Báo cáo Y học: Solution structure of the Alzheimer amyloid b-peptide (1–42) in an apolar microenvironment Similarity with a virus fusion domain potx

... purity assessed by a MALDI-TOF analysis using a Hewlett Packard G202 5A LD-TOF system mass spectro- meter and a- cyano-4-hydroxycinnamic acid as matrix. Sample preparation It has been shown that a ... [38]. Accordingly, it is possible that the mechanism of membrane interaction and destabilization is different in the case of Ab, but it is fair to say that the similarity...

Ngày tải lên: 08/03/2014, 09:20

... molecule and because of inaccuracies in the starting structure. Although mathemat- ical convergence may be achieved by iterative calculations, the accuracy of the distances obtained remains uncertain. Therefore, ... 2001 geometry, the set of solutions reflects any uncertainty in the calibration as well as alternative fits to the set of distances. The main advantages...

Ngày tải lên: 22/02/2014, 07:20

... carboxy termini in close proximity. As shown by Fig. 5A, the amino terminal end of the C5 domain would be attached to remaining domains of gp120 in the native structure. The carboxy terminal end of ... and target cell membranes is facilitated by the structures of gp41 and CD4 receptor. Specifically, gp41 contains a transmembrane domain that is C-terminal to the e...

Ngày tải lên: 08/03/2014, 16:20

... chromatogram abundant in hydrophilic proteins such as linkers and a significant amount of a- and b-phycocyan ins, and only traces of the allophycocyanins. On the contrary, HPLC a nalysis of bands ... allophycoc yanin holds the bridging pigments between phycobilisomes and the photosynthetic lamellae [6]. Both phycocyanin and allophycocyanin are composed of two polypeptide...

Ngày tải lên: 08/03/2014, 16:20

... changes were Table 1. Sequence alignment of TTTT and VVVV2KE. 1 2 13 24 35 TTTT D TASDAAAAAAL TAANAKAAAEL TAANAAAAAAA TAR VVVV2KE D VASDAKAAAEL VAANAKAAAEL VAANAKAAAEA VARCONH 2 1260 E. Liepinsh ... calculations, and a short distance restraint was artificially introduced between Ala14 H a and Lys19 H N , at the expense of an increased number of distance-restraint violations in...

Ngày tải lên: 31/03/2014, 21:21

... secondary -structure elements are well defined, as also indicated by the rmsd values given in Table 1. Analysis of the Ramachandran plot shows that the dihedral angles / and w for the secondary structure ... (the methyl-CpG-binding domains of rat MeCP2 and methyl- CpG-binding domain protein 1, respectively), exhibiting a three-stranded antiparallel b-sheet and an a- he...

Ngày tải lên: 21/02/2014, 00:20

... polypeptide chain with three domains. Despite the low sequence similarity, the domain organization of plant and fungal ligases is very sim- ilar: an N-terminal adenylyltransferase ⁄ ligase domain, followed ... elements and the N- and C-termini are labeled. (C) Backbone overlay of catalytic domains of goldfish RICH (in cyan) and human CNPase (in purple) showing overall s...

Ngày tải lên: 07/03/2014, 10:20

... wild-type protein in the standard complementation a ssay [48]. Reversal of the mutation-induced changes by xenon-binding One might reasonably assume that the removal of the bulky sidechain of an isoleucine ... that a xenon atom binds into the hydrophobic cavity of HPr(I1 4A) that i s created by the replacement of t he bulky Ile14 by an alanine (Fig. 6). P otent...

Ngày tải lên: 07/03/2014, 16:20

... negative) than the latter. As the C-terminal region is rich in acidic amino acids, if it does have some interaction with the N-terminal domain, the mechanism of regulating the catalytic activity ... Zhou 2 , Mark Bartlam 1 , Qing Guo 1 , Yiwei Liu 1 , Yixin Sun 1 , Yu Gao 1,2 , Sheng Ye 1 , Guangtao Li 2 , Zihe Rao 1 , Boqin Qiang 2 and Jiangang Yuan 2 1 Laboratory of Struc...

Ngày tải lên: 08/03/2014, 22:20

... analysis of PDI revealed that loss of the two cysteines in the C-terminal a domain increased the K m to 30 lm, and loss of an additional cys- teine in the a domain resulted in an increase of the ... is a twist of over 120° in the relative orientations of the a and b domains. In one struc- ture, the catalytic cysteines face each other; in...

Ngày tải lên: 16/03/2014, 04:20