Báo cáo Y học: Functional reconstitution of the HIV receptors CCR5 and CD4 in liposomes pot
Báo cáo Y học: Functional reconstitution of the HIV receptors CCR5 and CD4 in liposomes pot
... histidines at the C-terminus. A plasmid containing the wild type version of CCR5 was constructed by introducing the codingsequence of CCR5 between the ApaIandHindIII restric-tion sites of a ... between the EcoRI and XbaIsites, and inserting the gene between the corresponding sites into the polylinker of pcDNA3. CD4- his was made by cutting a CD4 His6construct (kindly donated by M. Marsh, ... membrane proteins allows the study of their behaviour in a membrane in the absence of otherproteins, and the manipulation of their concentration and environment. Unfortunately, no single reconstitution method...
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Báo cáo Y học: NMR structure of the HIV-1 regulatory protein Vpr in H2O/trifluoroethanol Comparison with the Vpr N-terminal (1–51) and C-terminal (52–96) domains pot
... nucleicacids and NCp7. Thus, during the early stages of HIV- 1replication, binding of NCp7 to the C-terminal domain(80–96)Vpr [8] may expose the N-terminal (1–39) portion of the protein and allow ... analysis of the entire protein Vpr in presence of 30% TFE The structure of Vpr was determined by a simulatedannealing protocol and energy minimization using 1420distances constraints including ... betweenVpr and other viral and nonviral protein targets. The importance of Vpr protein–protein interactions has beenclearly demonstrated by the loss of activity of Vprfollowing certain point mutations...
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Báo cáo Y học: Functional studies of the Synechocystis phycobilisomes organization by high performance liquid chromatography on line with a mass spectrometer docx
... masses of the proteins in each HPLC peak. The peakseluting within 25 min represented linker p roteins, while the four main peaks between 28 and 33 min were the phyco-cyanins and allophycocyanins ... reduction7 of the b-phycocyanin component. In contrast, the other three bands contain essentially a -and b-phycocyan in in stoichiometric amounts8. Injection of band1 into the HPLC-ESI-MS system ... affected by the environment in diverse ways [2]. In general the macrostructure of phyco-bilisomes consists of a core (constituted by allophycocyanin)surrounded by phycocyanin and phycoerythrin (whenpresent)...
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Báo cáo Y học: Functional analysis of the rat bile salt export pump gene promoter Regulation by bile acids, drugs and endogenous compounds potx
... immediatelyupstream of the TATA box in the p-126 minimalpromoter construct completely abolished the stimulation of luciferase activity by CDCA in HepG2 cells (Fig. 6).Interestingly CDCA reduced the ... activity of the mutant m-126 Luc even below that of the wild-typep-126 Luc.Analysis of the binding affinity of the putativeFXRE site for the orphan nuclear receptor FXRTo characterize the FXRE ... the Bseppromoter and could therefore potentially be involved in estrogen-mediated modification of Bsep gene transcription. In analogy to the human choline acetyltransferase and the lipoprotein...
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Báo cáo Y học: Solution structure of the HIV gp120 C5 Domain pptx
... absence of Ôpeak splittingÕ in the TOCSY spectra of C5 indicates that the trans isomer is the major isomer. Interestingly, the HN and Haline-widths of the region encompassing the prolines and ... carboxy termini in close proximity. As shownby Fig. 5A, the amino terminal end of the C5 domainwould be attached to remaining domains of gp120 in the native structure. The carboxy terminal end of ... doi:10.1046/j.1432-1033.2002.03187.xadditions of acetyl and amido groups to the amino and carboxy termini, respectively. The identity of the peptidewas verified by mass spectrometry (mass ¼ 2700.9). The wild-type HIV gp41 ectodomain was...
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Báo cáo Y học: Differential regulation of the Fe-hydrogenase during anaerobic adaptation in the green alga Chlamydomonas reinhardtii pot
... transportpathway i s postulated f rom the exogenous donor (ferred-oxin) directly to the H-cluster.Protein sequencing of the enzyme and recombinantexpression of HydA in E. coliTo verify that the hydA ... that the induction of the anaerobicproteins is the result of a n increased mRNA level. Maize(Zea mays L.) responds to anaerobic stress by redirect-ing t he synthetic machinery towards the synthesis ... protein in the chloroplast stroma is indicated by a high content of hydroxylated and basic amino acids in the t ransit peptidesequence [39]. The deduced amino-acid sequence of the mature HydApolypeptide...
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Báo cáo khoa học: Functional expression of the quinoline 2-oxidoreductase genes (qorMSL) in Pseudomonas putida KT2440 pUF1 and in P. putida 86-1 Dqor pUF1 and analysis of the Qor proteins doc
... molybdopterin cytosine dinucleotideform of the molybdenum pyranopterin cofactor; Mo-MGD, molyb-dopterin guanine dinucleotide form of the molybdenum pyranopterincofactor; MPT, molybdopterin; ... plots.Determination of the metal contents of the Qor proteins, and detection of the nucleotide moiety of the molybdenum cofactor The contents of molybdenum and iron were determined byinductively coupled ... Release of CMP upon acidic hydrolysis of the Qor proteins suggested the presence of the MCD form of the pyranopterin cofactor; the CMP contents of the threeenzymes were similar.Keywords: quinoline...
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Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx
... the catalytic site in the N-terminal domain of one subunit and the substrate recognition site in the C-terminal domain of the other face each other, thereby bringing together the substrate-binding ... (Arg405, Lys407,Lys413, Lys414, Lys422, Lys428, and Lys430) oriented in the same direction with the heparin-binding domain at the N-terminal end [14]. The other is a cluster of tryptophans(Trp390, ... (40% similarity) and are28% identical in the C-terminal domain (38% similarity). The catalytic efficiency and heparin-binding functions of the N-terminal domain have been extensively studied[13,14]....
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Báo cáo Y học: Functional expression of human liver cytosolic b-glucosidase in Pichia pastoris Insights into its role in the metabolism of dietary glucosides ppt
... liver. Furthermore, we investigated the speci®city of the human CBG with r espect to the glycone and aglycone moieties, and in particular characterized the ef®ciency of the enzyme in hydrolysing a ... (1997) The crystal structures of Sinapis alba myro-sinase and a covalent glycosyl±enzyme intermediate provideinsights into the substrate recogn ition and active-site machinery of an S-glycosidase. ... (4NPGlc), incubating for 30 min at 37 °C, and measuring the rel ease of 4NP.Protein assays and protein sequencingTotal protein in crude and semipuri®ed samples wasestimated using the Pierce Protein...
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Báo cáo Y học: Functional integration of mitochondrial and hydrogenosomal ADP/ATP carriers in the Escherichia coli membrane reveals different biochemical characteristics for plants, mammals and anaerobic chytrids pdf
... dependency] of individual mitochondrial-type AACs in order to identify differences in the function of the variousisoforms and to understand the repeated evolution of AACisoforms in a variety of organisms.We ... affinities for both, ADP and ATP. The corresponding Kmvalues range between 10 and 22 lM.They are only slightly in uenced by CCCP. On the otherhand, the kinetic data of the hydrogenosomal AAC fromNeocallimastix ... D600reached a value of about12.It was tempting to analyse whether the heterologouslyexpressedAACsintegratedintheE. coli membrane were functional. As phosphatidylglycerol and cardiolipin in the mitochondrial...
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Từ khóa: báo cáo y họcbáo cáo y học cổ truyềnmẫu báo cáo y học cổ truyềnbao cao y hoc colchicinphan ban luan trong bao cao y hoc co truyenseven wonders of the world with pictures and information in urduchuyên đề điện xoay chiều theo dạngNghiên cứu tổ hợp chất chỉ điểm sinh học vWF, VCAM 1, MCP 1, d dimer trong chẩn đoán và tiên lượng nhồi máu não cấpBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhPhát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếTìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinThơ nôm tứ tuyệt trào phúng hồ xuân hươngSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXKiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)Quản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)BT Tieng anh 6 UNIT 2Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘITÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲ