Báo cáo Y học: Concerted regulation of free arachidonic acid

Báo cáo Y học: Concerted regulation of free arachidonic acid and hormone-induced steroid synthesis by acyl-CoA thioesterases and acyl-CoA synthetases in adrenal cells pdf

... Concerted regulation of free arachidonic acid and hormone-induced steroid synthesis by acyl-CoA thioesterases and acyl-CoA synthetases in adrenal cells Paula Maloberti, ... concerted regulatory role of acyl- CoA synthetases and thioesterases in the regulation of steroidogenesis by using triacsin C and NDGA on ACTH- stimulated steroid...

Ngày tải lên : 08/03/2014, 09:20

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Báo cáo Y học: Differential regulation of the Fe-hydrogenase during anaerobic adaptation in the green alga Chlamydomonas reinhardtii pot

... stress by redirect- ing t he synthetic machinery towards the synthesis of s ome enzymes involved in glycolysis or sugar-phosphate metabolism [54]. HydA belongs to a new class of Fe-hydrogenases HydA ... stringency using a PCR fragment as p robe (Fig. 3). They showed the presence of one hybridizing signal of similar intensity in different digestions, suggesting that HydA is enc...

Ngày tải lên : 08/03/2014, 22:20

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Báo cáo Y học: Transcriptional regulation of erythropoiesis Fine tuning of combinatorial multi-domain elements ppt

... EKLF, LMO2 and C/REB binding protein (CBP), and enhances the specificity and stability of binding of the two-finger DNA binding domains to palindromic GATA recognition sequences [27,39]. CF is indispensable ... beginning at mouse E9.5; it is multilineage, generating well-defined erythrocytes that synthesize adult forms of globin and become enucleated, as well as myeloid, megakaryoc...

Ngày tải lên : 08/03/2014, 22:20

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Báo cáo Y học: Amphipathic property of free thiol group contributes to an increase in the catalytic efﬁciency of carboxypeptidase Y pot

... to maintain hydrophobicity and Gly, Ser, Asp and His mutants to provide hydrophilicity. The catalytic roles of Cys341 in carboxypeptidase Y are examined by comparing the kinetic parameters of the ... lL0.4 M sulfosalicylic acid per mL of the enzyme/substrate solu- tion. The initial rates of hydrolysis of dipeptide substrates were measured by quantitating the amount of...

Ngày tải lên : 08/03/2014, 23:20

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Báo cáo Y học: Differential regulation of telomerase activity by six telomerase subunits potx

... morphology after induction of diﬀerentiation of HL-60 cells by dimethylsulfoxide and TPA. (A) HL-60 cells cultured in RPMI for 3 days (control for B), followed by cytospin analysis and staining (·1000). ... activity, we used down- regulation of telomerase by inducing differentiation of HL-60 cells, and up -regulation of telomerase by stimulating proliferation...

Ngày tải lên : 17/03/2014, 23:20

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Tài liệu Báo cáo Y học: The binding of lamin B receptor to chromatin is regulated by phosphorylation in the RS region ppt

... phenomenon in vivo. Stimulation of the binding of LBR to chromatin by phosphorylation by a kinase(s) in a synthetic phase egg cytosol Chromatin binding of beads bearing GST–NK was incr- eased by pretreatment ... Identiﬁcation of chromatin binding domains in the N-terminal region of LBR and analysis of the binding mode. Empty columns: four kinds o f GST fusion protein...

Ngày tải lên : 22/02/2014, 04:20

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Báo cáo Y học: Balanced expression of single subunits in a multisubunit protein, achieved by cell fusion of individual transfectants docx

... major immunoglobulin of external secretions and is composed of four different polypeptide chains produced by two distinct cells. Polymeric IgA (pIgA) is produced by B -cells after assembly of light-, a- and joining ... performed in two individual steps (Fig. 1) by mixing equal number of cells (3 · 10 7 cells) of each fusion partner. Cells were centrifuged (5–10 mi...

Ngày tải lên : 18/03/2014, 01:20

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Báo cáo Y học: Proteolytic action of duodenase is required to induce DNA synthesis in pulmonary artery ﬁbroblasts A role for phosphoinositide 3-kinase pot

... induction of DNA synthesis. Importantly, treatment of cells with duodenase (30 n M ) for 10 min followed by the addition of soybean t rypsin inhibitor (3 mgÆmL )1 , 1 5 min) induced [ 3 H]thymidine incorporation ... investigated the ability of duodenase to induce DNA synthesis in bovine pulmonary artery ﬁbroblasts, attempting to elucidate which PAR subtype and signalling pa...

Ngày tải lên : 24/03/2014, 03:21

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Báo cáo Y học: Bromoperoxidase activity of vanadate-substituted acid phosphatases from Shigella ﬂexneri and Salmonella enterica ser. typhimurium doc

... m M )1 Æcm )1 ). Enzymatic assay of bromoperoxidase activity Assay of PhoN-Sf brominating activity. The brominating activity of the recombinant phosphatases was measured qualitatively by the bromination of 40 ... containing 200 m M Br – and 2 m M H 2 O 2 on a Cary 50 [23]. The kinetic parameters were determined using the ENZYMEKINETICS program from Trinity Software. Assay of Pho...

Ngày tải lên : 24/03/2014, 03:21

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Báo cáo Y học: Irregular spiking in free calcium concentration in single, human platelets Regulation by modulation of the inositol trisphosphate receptors ppt

... Throm- bin-induced spiking was acutely abolished by inhibiting phospholipase C or elevating intracellular cAMP levels, while spiking with sulfhydryl reagents was only partially blocked by cAMP ... from those used by InsP 3 , in a way insensitive to heparin and cAMP. Puff-like characteristics of [Ca 2+ ] i spiking in single platelets To determine the involvement of different Ca...

Ngày tải lên : 17/03/2014, 17:20

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