Báo cáo Y học: Functional reconstitution of the HIV receptors CCR5 and CD4 in liposomes pot
Báo cáo Y học: Functional reconstitution of the HIV receptors CCR5 and CD4 in liposomes pot
... histidines at the C-terminus. A plasmid containing the wild type version of CCR5 was constructed by introducing the coding sequence of CCR5 between the ApaIandHindIII restric- tion sites of a ... between the EcoRI and XbaIsites, and inserting the gene between the corresponding sites into the polylinker of pcDNA3. CD4- his was made by cutting a CD4 His 6 const...
Ngày tải lên: 08/03/2014, 09:20
Báo cáo Y học: NMR structure of the HIV-1 regulatory protein Vpr in H2O/trifluoroethanol Comparison with the Vpr N-terminal (1–51) and C-terminal (52–96) domains pot
... nucleic acids and NCp7. Thus, during the early stages of HIV- 1 replication, binding of NCp7 to the C-terminal domain (80–96)Vpr [8] may expose the N-terminal (1–39) portion of the protein and allow ... analysis of the entire protein Vpr in presence of 30% TFE The structure of Vpr was determined by a simulated annealing protocol and energy minimization using 1...
Ngày tải lên: 31/03/2014, 23:20
Báo cáo Y học: Functional studies of the Synechocystis phycobilisomes organization by high performance liquid chromatography on line with a mass spectrometer docx
... masses of the proteins in each HPLC peak. The peaks eluting within 25 min represented linker p roteins, while the four main peaks between 28 and 33 min were the phyco- cyanins and allophycocyanins ... reduction 7 of the b-phycocyanin component. In contrast, the other three bands contain essentially a -and b-phycocyan in in stoichiometric amounts 8 . Injection of b...
Ngày tải lên: 08/03/2014, 16:20
Báo cáo Y học: Functional analysis of the rat bile salt export pump gene promoter Regulation by bile acids, drugs and endogenous compounds potx
... immediately upstream of the TATA box in the p-126 minimal promoter construct completely abolished the stimulation of luciferase activity by CDCA in HepG2 cells (Fig. 6). Interestingly CDCA reduced the ... activity of the mutant m-126 Luc even below that of the wild-type p-126 Luc. Analysis of the binding affinity of the putative FXRE site for the orphan nuclea...
Ngày tải lên: 17/03/2014, 23:20
Báo cáo Y học: Solution structure of the HIV gp120 C5 Domain pptx
... absence of Ôpeak splittingÕ in the TOCSY spectra of C5 indicates that the trans isomer is the major isomer. Interestingly, the H N and H a line-widths of the region encompassing the prolines and ... carboxy termini in close proximity. As shown by Fig. 5A, the amino terminal end of the C5 domain would be attached to remaining domains of gp120 in the native...
Ngày tải lên: 08/03/2014, 16:20
Báo cáo Y học: Differential regulation of the Fe-hydrogenase during anaerobic adaptation in the green alga Chlamydomonas reinhardtii pot
... transport pathway i s postulated f rom the exogenous donor (ferred- oxin) directly to the H-cluster. Protein sequencing of the enzyme and recombinant expression of HydA in E. coli To verify that the hydA ... that the induction of the anaerobic proteins is the result of a n increased mRNA level. Maize (Zea mays L.) responds to anaerobic stress by redirect- ing t he sy...
Ngày tải lên: 08/03/2014, 22:20
Báo cáo khoa học: Functional expression of the quinoline 2-oxidoreductase genes (qorMSL) in Pseudomonas putida KT2440 pUF1 and in P. putida 86-1 Dqor pUF1 and analysis of the Qor proteins doc
... molybdopterin cytosine dinucleotide form of the molybdenum pyranopterin cofactor; Mo-MGD, molyb- dopterin guanine dinucleotide form of the molybdenum pyranopterin cofactor; MPT, molybdopterin; ... plots. Determination of the metal contents of the Qor proteins, and detection of the nucleotide moiety of the molybdenum cofactor The contents of molybdenum and iron...
Ngày tải lên: 17/03/2014, 10:20
Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx
... the catalytic site in the N-terminal domain of one subunit and the substrate recognition site in the C-terminal domain of the other face each other, thereby bringing together the substrate-binding ... (Arg405, Lys407, Lys413, Lys414, Lys422, Lys428, and Lys430) oriented in the same direction with the heparin-binding domain at the N-terminal end [14]. The othe...
Ngày tải lên: 21/02/2014, 15:20
Báo cáo Y học: Functional expression of human liver cytosolic b-glucosidase in Pichia pastoris Insights into its role in the metabolism of dietary glucosides ppt
... liver. Furthermore, we investigated the speci®city of the human CBG with r espect to the glycone and aglycone moieties, and in particular characterized the ef®ciency of the enzyme in hydrolysing a ... (1997) The crystal structures of Sinapis alba myro- sinase and a covalent glycosyl±enzyme intermediate provide insights into the substrate recogn ition and active...
Ngày tải lên: 08/03/2014, 16:20
Báo cáo Y học: Functional integration of mitochondrial and hydrogenosomal ADP/ATP carriers in the Escherichia coli membrane reveals different biochemical characteristics for plants, mammals and anaerobic chytrids pdf
... dependency] of individual mitochondrial-type AACs in order to identify differences in the function of the various isoforms and to understand the repeated evolution of AAC isoforms in a variety of organisms. We ... affinities for both, ADP and ATP. The corresponding K m values range between 10 and 22 l M . They are only slightly in uenced by CCCP. On the other han...
Ngày tải lên: 18/03/2014, 01:20