance of several apoptotic pathways such as mitochondrial pathways...

Ngày tải lên: 31/10/2012, 15:12

... ABI PRISM Big Dye Terminator system (Applied Biosystems, Germany). Sequences were analyzed using the Chromas software (Technely- sium Pty Ltd, Tewantin, Australia) and GeneDoc software (http://www.psc.edu/biomed/genedoc ). ... of hCDC4 can also be observed in Acute Myeloid Leukemia (AML) or high risk Myelodysplastic Syndrome (MDS). Therefore we carried out a mutational analysis o...

Ngày tải lên: 31/10/2012, 16:49

... levels of many of these genes identified in the array analysis. Moreover, Western blot analysis of thymo- cyte lysates also confirmed that IgM increases with age (Figure 3). Flow cytometry analysis ... extraction and array analysis. For each array sample, the RNA was prepared from the purified thymocytes. The thymocytes were processed by using the RNEasy Mini Kit (Qiagen, V...

Ngày tải lên: 03/11/2012, 11:35

... potentiated by enzymatic degradation of phospholipids [7,44]. The capacity of DM64 to inhibit the myotoxicity induced by myotoxins I (Asp49) and II (Lys49) from B. asper venom was analyzed in vivo and ... kit. Controls of 0% and 100% cytotoxicity consisted of medium and 0.1% (v/v) Triton X-100 lysate, respectively. Complex formation Complex formation between myotoxin I or II...

Ngày tải lên: 21/02/2014, 01:21

... ortholog of HRPC2 (91% amino acid identity). Shinmyo et al. [64] have studied the promoter activity and wound-induction of HRPC and -E types, and of AtPCa and AtPEa. Most remarkably, C2, and only C2, responded ... Differential activity and structure of highly similar peroxidases. Spectroscopic, crystallographic, and enzymatic analyses of lignifying Arabidopsis thaliana...

Ngày tải lên: 21/02/2014, 01:21

... chaperoning in vitro [66], and disassembly of active units from an oligomeric (storage) state of a-crystallin was proposed, upon structural analysis of aA-crystallin by site- directed spin labelling, ... variants produced by p26-ND60 and p26-alpha were pr esent but recognized poorly by antip26 antibody. To determine if recognition of p26-ND60 and p26-alpha encoded polypeptid...

Ngày tải lên: 22/02/2014, 04:20

... Molecular Biology, University of Debrecen, Hungary; 3 Department of Inorganic Chemistry, and 4 Research Group for Antibiotics, Department of Chemistry, University of Debrecen, Hungary; 5 International ... Ko¨ve ´ r 3 , Gyula Batta 4 and Jacek Kuz ´ nicki 1,5 1 Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology, Warsaw, Poland; 2 Depar...

Ngày tải lên: 22/02/2014, 07:20